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Activation of an encephalitogenic T lymphocyte line with a cellfree supernatant containing basic protein and I region gene products.
J Immunol. 1987 Jan 15; 138(2):452-9.JI

Abstract

Activation of antigen-specific T lymphocyte lines requires presentation of the relevant antigen by syngeneic accessory cells (AC) that express Class II MHC gene products. To determine if the T cell activation signal was AC associated or was shed into the medium, supernatants from rat thymic AC populations pulsed with guinea pig basic protein (GP-BP) or PPD were used to stimulate resting BP- or PPD-reactive T cell lines derived from Lewis or BN rats. Cellfree supernatants were found to stimulate the T cell lines in an antigen-specific, strain-restricted manner, reflecting the pattern of stimulation observed with intact AC used. The activity of the cellfree supernatant could be enhanced five to 20 times in the presence of activated T lymphocytes, the optimal production occurring over a 6-hr period. The cellfree T lymphocyte activation signal produced in the presence of activated T cell products contained both an antigen-specific, MHC-restricted component (85%) and an antigen-independent, mitogenic component (15%). Supernatants containing GP-BP but not bovine BP or PPD induced highly significant proliferation of the Lewis rat-derived BP-1 T lymphocyte line, and transfer of these supernatant-activated cells produced clinical signs of experimental autoimmune encephalomyelitis (EAE) and DTH reactions to GP-BP. The GP-BP component was not inhibited by either of two monoclonal antibodies directed at determinants on either side of the epitope(s) recognized by BP-1 cells. However, stimulation was inhibited by an anti-I-A but not an anti-I-E monoclonal antibody, suggesting the involvement of a Class II MHC gene product on the T cell activation signal. The supernatant activity could be separated by ultracentrifugation at 100,000 X G for 4 hr into a microsomal pellet and an ultrasupernatant, and both fractions had greatly reduced activity on resting T cells until they were reconstituted by vigorous mixing. These results suggest that T effector cells can be activated by AC-derived microsomal fragments bearing Class II antigens that associate noncovalently with processed antigen. This cellfree signal is sufficient to activate encephalitogenic T lymphocytes to transfer clinical EAE and DTH reactions without need for a direct T cell-AC interaction.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

2432125

Citation

Vandenbark, A A., et al. "Activation of an Encephalitogenic T Lymphocyte Line With a Cellfree Supernatant Containing Basic Protein and I Region Gene Products." Journal of Immunology (Baltimore, Md. : 1950), vol. 138, no. 2, 1987, pp. 452-9.
Vandenbark AA, Teal P, Offner H. Activation of an encephalitogenic T lymphocyte line with a cellfree supernatant containing basic protein and I region gene products. J Immunol. 1987;138(2):452-9.
Vandenbark, A. A., Teal, P., & Offner, H. (1987). Activation of an encephalitogenic T lymphocyte line with a cellfree supernatant containing basic protein and I region gene products. Journal of Immunology (Baltimore, Md. : 1950), 138(2), 452-9.
Vandenbark AA, Teal P, Offner H. Activation of an Encephalitogenic T Lymphocyte Line With a Cellfree Supernatant Containing Basic Protein and I Region Gene Products. J Immunol. 1987 Jan 15;138(2):452-9. PubMed PMID: 2432125.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Activation of an encephalitogenic T lymphocyte line with a cellfree supernatant containing basic protein and I region gene products. AU - Vandenbark,A A, AU - Teal,P, AU - Offner,H, PY - 1987/1/15/pubmed PY - 1987/1/15/medline PY - 1987/1/15/entrez SP - 452 EP - 9 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 138 IS - 2 N2 - Activation of antigen-specific T lymphocyte lines requires presentation of the relevant antigen by syngeneic accessory cells (AC) that express Class II MHC gene products. To determine if the T cell activation signal was AC associated or was shed into the medium, supernatants from rat thymic AC populations pulsed with guinea pig basic protein (GP-BP) or PPD were used to stimulate resting BP- or PPD-reactive T cell lines derived from Lewis or BN rats. Cellfree supernatants were found to stimulate the T cell lines in an antigen-specific, strain-restricted manner, reflecting the pattern of stimulation observed with intact AC used. The activity of the cellfree supernatant could be enhanced five to 20 times in the presence of activated T lymphocytes, the optimal production occurring over a 6-hr period. The cellfree T lymphocyte activation signal produced in the presence of activated T cell products contained both an antigen-specific, MHC-restricted component (85%) and an antigen-independent, mitogenic component (15%). Supernatants containing GP-BP but not bovine BP or PPD induced highly significant proliferation of the Lewis rat-derived BP-1 T lymphocyte line, and transfer of these supernatant-activated cells produced clinical signs of experimental autoimmune encephalomyelitis (EAE) and DTH reactions to GP-BP. The GP-BP component was not inhibited by either of two monoclonal antibodies directed at determinants on either side of the epitope(s) recognized by BP-1 cells. However, stimulation was inhibited by an anti-I-A but not an anti-I-E monoclonal antibody, suggesting the involvement of a Class II MHC gene product on the T cell activation signal. The supernatant activity could be separated by ultracentrifugation at 100,000 X G for 4 hr into a microsomal pellet and an ultrasupernatant, and both fractions had greatly reduced activity on resting T cells until they were reconstituted by vigorous mixing. These results suggest that T effector cells can be activated by AC-derived microsomal fragments bearing Class II antigens that associate noncovalently with processed antigen. This cellfree signal is sufficient to activate encephalitogenic T lymphocytes to transfer clinical EAE and DTH reactions without need for a direct T cell-AC interaction. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/2432125/Activation_of_an_encephalitogenic_T_lymphocyte_line_with_a_cellfree_supernatant_containing_basic_protein_and_I_region_gene_products_ L2 - https://www.jimmunol.org/lookup/pmidlookup?view=long&pmid=2432125 DB - PRIME DP - Unbound Medicine ER -