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Dietary fish oil reduces systemic inflammation and ameliorates sepsis-induced liver injury by up-regulating the peroxisome proliferator-activated receptor gamma-mediated pathway in septic mice.
J Nutr Biochem. 2014 Jan; 25(1):19-25.JN

Abstract

This study investigated the effect of dietary fish oil on systemic inflammation and hepatic injury in mice with polymicrobial sepsis. Male ICR mice were assigned to a control group (C, n=30) and a fish oil group (FO, n=30). Mice in the C group were fed a semi-purified diet with 10% soybean oil, and those in the FO group were fed a fish oil diet (2.5% fish oil+7.5% soybean oil; w/w). Three weeks later, sepsis was induced by cecal ligation and puncture (CLP), and mice were sacrificed at 0, 6 and 24 h after CLP, respectively. Results showed that compared with C group, the FO group had lower plasma levels of tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-10, and nitrite at 6 and 24 h after CLP. Also, peritoneal lavage fluid concentrations of TNF-α and prostaglandin (PG) E2 were significantly lower at 24 h in the FO than in the C group. The FO group had lower myeloperoxidase activities at 6 h after CLP in various organs. Plasma aminotransferase and alanine aminotransferase activities revealed significantly decreased in the FO group. The DNA-binding activity of peroxisome proliferators-activated receptor gamma (PPARγ) and mRNA expression of I kappaB alpha (IκBα) were up-regulated while nuclear factor (NF)-κB p65 DNA-binding activity, inducible nitric oxide synthase protein expression and the concentration of nitrotyrosine were significantly decreased in the FO group in liver after CLP. These results indicate that dietary fish oil administration may attenuate systemic inflammation and up-regulate hepatic PPARγ DNA-binding activity, which may consequently have ameliorated liver injury in these septic mice.

Authors+Show Affiliations

School of Nutrition and Health Sciences, Taipei Medical University, Taipei 110, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24314861

Citation

Li, Cheng-Chung, et al. "Dietary Fish Oil Reduces Systemic Inflammation and Ameliorates Sepsis-induced Liver Injury By Up-regulating the Peroxisome Proliferator-activated Receptor Gamma-mediated Pathway in Septic Mice." The Journal of Nutritional Biochemistry, vol. 25, no. 1, 2014, pp. 19-25.
Li CC, Yang HT, Hou YC, et al. Dietary fish oil reduces systemic inflammation and ameliorates sepsis-induced liver injury by up-regulating the peroxisome proliferator-activated receptor gamma-mediated pathway in septic mice. J Nutr Biochem. 2014;25(1):19-25.
Li, C. C., Yang, H. T., Hou, Y. C., Chiu, Y. S., & Chiu, W. C. (2014). Dietary fish oil reduces systemic inflammation and ameliorates sepsis-induced liver injury by up-regulating the peroxisome proliferator-activated receptor gamma-mediated pathway in septic mice. The Journal of Nutritional Biochemistry, 25(1), 19-25. https://doi.org/10.1016/j.jnutbio.2013.08.010
Li CC, et al. Dietary Fish Oil Reduces Systemic Inflammation and Ameliorates Sepsis-induced Liver Injury By Up-regulating the Peroxisome Proliferator-activated Receptor Gamma-mediated Pathway in Septic Mice. J Nutr Biochem. 2014;25(1):19-25. PubMed PMID: 24314861.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dietary fish oil reduces systemic inflammation and ameliorates sepsis-induced liver injury by up-regulating the peroxisome proliferator-activated receptor gamma-mediated pathway in septic mice. AU - Li,Cheng-Chung, AU - Yang,Hui-Ting, AU - Hou,Yu-Chen, AU - Chiu,Yen-Shuo, AU - Chiu,Wan-Chun, Y1 - 2013/10/05/ PY - 2013/02/04/received PY - 2013/07/15/revised PY - 2013/08/21/accepted PY - 2013/12/10/entrez PY - 2013/12/10/pubmed PY - 2014/7/30/medline KW - Fish oil KW - Inducible nitric oxide synthase KW - NF-κB p65 KW - Peroxisome proliferators-activated receptor gamma KW - Sepsis SP - 19 EP - 25 JF - The Journal of nutritional biochemistry JO - J Nutr Biochem VL - 25 IS - 1 N2 - This study investigated the effect of dietary fish oil on systemic inflammation and hepatic injury in mice with polymicrobial sepsis. Male ICR mice were assigned to a control group (C, n=30) and a fish oil group (FO, n=30). Mice in the C group were fed a semi-purified diet with 10% soybean oil, and those in the FO group were fed a fish oil diet (2.5% fish oil+7.5% soybean oil; w/w). Three weeks later, sepsis was induced by cecal ligation and puncture (CLP), and mice were sacrificed at 0, 6 and 24 h after CLP, respectively. Results showed that compared with C group, the FO group had lower plasma levels of tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-10, and nitrite at 6 and 24 h after CLP. Also, peritoneal lavage fluid concentrations of TNF-α and prostaglandin (PG) E2 were significantly lower at 24 h in the FO than in the C group. The FO group had lower myeloperoxidase activities at 6 h after CLP in various organs. Plasma aminotransferase and alanine aminotransferase activities revealed significantly decreased in the FO group. The DNA-binding activity of peroxisome proliferators-activated receptor gamma (PPARγ) and mRNA expression of I kappaB alpha (IκBα) were up-regulated while nuclear factor (NF)-κB p65 DNA-binding activity, inducible nitric oxide synthase protein expression and the concentration of nitrotyrosine were significantly decreased in the FO group in liver after CLP. These results indicate that dietary fish oil administration may attenuate systemic inflammation and up-regulate hepatic PPARγ DNA-binding activity, which may consequently have ameliorated liver injury in these septic mice. SN - 1873-4847 UR - https://www.unboundmedicine.com/medline/citation/24314861/Dietary_fish_oil_reduces_systemic_inflammation_and_ameliorates_sepsis_induced_liver_injury_by_up_regulating_the_peroxisome_proliferator_activated_receptor_gamma_mediated_pathway_in_septic_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0955-2863(13)00181-2 DB - PRIME DP - Unbound Medicine ER -