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Immune profiles of CD4+ lymphocyte subsets in breast cancer tumor draining lymph nodes.
Immunol Lett 2014 Mar-Apr; 158(1-2):57-65IL

Abstract

Tumor draining lymph nodes form the first line of defense against tumor dissemination. Lymphocyte subpopulations activated during anti-tumor response determine the outcome of host-tumor interaction. In the present study we explored the percentages of different subtypes of CD4+ lymphocytes, including regulatory cells (TFR, CD25-, and CD25+ Treg cells), helper subsets (Th1, Th2, Th17, and Tfh cells), and the expression level of their cognate cytokines (IFNγ, IL4, and IL17) in tumor draining lymph nodes of patients with breast cancer, and compared the results between node negative (LN-) and node positive (LN+) patients. Forty seven sentinel and non-sentinel auxiliary lymph nodes with or without tumor involvement were collected from untreated breast cancer patients undergoing surgical resection. Mononuclear cells obtained from fresh homogenized lymph nodes were subjected to surface and intracellular staining by flow cytometry. The results revealed the presence of a newly identified subtype of regulatory T cells, TFR, as well as CD25- Treg cells in TDLNs of the breast cancer patients. In addition, evaluation of different helper and regulatory subgroups of CD4+ T lymphocytes showed that upon metastasis of tumor cells to lymph nodes together with the progression of the disease stage, the immune responses changed from an inflammatory to an inhibitory state, as evidenced by a reduction in pro-inflammatory and anti-tumor cytokines, IL17 and IFNγ, as well as an increase in pro-tumorigenic phenotypes, Th2 and Treg cells. This situation may provide a favorable condition for tumor growth and spread.

Authors+Show Affiliations

Cancer Immunology Group, Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran; Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. Electronic address: faghihz@sums.ac.ir.Cancer Immunology Group, Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. Electronic address: erfanin@sums.ac.ir.Cancer Immunology Group, Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. Electronic address: haghshenasmr@sums.ac.ir.Department of Pathology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. Electronic address: safaeia@sums.ac.ir.Department of Surgery, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. Electronic address: taleiab@sums.ac.ir.Cancer Immunology Group, Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran; Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. Electronic address: ghaderia@sums.ac.ir.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24326038

Citation

Faghih, Zahra, et al. "Immune Profiles of CD4+ Lymphocyte Subsets in Breast Cancer Tumor Draining Lymph Nodes." Immunology Letters, vol. 158, no. 1-2, 2014, pp. 57-65.
Faghih Z, Erfani N, Haghshenas MR, et al. Immune profiles of CD4+ lymphocyte subsets in breast cancer tumor draining lymph nodes. Immunol Lett. 2014;158(1-2):57-65.
Faghih, Z., Erfani, N., Haghshenas, M. R., Safaei, A., Talei, A. R., & Ghaderi, A. (2014). Immune profiles of CD4+ lymphocyte subsets in breast cancer tumor draining lymph nodes. Immunology Letters, 158(1-2), pp. 57-65. doi:10.1016/j.imlet.2013.11.021.
Faghih Z, et al. Immune Profiles of CD4+ Lymphocyte Subsets in Breast Cancer Tumor Draining Lymph Nodes. Immunol Lett. 2014;158(1-2):57-65. PubMed PMID: 24326038.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immune profiles of CD4+ lymphocyte subsets in breast cancer tumor draining lymph nodes. AU - Faghih,Zahra, AU - Erfani,Nasrollah, AU - Haghshenas,Mohammad Reza, AU - Safaei,Akbar, AU - Talei,Abdol-Rasoul, AU - Ghaderi,Abbas, Y1 - 2013/12/08/ PY - 2013/09/17/received PY - 2013/11/30/revised PY - 2013/11/30/accepted PY - 2013/12/12/entrez PY - 2013/12/12/pubmed PY - 2014/12/15/medline KW - Breast cancer KW - CD25− Treg KW - T helper subsets KW - TFR SP - 57 EP - 65 JF - Immunology letters JO - Immunol. Lett. VL - 158 IS - 1-2 N2 - Tumor draining lymph nodes form the first line of defense against tumor dissemination. Lymphocyte subpopulations activated during anti-tumor response determine the outcome of host-tumor interaction. In the present study we explored the percentages of different subtypes of CD4+ lymphocytes, including regulatory cells (TFR, CD25-, and CD25+ Treg cells), helper subsets (Th1, Th2, Th17, and Tfh cells), and the expression level of their cognate cytokines (IFNγ, IL4, and IL17) in tumor draining lymph nodes of patients with breast cancer, and compared the results between node negative (LN-) and node positive (LN+) patients. Forty seven sentinel and non-sentinel auxiliary lymph nodes with or without tumor involvement were collected from untreated breast cancer patients undergoing surgical resection. Mononuclear cells obtained from fresh homogenized lymph nodes were subjected to surface and intracellular staining by flow cytometry. The results revealed the presence of a newly identified subtype of regulatory T cells, TFR, as well as CD25- Treg cells in TDLNs of the breast cancer patients. In addition, evaluation of different helper and regulatory subgroups of CD4+ T lymphocytes showed that upon metastasis of tumor cells to lymph nodes together with the progression of the disease stage, the immune responses changed from an inflammatory to an inhibitory state, as evidenced by a reduction in pro-inflammatory and anti-tumor cytokines, IL17 and IFNγ, as well as an increase in pro-tumorigenic phenotypes, Th2 and Treg cells. This situation may provide a favorable condition for tumor growth and spread. SN - 1879-0542 UR - https://www.unboundmedicine.com/medline/citation/24326038/Immune_profiles_of_CD4+_lymphocyte_subsets_in_breast_cancer_tumor_draining_lymph_nodes_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0165-2478(13)00205-8 DB - PRIME DP - Unbound Medicine ER -