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dsRNA uptake and persistence account for tissue-dependent susceptibility to RNA interference in the migratory locust, Locusta migratoria.
Insect Mol Biol. 2014 Apr; 23(2):175-84.IM

Abstract

RNA interference (RNAi) by introducing double-stranded RNA (dsRNA) is a powerful approach to the analysis of gene function in insects; however, RNAi responses vary dramatically in different insect species and tissues, and the underlying mechanisms remain poorly understood. The migratory locust, a destructive insect pest and a hemimetabolic insect with panoistic ovaries, is considered to be a highly susceptible species to RNAi via dsRNA injection, but its ovary appears to be completely insensitive. In the present study, we showed that dsRNA persisted only briefly in locust haemolymph. The ovariole sheath was permeable to dsRNA, but injected dsRNA was not present in the follicle cells and oocytes. The lack of dsRNA uptake into the follicle cells and oocytes is likely to be the primary factor that contributes to the ineffective RNAi response in locust ovaries. These observations provide insights into tissue-dependent variability of RNAi and help in achieving successful gene silencing in insensitive tissues.

Authors+Show Affiliations

State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24308607

Citation

Ren, D, et al. "DsRNA Uptake and Persistence Account for Tissue-dependent Susceptibility to RNA Interference in the Migratory Locust, Locusta Migratoria." Insect Molecular Biology, vol. 23, no. 2, 2014, pp. 175-84.
Ren D, Cai Z, Song J, et al. DsRNA uptake and persistence account for tissue-dependent susceptibility to RNA interference in the migratory locust, Locusta migratoria. Insect Mol Biol. 2014;23(2):175-84.
Ren, D., Cai, Z., Song, J., Wu, Z., & Zhou, S. (2014). DsRNA uptake and persistence account for tissue-dependent susceptibility to RNA interference in the migratory locust, Locusta migratoria. Insect Molecular Biology, 23(2), 175-84. https://doi.org/10.1111/imb.12074
Ren D, et al. DsRNA Uptake and Persistence Account for Tissue-dependent Susceptibility to RNA Interference in the Migratory Locust, Locusta Migratoria. Insect Mol Biol. 2014;23(2):175-84. PubMed PMID: 24308607.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - dsRNA uptake and persistence account for tissue-dependent susceptibility to RNA interference in the migratory locust, Locusta migratoria. AU - Ren,D, AU - Cai,Z, AU - Song,J, AU - Wu,Z, AU - Zhou,S, Y1 - 2013/12/06/ PY - 2013/12/7/entrez PY - 2013/12/7/pubmed PY - 2014/10/30/medline KW - RNAi KW - dsRNA KW - migratory locust KW - ovary KW - siRNA SP - 175 EP - 84 JF - Insect molecular biology JO - Insect Mol Biol VL - 23 IS - 2 N2 - RNA interference (RNAi) by introducing double-stranded RNA (dsRNA) is a powerful approach to the analysis of gene function in insects; however, RNAi responses vary dramatically in different insect species and tissues, and the underlying mechanisms remain poorly understood. The migratory locust, a destructive insect pest and a hemimetabolic insect with panoistic ovaries, is considered to be a highly susceptible species to RNAi via dsRNA injection, but its ovary appears to be completely insensitive. In the present study, we showed that dsRNA persisted only briefly in locust haemolymph. The ovariole sheath was permeable to dsRNA, but injected dsRNA was not present in the follicle cells and oocytes. The lack of dsRNA uptake into the follicle cells and oocytes is likely to be the primary factor that contributes to the ineffective RNAi response in locust ovaries. These observations provide insights into tissue-dependent variability of RNAi and help in achieving successful gene silencing in insensitive tissues. SN - 1365-2583 UR - https://www.unboundmedicine.com/medline/citation/24308607/dsRNA_uptake_and_persistence_account_for_tissue_dependent_susceptibility_to_RNA_interference_in_the_migratory_locust_Locusta_migratoria_ DB - PRIME DP - Unbound Medicine ER -