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Expression profiles of genes involved in apoptosis and selenium metabolism in articular cartilage of patients with Kashin-Beck osteoarthritis.
Gene. 2014 Feb 10; 535(2):124-30.GENE

Abstract

Kashin-Beck disease (KBD) is a special type of endemic osteoarthritis. It has been suggested that alterations in selenium metabolism and apoptosis play a role in KBD. However, the underlying molecular mechanism remains largely unclear. We performed a microarray analysis using RNA isolated from cartilages of KBD patients and healthy controls, through Significance Analysis of Microarray (SAM) software. Functional gene networks and crucial molecules associated with differentially expressed genes were investigated via Ingenuity Pathway Analysis (IPA) and hub gene analysis. Quantitative real-time PCR was used to check the validation of chip test. We identified 52 up-regulated apoptosis-related genes and 26 down-regulated selenium-related genes between KBD and controls, and these genes associated with the "MYC-mediated apoptosis signaling pathway". We confirmed the results from array studies with quantitative real-time PCR analysis. Our results suggest that abnormal regulation of selenium metabolism and apoptosis through the MYC mediated signaling pathway contributes to the pathogenesis of KBD, but the relationship between apoptosis gene and selenium gene was not found.

Authors+Show Affiliations

Faculty of Public Health, College of Medicine of Xi'an Jiaotong University, Key Laboratory of Environment and Gene Related Diseases of Ministry Education, Key Laboratory of Trace Elements and Endemic Diseases, Ministry of Health, Xi'an 710061, China; Department of Orthopedics Surgery, The First Affiliated Hospital, College of Medicine of Xi'an Jiaotong University, Xi'an 710061, China.Department of Orthopedics Surgery, The Second Affiliated Hospital, College of Medicine, Xi'an Jiaotong University, Xi'an 710004, China.Faculty of Public Health, College of Medicine of Xi'an Jiaotong University, Key Laboratory of Environment and Gene Related Diseases of Ministry Education, Key Laboratory of Trace Elements and Endemic Diseases, Ministry of Health, Xi'an 710061, China.Faculty of Public Health, College of Medicine of Xi'an Jiaotong University, Key Laboratory of Environment and Gene Related Diseases of Ministry Education, Key Laboratory of Trace Elements and Endemic Diseases, Ministry of Health, Xi'an 710061, China.Faculty of Public Health, College of Medicine of Xi'an Jiaotong University, Key Laboratory of Environment and Gene Related Diseases of Ministry Education, Key Laboratory of Trace Elements and Endemic Diseases, Ministry of Health, Xi'an 710061, China.Institute of Biomedicine, University of Eastern Finland, Kuopio, Finland; Biocenter Kuopio, University of Eastern Finland, Kuopio, Finland.Department of Cellular and Structural Biology, University of Texas Health Sciences Center at San Antonio, 7703 Floyd Curl, San Antonio, TX 78229, USA.Faculty of Public Health, College of Medicine of Xi'an Jiaotong University, Key Laboratory of Environment and Gene Related Diseases of Ministry Education, Key Laboratory of Trace Elements and Endemic Diseases, Ministry of Health, Xi'an 710061, China. Electronic address: Guox@mail.xjtu.edu.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24316489

Citation

Wu, Shi-Xun, et al. "Expression Profiles of Genes Involved in Apoptosis and Selenium Metabolism in Articular Cartilage of Patients With Kashin-Beck Osteoarthritis." Gene, vol. 535, no. 2, 2014, pp. 124-30.
Wu SX, Wang WZ, Zhang F, et al. Expression profiles of genes involved in apoptosis and selenium metabolism in articular cartilage of patients with Kashin-Beck osteoarthritis. Gene. 2014;535(2):124-30.
Wu, S. X., Wang, W. Z., Zhang, F., Wu, C. Y., Dennis, B. S., Qu, C. J., Bai, Y. D., & Guo, X. (2014). Expression profiles of genes involved in apoptosis and selenium metabolism in articular cartilage of patients with Kashin-Beck osteoarthritis. Gene, 535(2), 124-30. https://doi.org/10.1016/j.gene.2013.11.050
Wu SX, et al. Expression Profiles of Genes Involved in Apoptosis and Selenium Metabolism in Articular Cartilage of Patients With Kashin-Beck Osteoarthritis. Gene. 2014 Feb 10;535(2):124-30. PubMed PMID: 24316489.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression profiles of genes involved in apoptosis and selenium metabolism in articular cartilage of patients with Kashin-Beck osteoarthritis. AU - Wu,Shi-Xun, AU - Wang,Wei-Zhuo, AU - Zhang,Feng, AU - Wu,Cui-Yan, AU - Dennis,Bannel S, AU - Qu,Cheng-Juan, AU - Bai,Yi-Dong, AU - Guo,Xiong, Y1 - 2013/12/04/ PY - 2013/08/30/received PY - 2013/11/06/revised PY - 2013/11/20/accepted PY - 2013/12/10/entrez PY - 2013/12/10/pubmed PY - 2014/3/13/medline KW - BIND KW - Biomolecular Interaction Network Database KW - Cartilage KW - DIP KW - Database of Interacting Proteins KW - FDR KW - GPX KW - HPRD KW - Hub gene KW - Human Protein Reference Database KW - IPA KW - Ingenuity Pathway Analysis KW - KBD KW - Kashin–Beck disease KW - MINT KW - Molecular Interaction database KW - PDI KW - PPI KW - SAM KW - Signaling pathway KW - Significance Analysis of Microarray KW - false discovery rate KW - glutathione peroxidases KW - protein–DNA interaction KW - protein–protein interaction SP - 124 EP - 30 JF - Gene JO - Gene VL - 535 IS - 2 N2 - Kashin-Beck disease (KBD) is a special type of endemic osteoarthritis. It has been suggested that alterations in selenium metabolism and apoptosis play a role in KBD. However, the underlying molecular mechanism remains largely unclear. We performed a microarray analysis using RNA isolated from cartilages of KBD patients and healthy controls, through Significance Analysis of Microarray (SAM) software. Functional gene networks and crucial molecules associated with differentially expressed genes were investigated via Ingenuity Pathway Analysis (IPA) and hub gene analysis. Quantitative real-time PCR was used to check the validation of chip test. We identified 52 up-regulated apoptosis-related genes and 26 down-regulated selenium-related genes between KBD and controls, and these genes associated with the "MYC-mediated apoptosis signaling pathway". We confirmed the results from array studies with quantitative real-time PCR analysis. Our results suggest that abnormal regulation of selenium metabolism and apoptosis through the MYC mediated signaling pathway contributes to the pathogenesis of KBD, but the relationship between apoptosis gene and selenium gene was not found. SN - 1879-0038 UR - https://www.unboundmedicine.com/medline/citation/24316489/Expression_profiles_of_genes_involved_in_apoptosis_and_selenium_metabolism_in_articular_cartilage_of_patients_with_Kashin_Beck_osteoarthritis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-1119(13)01576-X DB - PRIME DP - Unbound Medicine ER -