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A HPLC-MS/MS method for the simultaneous quantitation of six alkaloids of Rhizoma Corydalis Decumbentis in rat plasma and its application to a pharmacokinetic study.

Abstract

A specific and reliable HPLC-MS/MS method was developed and validated for the simultaneous determination of six alkaloids in rat plasma, jatrorrhizine, berberine, tetrahydropalmatine, protopine, bicuculline and palmatine. The analytes were separated on a C18 column (50mm×2.1mm, 1.8μm) and a triple-quadrupole mass spectrometry equipped with an electrospray ionization (ESI) source was used for detection. The plasma sample was prepared by the simple protein precipitation and the recovery for the six analytes was over 80%. The calibration curves were linear over a concentration range of 0.38-1900.0ng/mL for jatrorrhizine, 0.57-2850.0ng/mL for berberine, 0.32-1600.0ng/mL for tetrahydropalmatine, 0.21-1050.0ng/mL for protopine, 0.34-1700.0ng/mL for bicuculline and 0.22-1100ng/mL for palmatine. The intra-day and inter-day precision was less than 15% and the relative error (RE) was all within ±15%. The validated method was successfully applied to a pharmacokinetics study in rats after oral administration of the extracts of Rhizoma Corydalis Decumbentis (a famous Chinese herb).

Authors+Show Affiliations

College of Science, Nanchang University, No. 999, Xuefu Road, Nanchang 330031, China. Electronic address: liaochuanrong@hotmail.com.College of Pharmacy, Jilin Medical College, No. 5 Jilin Street, Jilin 132013, China.Department of Pharmacology, School of Basic Medical Sciences, Shenyang Medical College, No. 146 Huanghebei Street, Huanggu District, Shenyang 110034, China.Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, No. 24 Heping Road, Xiangfang District, Harbin 150040, China.Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, No. 24 Heping Road, Xiangfang District, Harbin 150040, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24295909

Citation

Liao, Chuanrong, et al. "A HPLC-MS/MS Method for the Simultaneous Quantitation of Six Alkaloids of Rhizoma Corydalis Decumbentis in Rat Plasma and Its Application to a Pharmacokinetic Study." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 944, 2014, pp. 101-6.
Liao C, Chang S, Yin S, et al. A HPLC-MS/MS method for the simultaneous quantitation of six alkaloids of Rhizoma Corydalis Decumbentis in rat plasma and its application to a pharmacokinetic study. J Chromatogr B Analyt Technol Biomed Life Sci. 2014;944:101-6.
Liao, C., Chang, S., Yin, S., Wang, Z., & Meng, Y. (2014). A HPLC-MS/MS method for the simultaneous quantitation of six alkaloids of Rhizoma Corydalis Decumbentis in rat plasma and its application to a pharmacokinetic study. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 944, 101-6. https://doi.org/10.1016/j.jchromb.2013.11.010
Liao C, et al. A HPLC-MS/MS Method for the Simultaneous Quantitation of Six Alkaloids of Rhizoma Corydalis Decumbentis in Rat Plasma and Its Application to a Pharmacokinetic Study. J Chromatogr B Analyt Technol Biomed Life Sci. 2014 Jan 1;944:101-6. PubMed PMID: 24295909.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A HPLC-MS/MS method for the simultaneous quantitation of six alkaloids of Rhizoma Corydalis Decumbentis in rat plasma and its application to a pharmacokinetic study. AU - Liao,Chuanrong, AU - Chang,Sheng, AU - Yin,Shiliang, AU - Wang,Zhibin, AU - Meng,Yonghai, Y1 - 2013/11/15/ PY - 2013/04/29/received PY - 2013/10/23/revised PY - 2013/11/06/accepted PY - 2013/12/4/entrez PY - 2013/12/4/pubmed PY - 2014/7/16/medline KW - Alkaloid KW - HPLC–MS/MS KW - Pharmacokinetics KW - Rat plasma KW - Rhizoma Corydalis Decumbentis SP - 101 EP - 6 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. VL - 944 N2 - A specific and reliable HPLC-MS/MS method was developed and validated for the simultaneous determination of six alkaloids in rat plasma, jatrorrhizine, berberine, tetrahydropalmatine, protopine, bicuculline and palmatine. The analytes were separated on a C18 column (50mm×2.1mm, 1.8μm) and a triple-quadrupole mass spectrometry equipped with an electrospray ionization (ESI) source was used for detection. The plasma sample was prepared by the simple protein precipitation and the recovery for the six analytes was over 80%. The calibration curves were linear over a concentration range of 0.38-1900.0ng/mL for jatrorrhizine, 0.57-2850.0ng/mL for berberine, 0.32-1600.0ng/mL for tetrahydropalmatine, 0.21-1050.0ng/mL for protopine, 0.34-1700.0ng/mL for bicuculline and 0.22-1100ng/mL for palmatine. The intra-day and inter-day precision was less than 15% and the relative error (RE) was all within ±15%. The validated method was successfully applied to a pharmacokinetics study in rats after oral administration of the extracts of Rhizoma Corydalis Decumbentis (a famous Chinese herb). SN - 1873-376X UR - https://www.unboundmedicine.com/medline/citation/24295909/A_HPLC_MS/MS_method_for_the_simultaneous_quantitation_of_six_alkaloids_of_Rhizoma_Corydalis_Decumbentis_in_rat_plasma_and_its_application_to_a_pharmacokinetic_study_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(13)00623-5 DB - PRIME DP - Unbound Medicine ER -