Tags

Type your tag names separated by a space and hit enter

Simultaneous determination of phenolic compounds in Cynthiana grape (Vitis aestivalis) by high performance liquid chromatography-electrospray ionisation-mass spectrometry.
Food Chem. 2014 Apr 15; 149:15-24.FC

Abstract

Phenolic acids, flavanols, flavonols and stilbenes (PAFFS) were isolated from whole grapes, juice, or pomace and purified using enzymatic hydrolysis. Only anthocyanin mono-glucosides and a few of the oligomers from Cynthiana grape (Vitis aestivalis) were analysed. Flavonoid-anthocyanin mono-glucosides (FA) were isolated using methanol/0.1% hydrochloric acid extraction. In addition, crude extractions of phenolic compounds from Cynthiana grape using 50% methanol, 70% methanol, 50% acetone, 0.01% pectinase, or petroleum ether were also evaluated. Reverse phase high performance liquid chromatography (RP-HPLC) with photodiode array (PDA) detector was used to identify phenolic compounds. A method was developed for simultaneous separation, identification and quantification of both PAFFS and FA. Quantification was performed by the internal standard method using a five points regression graph of the UV-visible absorption data collected at the wavelength of maximum absorbance for each analyte. From whole grape samples nine phenolic compounds were tentatively identified and quantified. The individual phenolic compounds content varied from 3 to 875 mg kg⁻¹ dry weight. For juice, twelve phenolic compounds were identified and quantified. The content varied from 0.07 to 910 mg kg⁻¹ dry weight. For pomace, a total of fifteen phenolic compounds were tentatively identified and quantified. The content varied from 2 mg kg⁻¹ to 198 mg kg⁻¹ dry matter. Results from HPLC analysis of the samples showed that gallic acid and (+)-catechin hydrate were the major phenolic compounds in both whole grapes and pomace. Cyanidin and petunidin 3-O-glucoside were the major anthocyanin glucosides in the juice.

Authors+Show Affiliations

Oklahoma State University, Department of Animal Science, 106 ANSI Bldg, 126 FAPC, Stillwater, OK 74078, United States; Robert M. Kerr Food & Agricultural Products Center, 126 FAPC, Stillwater., OK 74078, United States. Electronic address: lina.ramirez@okstate.edu.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

24295671

Citation

Ramirez-Lopez, L M., et al. "Simultaneous Determination of Phenolic Compounds in Cynthiana Grape (Vitis Aestivalis) By High Performance Liquid Chromatography-electrospray Ionisation-mass Spectrometry." Food Chemistry, vol. 149, 2014, pp. 15-24.
Ramirez-Lopez LM, McGlynn W, Goad CL, et al. Simultaneous determination of phenolic compounds in Cynthiana grape (Vitis aestivalis) by high performance liquid chromatography-electrospray ionisation-mass spectrometry. Food Chem. 2014;149:15-24.
Ramirez-Lopez, L. M., McGlynn, W., Goad, C. L., & Mireles Dewitt, C. A. (2014). Simultaneous determination of phenolic compounds in Cynthiana grape (Vitis aestivalis) by high performance liquid chromatography-electrospray ionisation-mass spectrometry. Food Chemistry, 149, 15-24. https://doi.org/10.1016/j.foodchem.2013.10.078
Ramirez-Lopez LM, et al. Simultaneous Determination of Phenolic Compounds in Cynthiana Grape (Vitis Aestivalis) By High Performance Liquid Chromatography-electrospray Ionisation-mass Spectrometry. Food Chem. 2014 Apr 15;149:15-24. PubMed PMID: 24295671.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous determination of phenolic compounds in Cynthiana grape (Vitis aestivalis) by high performance liquid chromatography-electrospray ionisation-mass spectrometry. AU - Ramirez-Lopez,L M, AU - McGlynn,W, AU - Goad,C L, AU - Mireles Dewitt,C A, Y1 - 2013/10/25/ PY - 2013/05/30/received PY - 2013/10/16/revised PY - 2013/10/17/accepted PY - 2013/12/4/entrez PY - 2013/12/4/pubmed PY - 2014/7/22/medline KW - Cynthiana grape KW - Extraction KW - HPLC–ESI–MS KW - Phenolic compounds SP - 15 EP - 24 JF - Food chemistry JO - Food Chem VL - 149 N2 - Phenolic acids, flavanols, flavonols and stilbenes (PAFFS) were isolated from whole grapes, juice, or pomace and purified using enzymatic hydrolysis. Only anthocyanin mono-glucosides and a few of the oligomers from Cynthiana grape (Vitis aestivalis) were analysed. Flavonoid-anthocyanin mono-glucosides (FA) were isolated using methanol/0.1% hydrochloric acid extraction. In addition, crude extractions of phenolic compounds from Cynthiana grape using 50% methanol, 70% methanol, 50% acetone, 0.01% pectinase, or petroleum ether were also evaluated. Reverse phase high performance liquid chromatography (RP-HPLC) with photodiode array (PDA) detector was used to identify phenolic compounds. A method was developed for simultaneous separation, identification and quantification of both PAFFS and FA. Quantification was performed by the internal standard method using a five points regression graph of the UV-visible absorption data collected at the wavelength of maximum absorbance for each analyte. From whole grape samples nine phenolic compounds were tentatively identified and quantified. The individual phenolic compounds content varied from 3 to 875 mg kg⁻¹ dry weight. For juice, twelve phenolic compounds were identified and quantified. The content varied from 0.07 to 910 mg kg⁻¹ dry weight. For pomace, a total of fifteen phenolic compounds were tentatively identified and quantified. The content varied from 2 mg kg⁻¹ to 198 mg kg⁻¹ dry matter. Results from HPLC analysis of the samples showed that gallic acid and (+)-catechin hydrate were the major phenolic compounds in both whole grapes and pomace. Cyanidin and petunidin 3-O-glucoside were the major anthocyanin glucosides in the juice. SN - 1873-7072 UR - https://www.unboundmedicine.com/medline/citation/24295671/Simultaneous_determination_of_phenolic_compounds_in_Cynthiana_grape__Vitis_aestivalis__by_high_performance_liquid_chromatography_electrospray_ionisation_mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0308-8146(13)01523-9 DB - PRIME DP - Unbound Medicine ER -