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Repeated forced swim stress enhances CFA-evoked thermal hyperalgesia and affects the expressions of pCREB and c-Fos in the insular cortex.
Neuroscience. 2014 Feb 14; 259:1-11.N

Abstract

Stress affects brain activity and promotes long-term changes in multiple neural systems. Exposure to stressors causes substantial effects on the perception and response to pain. In several animal models, chronic stress produces lasting hyperalgesia. The insular (IC) and anterior cingulate cortices (ACC) are the regions exhibiting most reliable pain-related activity. And the IC and ACC play an important role in pain modulation via the descending pain modulatory system. In the present study we examined the expression of phospho-cAMP response element-binding protein (pCREB) and c-Fos in the IC and ACC after forced swim stress (FS) and complete Freund's adjuvant (CFA) injection to clarify changes in the cerebral cortices that affect the activity of the descending pain modulatory system in the rats with stress-induced hyperalgesia. FS (day 1, 10min; days 2-3, 20min) induced an increase in the expression of pCREB and c-Fos in the anterior IC (AIC). CFA injection into the hindpaw after the FS shows significantly enhanced thermal hyperalgesia and induced a decrease in the expression of c-Fos in the AIC and the posterior IC (PIC). Quantitative image analysis showed that the numbers of c-Fos-immunoreactive neurons in the left AIC and PIC were significantly lower in the FS+CFA group (L AIC, 95.9±6.8; L PIC, 181.9±23.1) than those in the naive group (L AIC, 151.1±19.3, p<0.05; L PIC, 274.2±37.3, p<0.05). These findings suggest a neuroplastic change in the IC after FS, which may be involved in the enhancement of CFA-induced thermal hyperalgesia through dysfunction of the descending pain modulatory system.

Authors+Show Affiliations

Department of Physiology, Wakayama Medical University, Kimiidera 811-1, Wakayama City 641-8509, Japan. Electronic address: imbe@wakayama-med.ac.jp.Department of Physiology, Wakayama Medical University, Kimiidera 811-1, Wakayama City 641-8509, Japan.Department of Physiology, Wakayama Medical University, Kimiidera 811-1, Wakayama City 641-8509, Japan.Department of Physiology, Wakayama Medical University, Kimiidera 811-1, Wakayama City 641-8509, Japan.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24291670

Citation

Imbe, H, et al. "Repeated Forced Swim Stress Enhances CFA-evoked Thermal Hyperalgesia and Affects the Expressions of pCREB and c-Fos in the Insular Cortex." Neuroscience, vol. 259, 2014, pp. 1-11.
Imbe H, Kimura A, Donishi T, et al. Repeated forced swim stress enhances CFA-evoked thermal hyperalgesia and affects the expressions of pCREB and c-Fos in the insular cortex. Neuroscience. 2014;259:1-11.
Imbe, H., Kimura, A., Donishi, T., & Kaneoke, Y. (2014). Repeated forced swim stress enhances CFA-evoked thermal hyperalgesia and affects the expressions of pCREB and c-Fos in the insular cortex. Neuroscience, 259, 1-11. https://doi.org/10.1016/j.neuroscience.2013.11.045
Imbe H, et al. Repeated Forced Swim Stress Enhances CFA-evoked Thermal Hyperalgesia and Affects the Expressions of pCREB and c-Fos in the Insular Cortex. Neuroscience. 2014 Feb 14;259:1-11. PubMed PMID: 24291670.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Repeated forced swim stress enhances CFA-evoked thermal hyperalgesia and affects the expressions of pCREB and c-Fos in the insular cortex. AU - Imbe,H, AU - Kimura,A, AU - Donishi,T, AU - Kaneoke,Y, Y1 - 2013/11/28/ PY - 2013/09/20/received PY - 2013/11/22/revised PY - 2013/11/23/accepted PY - 2013/12/3/entrez PY - 2013/12/3/pubmed PY - 2014/9/12/medline KW - ACC KW - AIC KW - ANOVA KW - BSA KW - CFA KW - FS KW - IC KW - IEG KW - IR KW - PAG KW - PB KW - PFA KW - PIC KW - PWL KW - RAIC KW - RT KW - RVM KW - SIH KW - TBS KW - TTBS KW - Tris-buffered saline KW - Tris-buffered saline containing 0.1% Tween-20 KW - analysis of variance KW - anterior cingulate cortex KW - anterior insular cortex KW - bovine serum albumin KW - c-Fos KW - complete Freund’s adjuvant KW - forced swim stress KW - hyperalgesia KW - immediate early gene KW - immunoreactive KW - insular cortex KW - pCREB KW - paraformaldehyde KW - paw withdrawal latency KW - periaqueductal gray matter KW - phosphate buffer KW - phospho-cAMP response element-binding protein KW - posterior insular cortex KW - room temperature KW - rostral agranular insular cortex KW - rostral ventromedial medulla KW - stress-induced hyperalgesia SP - 1 EP - 11 JF - Neuroscience JO - Neuroscience VL - 259 N2 - Stress affects brain activity and promotes long-term changes in multiple neural systems. Exposure to stressors causes substantial effects on the perception and response to pain. In several animal models, chronic stress produces lasting hyperalgesia. The insular (IC) and anterior cingulate cortices (ACC) are the regions exhibiting most reliable pain-related activity. And the IC and ACC play an important role in pain modulation via the descending pain modulatory system. In the present study we examined the expression of phospho-cAMP response element-binding protein (pCREB) and c-Fos in the IC and ACC after forced swim stress (FS) and complete Freund's adjuvant (CFA) injection to clarify changes in the cerebral cortices that affect the activity of the descending pain modulatory system in the rats with stress-induced hyperalgesia. FS (day 1, 10min; days 2-3, 20min) induced an increase in the expression of pCREB and c-Fos in the anterior IC (AIC). CFA injection into the hindpaw after the FS shows significantly enhanced thermal hyperalgesia and induced a decrease in the expression of c-Fos in the AIC and the posterior IC (PIC). Quantitative image analysis showed that the numbers of c-Fos-immunoreactive neurons in the left AIC and PIC were significantly lower in the FS+CFA group (L AIC, 95.9±6.8; L PIC, 181.9±23.1) than those in the naive group (L AIC, 151.1±19.3, p<0.05; L PIC, 274.2±37.3, p<0.05). These findings suggest a neuroplastic change in the IC after FS, which may be involved in the enhancement of CFA-induced thermal hyperalgesia through dysfunction of the descending pain modulatory system. SN - 1873-7544 UR - https://www.unboundmedicine.com/medline/citation/24291670/Repeated_forced_swim_stress_enhances_CFA_evoked_thermal_hyperalgesia_and_affects_the_expressions_of_pCREB_and_c_Fos_in_the_insular_cortex_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0306-4522(13)00992-5 DB - PRIME DP - Unbound Medicine ER -