Tags

Type your tag names separated by a space and hit enter

Inhibition of the growth of colorado potato beetle larvae by macrocypins, protease inhibitors from the parasol mushroom.
J Agric Food Chem. 2013 Dec 26; 61(51):12499-509.JA

Abstract

Proteins from higher fungi have attracted interest because of their exceptional characteristics. Macrocypins, cysteine protease inhibitors from the parasol mushroom Macrolepiota procera , were evaluated for their adverse effects and their mode of action on the major potato pest Colorado potato beetle (CPB, Leptinotarsa decemlineata Say). They were shown to reduce larval growth when expressed in potato or when their recombinant analogues were added to the diet. Macrocypins target a specific set of digestive cysteine proteases, intestains. Additionally, protein-protein interaction analysis revealed potential targets among other digestive enzymes and proteins related to development and primary metabolism. No effect of dietary macrocypins on gene expression of known adaptation-related digestive enzymes was observed in CPB guts. Macrocypins are the first fungal protease inhibitors to be reported as having a negative effect on growth and development of CPB larvae and could also be evaluated as control agents for other pests.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Smid I
Department of Biotechnology and Systems Biology, National Institute of Biology , Večna pot 111, 1000 Ljubljana, Slovenia.
Gruden K
No affiliation info available
Buh Gašparič M
No affiliation info available
Koruza K
No affiliation info available
Petek M
No affiliation info available
Pohleven J
No affiliation info available
Brzin J
No affiliation info available
Kos J
No affiliation info available
Zel J
No affiliation info available
Sabotič J
No affiliation info available

MeSH

AgaricalesAnimalsColeopteraFungal ProteinsInsect ProteinsLarvaPeptide HydrolasesPlant DiseasesPlants, Genetically ModifiedProtease InhibitorsSolanum tuberosum

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24295324

Citation

Smid, Ida, et al. "Inhibition of the Growth of Colorado Potato Beetle Larvae By Macrocypins, Protease Inhibitors From the Parasol Mushroom." Journal of Agricultural and Food Chemistry, vol. 61, no. 51, 2013, pp. 12499-509.
Smid I, Gruden K, Buh Gašparič M, et al. Inhibition of the growth of colorado potato beetle larvae by macrocypins, protease inhibitors from the parasol mushroom. J Agric Food Chem. 2013;61(51):12499-509.
Smid, I., Gruden, K., Buh Gašparič, M., Koruza, K., Petek, M., Pohleven, J., Brzin, J., Kos, J., Zel, J., & Sabotič, J. (2013). Inhibition of the growth of colorado potato beetle larvae by macrocypins, protease inhibitors from the parasol mushroom. Journal of Agricultural and Food Chemistry, 61(51), 12499-509. https://doi.org/10.1021/jf403615f
Smid I, et al. Inhibition of the Growth of Colorado Potato Beetle Larvae By Macrocypins, Protease Inhibitors From the Parasol Mushroom. J Agric Food Chem. 2013 Dec 26;61(51):12499-509. PubMed PMID: 24295324.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibition of the growth of colorado potato beetle larvae by macrocypins, protease inhibitors from the parasol mushroom. AU - Smid,Ida, AU - Gruden,Kristina, AU - Buh Gašparič,Meti, AU - Koruza,Katarina, AU - Petek,Marko, AU - Pohleven,Jure, AU - Brzin,Jože, AU - Kos,Janko, AU - Zel,Jana, AU - Sabotič,Jerica, Y1 - 2013/12/12/ PY - 2013/12/4/entrez PY - 2013/12/4/pubmed PY - 2014/7/6/medline SP - 12499 EP - 509 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 61 IS - 51 N2 - Proteins from higher fungi have attracted interest because of their exceptional characteristics. Macrocypins, cysteine protease inhibitors from the parasol mushroom Macrolepiota procera , were evaluated for their adverse effects and their mode of action on the major potato pest Colorado potato beetle (CPB, Leptinotarsa decemlineata Say). They were shown to reduce larval growth when expressed in potato or when their recombinant analogues were added to the diet. Macrocypins target a specific set of digestive cysteine proteases, intestains. Additionally, protein-protein interaction analysis revealed potential targets among other digestive enzymes and proteins related to development and primary metabolism. No effect of dietary macrocypins on gene expression of known adaptation-related digestive enzymes was observed in CPB guts. Macrocypins are the first fungal protease inhibitors to be reported as having a negative effect on growth and development of CPB larvae and could also be evaluated as control agents for other pests. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/24295324/Inhibition_of_the_growth_of_colorado_potato_beetle_larvae_by_macrocypins_protease_inhibitors_from_the_parasol_mushroom_ DB - PRIME DP - Unbound Medicine ER -