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Predominance of IncL/M and IncF plasmid types among CTX-M-ESBL-producing Escherichia coli and Klebsiella pneumoniae in Bulgarian hospitals.
APMIS. 2014 Jul; 122(7):608-15.A

Abstract

Our objective was to investigate the plasmid replicon-types involved in spread of ESBLs among Bulgarian Klebsiella pneumoniae and Escherichia coli. Sixty-three isolates, with transferable beta-lactam resistance determinants, collected between 2007 and 2009 in six medical institutions, were analysed with respect to their antimicrobial susceptibility, ESBL-, RAPD-, and plasmid replicon-type. Phylogenetic typing and screening for the O25b-ST131 lineage were carried out for E. coli. The predominant ESBLs were CTX-M-15 (81%) among E. coli and CTX-M-3 (58%) among K. pneumoniae. Other sporadically found ESBLs were SHV-12 and TEM-139, and for the first time in Bulgaria, CTX-M-1 and CTX-M-14. Replicon typing revealed that plasmids carrying blaCTX-M-3 exclusively belonged to IncL/M-type, while blaCTX-M-15 was predominantly (94%) associated with IncF-type plasmids. Among E. coli, 59% of the isolates were clonally related. Isolates of that cluster produced CTX-M-15, belonged to the O25b-ST131 lineage, predominantly harboured plasmids with the FIA replicon, and were found in five centres. Among CTX-M-3-producing K. pneumoniae, two prevailing RAPD-types were found, one remained restricted to one centre and the second was found in three centres. The incompatibility groups IncN and IncA/C linked with blaSHV-12 respectively blaTEM-139 were found only once. To the best of our knowledge, this is the first detailed investigation of plasmids carrying ESBL genes among Bulgarian isolates demonstrating wide distribution of conjugative IncF plasmids among CTX-M-15-producing E. coli and IncL/M plasmids among CTX-M-3 positive K. pneumoniae isolates.

Authors+Show Affiliations

Department of Medical Microbiology, Medical University, Sofia, Bulgaria.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24303846

Citation

Markovska, Rumyana, et al. "Predominance of IncL/M and IncF Plasmid Types Among CTX-M-ESBL-producing Escherichia Coli and Klebsiella Pneumoniae in Bulgarian Hospitals." APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica, vol. 122, no. 7, 2014, pp. 608-15.
Markovska R, Schneider I, Ivanova D, et al. Predominance of IncL/M and IncF plasmid types among CTX-M-ESBL-producing Escherichia coli and Klebsiella pneumoniae in Bulgarian hospitals. APMIS. 2014;122(7):608-15.
Markovska, R., Schneider, I., Ivanova, D., Mitov, I., & Bauernfeind, A. (2014). Predominance of IncL/M and IncF plasmid types among CTX-M-ESBL-producing Escherichia coli and Klebsiella pneumoniae in Bulgarian hospitals. APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica, 122(7), 608-15. https://doi.org/10.1111/apm.12204
Markovska R, et al. Predominance of IncL/M and IncF Plasmid Types Among CTX-M-ESBL-producing Escherichia Coli and Klebsiella Pneumoniae in Bulgarian Hospitals. APMIS. 2014;122(7):608-15. PubMed PMID: 24303846.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Predominance of IncL/M and IncF plasmid types among CTX-M-ESBL-producing Escherichia coli and Klebsiella pneumoniae in Bulgarian hospitals. AU - Markovska,Rumyana, AU - Schneider,Ines, AU - Ivanova,Dobrinka, AU - Mitov,Ivan, AU - Bauernfeind,Adolf, Y1 - 2013/12/05/ PY - 2013/05/15/received PY - 2013/08/30/accepted PY - 2013/12/6/entrez PY - 2013/12/7/pubmed PY - 2014/8/22/medline KW - CTX-M KW - Enterobacteriaceae KW - IncF KW - IncL/M KW - replicon typing SP - 608 EP - 15 JF - APMIS : acta pathologica, microbiologica, et immunologica Scandinavica JO - APMIS VL - 122 IS - 7 N2 - Our objective was to investigate the plasmid replicon-types involved in spread of ESBLs among Bulgarian Klebsiella pneumoniae and Escherichia coli. Sixty-three isolates, with transferable beta-lactam resistance determinants, collected between 2007 and 2009 in six medical institutions, were analysed with respect to their antimicrobial susceptibility, ESBL-, RAPD-, and plasmid replicon-type. Phylogenetic typing and screening for the O25b-ST131 lineage were carried out for E. coli. The predominant ESBLs were CTX-M-15 (81%) among E. coli and CTX-M-3 (58%) among K. pneumoniae. Other sporadically found ESBLs were SHV-12 and TEM-139, and for the first time in Bulgaria, CTX-M-1 and CTX-M-14. Replicon typing revealed that plasmids carrying blaCTX-M-3 exclusively belonged to IncL/M-type, while blaCTX-M-15 was predominantly (94%) associated with IncF-type plasmids. Among E. coli, 59% of the isolates were clonally related. Isolates of that cluster produced CTX-M-15, belonged to the O25b-ST131 lineage, predominantly harboured plasmids with the FIA replicon, and were found in five centres. Among CTX-M-3-producing K. pneumoniae, two prevailing RAPD-types were found, one remained restricted to one centre and the second was found in three centres. The incompatibility groups IncN and IncA/C linked with blaSHV-12 respectively blaTEM-139 were found only once. To the best of our knowledge, this is the first detailed investigation of plasmids carrying ESBL genes among Bulgarian isolates demonstrating wide distribution of conjugative IncF plasmids among CTX-M-15-producing E. coli and IncL/M plasmids among CTX-M-3 positive K. pneumoniae isolates. SN - 1600-0463 UR - https://www.unboundmedicine.com/medline/citation/24303846/Predominance_of_IncL/M_and_IncF_plasmid_types_among_CTX_M_ESBL_producing_Escherichia_coli_and_Klebsiella_pneumoniae_in_Bulgarian_hospitals_ L2 - https://doi.org/10.1111/apm.12204 DB - PRIME DP - Unbound Medicine ER -