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A label-free electrochemiluminescence aptasensor for thrombin detection based on host-guest recognition between tris(bipyridine)ruthenium(II)-β-cyclodextrin and aptamer.
Biosens Bioelectron. 2014 Apr 15; 54:547-52.BB

Abstract

An ultrasensitive label-free electrochemiluminescence (ECL) aptasensor for the detection of thrombin was developed based on the specific recognition between tris(bipyridine)ruthenium(II)-β-cyclodextrin (tris(bpyRu)-β-CD) and the anti-thrombin aptamer (aptamer). The NH2-aptamer was first immobilized on the activated glassy carbon electrode (GCE) by coupling interaction. By use of the specific recognition between tris(bpyRu)-β-CD and aptamer, tris(bpyRu)-β-CD was then attached on the surface of GCE. Resulting from the outstanding photoactive properties of tris(bpyRu)-β-CD, the fabricated GCE performed strong ECL signal with the coreactant of 2-(dibutylamino)ethanol (DBAE). However, in the presence of thrombin, aptamer-thrombin bioaffinity complexes were formed, which restricted the recognition activities between aptamer and tris(bpyRu)-β-CD. Thus, fewer tris(bpyRu)-β-CD could be attached on the surface of GCE and led to an obvious decrease of ECL signal. Fortunately, the difference of ECL intensity before and after combination with thrombin was logarithmically linear with the concentration of thrombin in a wide range of 10 nM-1 pM. Meantime, a detection limit of 0.1 pM without any other signal labeling or amplifying procedures indicated that the biosensor performed excellent sensitivity, operability and simplicity.

Authors+Show Affiliations

Department of Chemistry, State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai 200241, PR China.Department of Chemistry, State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai 200241, PR China.Department of Chemistry, State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai 200241, PR China.Department of Chemistry, State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai 200241, PR China.Department of Chemistry, State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai 200241, PR China.Department of Chemistry, State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai 200241, PR China.Department of Chemistry, State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai 200241, PR China. Electronic address: pghe@chem.ecnu.edu.cn.

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24321886

Citation

Chen, Qiong, et al. "A Label-free Electrochemiluminescence Aptasensor for Thrombin Detection Based On Host-guest Recognition Between tris(bipyridine)ruthenium(II)-β-cyclodextrin and Aptamer." Biosensors & Bioelectronics, vol. 54, 2014, pp. 547-52.
Chen Q, Chen H, Zhao Y, et al. A label-free electrochemiluminescence aptasensor for thrombin detection based on host-guest recognition between tris(bipyridine)ruthenium(II)-β-cyclodextrin and aptamer. Biosens Bioelectron. 2014;54:547-52.
Chen, Q., Chen, H., Zhao, Y., Zhang, F., Yang, F., Tang, J., & He, P. (2014). A label-free electrochemiluminescence aptasensor for thrombin detection based on host-guest recognition between tris(bipyridine)ruthenium(II)-β-cyclodextrin and aptamer. Biosensors & Bioelectronics, 54, 547-52. https://doi.org/10.1016/j.bios.2013.11.028
Chen Q, et al. A Label-free Electrochemiluminescence Aptasensor for Thrombin Detection Based On Host-guest Recognition Between tris(bipyridine)ruthenium(II)-β-cyclodextrin and Aptamer. Biosens Bioelectron. 2014 Apr 15;54:547-52. PubMed PMID: 24321886.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A label-free electrochemiluminescence aptasensor for thrombin detection based on host-guest recognition between tris(bipyridine)ruthenium(II)-β-cyclodextrin and aptamer. AU - Chen,Qiong, AU - Chen,Hong, AU - Zhao,Yingying, AU - Zhang,Fan, AU - Yang,Fan, AU - Tang,Jie, AU - He,Pingang, Y1 - 2013/11/15/ PY - 2013/08/28/received PY - 2013/10/30/revised PY - 2013/11/07/accepted PY - 2013/12/11/entrez PY - 2013/12/11/pubmed PY - 2014/9/17/medline KW - Aptasensor KW - Electrochemiluminescence KW - Host–guest recognition KW - Tris(bipyridine)ruthenium(II)-β-cyclodextrin SP - 547 EP - 52 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 54 N2 - An ultrasensitive label-free electrochemiluminescence (ECL) aptasensor for the detection of thrombin was developed based on the specific recognition between tris(bipyridine)ruthenium(II)-β-cyclodextrin (tris(bpyRu)-β-CD) and the anti-thrombin aptamer (aptamer). The NH2-aptamer was first immobilized on the activated glassy carbon electrode (GCE) by coupling interaction. By use of the specific recognition between tris(bpyRu)-β-CD and aptamer, tris(bpyRu)-β-CD was then attached on the surface of GCE. Resulting from the outstanding photoactive properties of tris(bpyRu)-β-CD, the fabricated GCE performed strong ECL signal with the coreactant of 2-(dibutylamino)ethanol (DBAE). However, in the presence of thrombin, aptamer-thrombin bioaffinity complexes were formed, which restricted the recognition activities between aptamer and tris(bpyRu)-β-CD. Thus, fewer tris(bpyRu)-β-CD could be attached on the surface of GCE and led to an obvious decrease of ECL signal. Fortunately, the difference of ECL intensity before and after combination with thrombin was logarithmically linear with the concentration of thrombin in a wide range of 10 nM-1 pM. Meantime, a detection limit of 0.1 pM without any other signal labeling or amplifying procedures indicated that the biosensor performed excellent sensitivity, operability and simplicity. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/24321886/A_label_free_electrochemiluminescence_aptasensor_for_thrombin_detection_based_on_host_guest_recognition_between_tris_bipyridine_ruthenium_II__β_cyclodextrin_and_aptamer_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(13)00806-3 DB - PRIME DP - Unbound Medicine ER -