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In vitro stability of free and glucuronidated cannabinoids in urine following controlled smoked cannabis.
Anal Bioanal Chem. 2014 Jan; 406(3):785-92.AB

Abstract

Analyte stability is an important factor in urine test interpretation, yet cannabinoid stability data are limited. A comprehensive study of Δ(9)-tetrahydrocannabinol (THC), 11-hydroxy-THC (11-OH-THC), 11-nor-9-carboxy-THC (THCCOOH), cannabidiol, cannabinol, THC-glucuronide, and THCCOOH-glucuronide stabilities in authentic urine was completed. Urine samples after ad libitum cannabis smoking were pooled to prepare low and high pools for each study participant; baseline concentrations were measured within 24 h at room temperature (RT), 4 °C and -20 °C. Stability at RT, 4 °C and -20 °C was evaluated by Friedman tests for up to 1 year. THCCOOH, THC-glucuronide, and THCCOOH-glucuronide were quantified in baseline pools. RT THCCOOH baseline concentrations were significantly higher than -20 °C, but not 4 °C baseline concentrations. After 1 week at RT, THCCOOH increased, THCCOOH-glucuronide decreased, but THC-glucuronide was unchanged. In RT low pool, total THCCOOH (THCCOOH + THCCOOH-glucuronide) was significantly lower after 1 week. At 4 °C, THCCOOH was stable 2 weeks, THCCOOH-glucuronide 1 month and THC-glucuronide for at least 6 months. THCCOOH was stable frozen for 1 year, but 6 months high pool results were significantly higher than baseline; THC-glucuronide and THCCOOH-glucuronide were stable for 6 months. Total THCCOOH was stable 6 months at 4 °C, and frozen 6 months (low) and 1 year (high). THC, cannabidiol and cannabinol were never detected in urine; although not detected initially, 11-OH-THC was detected in 2 low and 3 high pools after 1 week at RT. Substantial THCCOOH-glucuronide deconjugation was observed at RT and 4 °C. Analysis should be conducted within 3 months if non-hydrolyzed THCCOOH or THCCOOH-glucuronide quantification is required.

Authors+Show Affiliations

Chemistry and Drug Metabolism, Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, 251 Bayview Boulevard, Suite 200 Room 05A-721, Baltimore, MD, 21224, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Intramural

Language

eng

PubMed ID

24292435

Citation

Desrosiers, Nathalie A., et al. "In Vitro Stability of Free and Glucuronidated Cannabinoids in Urine Following Controlled Smoked Cannabis." Analytical and Bioanalytical Chemistry, vol. 406, no. 3, 2014, pp. 785-92.
Desrosiers NA, Lee D, Scheidweiler KB, et al. In vitro stability of free and glucuronidated cannabinoids in urine following controlled smoked cannabis. Anal Bioanal Chem. 2014;406(3):785-92.
Desrosiers, N. A., Lee, D., Scheidweiler, K. B., Concheiro-Guisan, M., Gorelick, D. A., & Huestis, M. A. (2014). In vitro stability of free and glucuronidated cannabinoids in urine following controlled smoked cannabis. Analytical and Bioanalytical Chemistry, 406(3), 785-92. https://doi.org/10.1007/s00216-013-7524-7
Desrosiers NA, et al. In Vitro Stability of Free and Glucuronidated Cannabinoids in Urine Following Controlled Smoked Cannabis. Anal Bioanal Chem. 2014;406(3):785-92. PubMed PMID: 24292435.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro stability of free and glucuronidated cannabinoids in urine following controlled smoked cannabis. AU - Desrosiers,Nathalie A, AU - Lee,Dayong, AU - Scheidweiler,Karl B, AU - Concheiro-Guisan,Marta, AU - Gorelick,David A, AU - Huestis,Marilyn A, Y1 - 2013/12/01/ PY - 2013/09/25/received PY - 2013/11/18/accepted PY - 2013/11/15/revised PY - 2013/12/3/entrez PY - 2013/12/3/pubmed PY - 2014/10/14/medline SP - 785 EP - 92 JF - Analytical and bioanalytical chemistry JO - Anal Bioanal Chem VL - 406 IS - 3 N2 - Analyte stability is an important factor in urine test interpretation, yet cannabinoid stability data are limited. A comprehensive study of Δ(9)-tetrahydrocannabinol (THC), 11-hydroxy-THC (11-OH-THC), 11-nor-9-carboxy-THC (THCCOOH), cannabidiol, cannabinol, THC-glucuronide, and THCCOOH-glucuronide stabilities in authentic urine was completed. Urine samples after ad libitum cannabis smoking were pooled to prepare low and high pools for each study participant; baseline concentrations were measured within 24 h at room temperature (RT), 4 °C and -20 °C. Stability at RT, 4 °C and -20 °C was evaluated by Friedman tests for up to 1 year. THCCOOH, THC-glucuronide, and THCCOOH-glucuronide were quantified in baseline pools. RT THCCOOH baseline concentrations were significantly higher than -20 °C, but not 4 °C baseline concentrations. After 1 week at RT, THCCOOH increased, THCCOOH-glucuronide decreased, but THC-glucuronide was unchanged. In RT low pool, total THCCOOH (THCCOOH + THCCOOH-glucuronide) was significantly lower after 1 week. At 4 °C, THCCOOH was stable 2 weeks, THCCOOH-glucuronide 1 month and THC-glucuronide for at least 6 months. THCCOOH was stable frozen for 1 year, but 6 months high pool results were significantly higher than baseline; THC-glucuronide and THCCOOH-glucuronide were stable for 6 months. Total THCCOOH was stable 6 months at 4 °C, and frozen 6 months (low) and 1 year (high). THC, cannabidiol and cannabinol were never detected in urine; although not detected initially, 11-OH-THC was detected in 2 low and 3 high pools after 1 week at RT. Substantial THCCOOH-glucuronide deconjugation was observed at RT and 4 °C. Analysis should be conducted within 3 months if non-hydrolyzed THCCOOH or THCCOOH-glucuronide quantification is required. SN - 1618-2650 UR - https://www.unboundmedicine.com/medline/citation/24292435/In_vitro_stability_of_free_and_glucuronidated_cannabinoids_in_urine_following_controlled_smoked_cannabis_ L2 - https://dx.doi.org/10.1007/s00216-013-7524-7 DB - PRIME DP - Unbound Medicine ER -