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Epigallocatechin-3-gallate, a green tea catechin, protects the heart against regional ischemia-reperfusion injuries through activation of RISK survival pathways in rats.
Arch Pharm Res. 2014 Aug; 37(8):1079-85.AP

Abstract

Epigallocatechin-3-gallate (EGCG), the major catechin derived from green tea, has been shown to modulate numerous molecular targets in the setting of inflammation. This study aimed to determine whether EGCG protects against regional myocardial ischemia/reperfusion (I/R) injuries and its underlying mechanisms involving the role of reperfusion injury salvage kinase (RISK) pathways (PI3K-Akt and ERK 1/2) and GSK-3β or apoptotic kinases (p38 and JNK). The rats were subjected to I/R injuries consisting of 30 min ischemia followed by 2 h reperfusion. EGCG (10 mg/kg, intravenously) was administered alone or along with wortmannin (PI3K inhibitor, 0.6 mg/kg, intravenously) 5 min before the onset of reperfusion. Wortmannin was administered 10 min before the reperfusion. Infarct size was measured at the end of the reperfusion. The phosphorylation of Akt, GSK-3β, and MAPK kinases (ERK1/2, P38 and JNK) was determined by Western blotting after 10 min of reperfusion. EGCG reduced the infarct size compared with the control (25.4 ± 9.2 versus 43.2 ± 8.2 %, p < 0.05). Wortmannin alone did not affect the infarct size, but abolished the EGCG-induced infarct size limiting effect, indicating that EGCG may protect the heart by modulating the PI3K-Akt. EGCG significantly enhanced the phosphorylation of Akt and GSK-3β but not ERK1/2, while it reduced that of p38 and JNK. These results suggest that EGCG has a protective effect against regional myocardial I/R injuries through activation of the RISK pathway and attenuation of p38 and JNK. EGCG may have cardioprotective effects in patients undergoing surgeries prone to myocardial I/R injuries.

Authors+Show Affiliations

Department of Anesthesiology and Pain Medicine, Chonnam National University Medical School, 671 Jebong-ro Donggu, Gwangju, 501-757, Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24307060

Citation

Kim, Seok Jai, et al. "Epigallocatechin-3-gallate, a Green Tea Catechin, Protects the Heart Against Regional Ischemia-reperfusion Injuries Through Activation of RISK Survival Pathways in Rats." Archives of Pharmacal Research, vol. 37, no. 8, 2014, pp. 1079-85.
Kim SJ, Li M, Jeong CW, et al. Epigallocatechin-3-gallate, a green tea catechin, protects the heart against regional ischemia-reperfusion injuries through activation of RISK survival pathways in rats. Arch Pharm Res. 2014;37(8):1079-85.
Kim, S. J., Li, M., Jeong, C. W., Bae, H. B., Kwak, S. H., Lee, S. H., Lee, H. J., Heo, B. H., Yook, K. B., & Yoo, K. Y. (2014). Epigallocatechin-3-gallate, a green tea catechin, protects the heart against regional ischemia-reperfusion injuries through activation of RISK survival pathways in rats. Archives of Pharmacal Research, 37(8), 1079-85. https://doi.org/10.1007/s12272-013-0309-x
Kim SJ, et al. Epigallocatechin-3-gallate, a Green Tea Catechin, Protects the Heart Against Regional Ischemia-reperfusion Injuries Through Activation of RISK Survival Pathways in Rats. Arch Pharm Res. 2014;37(8):1079-85. PubMed PMID: 24307060.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Epigallocatechin-3-gallate, a green tea catechin, protects the heart against regional ischemia-reperfusion injuries through activation of RISK survival pathways in rats. AU - Kim,Seok Jai, AU - Li,Mei, AU - Jeong,Cheol Won, AU - Bae,Hong Beom, AU - Kwak,Sang Hyun, AU - Lee,Seong Heon, AU - Lee,Hyun Jung, AU - Heo,Bong Ha, AU - Yook,Keun Bae, AU - Yoo,Kyung Yeon, Y1 - 2013/12/05/ PY - 2013/08/13/received PY - 2013/11/20/accepted PY - 2013/12/6/entrez PY - 2013/12/7/pubmed PY - 2015/4/1/medline SP - 1079 EP - 85 JF - Archives of pharmacal research JO - Arch Pharm Res VL - 37 IS - 8 N2 - Epigallocatechin-3-gallate (EGCG), the major catechin derived from green tea, has been shown to modulate numerous molecular targets in the setting of inflammation. This study aimed to determine whether EGCG protects against regional myocardial ischemia/reperfusion (I/R) injuries and its underlying mechanisms involving the role of reperfusion injury salvage kinase (RISK) pathways (PI3K-Akt and ERK 1/2) and GSK-3β or apoptotic kinases (p38 and JNK). The rats were subjected to I/R injuries consisting of 30 min ischemia followed by 2 h reperfusion. EGCG (10 mg/kg, intravenously) was administered alone or along with wortmannin (PI3K inhibitor, 0.6 mg/kg, intravenously) 5 min before the onset of reperfusion. Wortmannin was administered 10 min before the reperfusion. Infarct size was measured at the end of the reperfusion. The phosphorylation of Akt, GSK-3β, and MAPK kinases (ERK1/2, P38 and JNK) was determined by Western blotting after 10 min of reperfusion. EGCG reduced the infarct size compared with the control (25.4 ± 9.2 versus 43.2 ± 8.2 %, p < 0.05). Wortmannin alone did not affect the infarct size, but abolished the EGCG-induced infarct size limiting effect, indicating that EGCG may protect the heart by modulating the PI3K-Akt. EGCG significantly enhanced the phosphorylation of Akt and GSK-3β but not ERK1/2, while it reduced that of p38 and JNK. These results suggest that EGCG has a protective effect against regional myocardial I/R injuries through activation of the RISK pathway and attenuation of p38 and JNK. EGCG may have cardioprotective effects in patients undergoing surgeries prone to myocardial I/R injuries. SN - 0253-6269 UR - https://www.unboundmedicine.com/medline/citation/24307060/Epigallocatechin_3_gallate_a_green_tea_catechin_protects_the_heart_against_regional_ischemia_reperfusion_injuries_through_activation_of_RISK_survival_pathways_in_rats_ L2 - https://dx.doi.org/10.1007/s12272-013-0309-x DB - PRIME DP - Unbound Medicine ER -