García-Ayuso, Diego, et al. "Sectorial Loss of Retinal Ganglion Cells in Inherited Photoreceptor Degeneration Is Due to RGC Death." The British Journal of Ophthalmology, vol. 98, no. 3, 2014, pp. 396-401.
García-Ayuso D, Salinas-Navarro M, Nadal-Nicolás FM, et al. Sectorial loss of retinal ganglion cells in inherited photoreceptor degeneration is due to RGC death. Br J Ophthalmol. 2014;98(3):396-401.
García-Ayuso, D., Salinas-Navarro, M., Nadal-Nicolás, F. M., Ortín-Martínez, A., Agudo-Barriuso, M., Vidal-Sanz, M., & Villegas-Pérez, M. P. (2014). Sectorial loss of retinal ganglion cells in inherited photoreceptor degeneration is due to RGC death. The British Journal of Ophthalmology, 98(3), 396-401. https://doi.org/10.1136/bjophthalmol-2013-303958
García-Ayuso D, et al. Sectorial Loss of Retinal Ganglion Cells in Inherited Photoreceptor Degeneration Is Due to RGC Death. Br J Ophthalmol. 2014;98(3):396-401. PubMed PMID: 24326325.
TY - JOUR
T1 - Sectorial loss of retinal ganglion cells in inherited photoreceptor degeneration is due to RGC death.
AU - García-Ayuso,Diego,
AU - Salinas-Navarro,Manuel,
AU - Nadal-Nicolás,Francisco Manuel,
AU - Ortín-Martínez,Arturo,
AU - Agudo-Barriuso,Marta,
AU - Vidal-Sanz,Manuel,
AU - Villegas-Pérez,María P,
Y1 - 2013/12/10/
PY - 2013/12/12/entrez
PY - 2013/12/12/pubmed
PY - 2014/4/11/medline
KW - Experimental – animal models
KW - Retina
SP - 396
EP - 401
JF - The British journal of ophthalmology
JO - Br J Ophthalmol
VL - 98
IS - 3
N2 - AIMS: To investigate the cause of retinal ganglion cell (RGC) loss in dystrophic aged Royal College of Surgeons (RCS) rats. METHODS: RCS-p+ (dystrophic) female rats of postnatal times (P365, P450 and P540) and age-matched RCS-p1 rdy+ (non-dystrophic) rats were used. In whole-mounted retinas, RGCs were doubly labelled with Fluorogold (FG) retrogradely transported from the superior colliculi and Brn3a immunohistochemistry. RGC axons were labelled with anti-neurofilament antibodies. Automatic image analysis techniques allowed quantification of the total population of RGCs per retina and construction of isodensity maps to investigate RGC topology. RESULTS: Dystrophic retinas showed at all times studied wedge-shaped sectors devoid of FG(+) and Brn3a(+) RGCs. These sectors were also devoid of neurofilament-labelled axons. The total number of FG(+)RGC and Brn3a(+)RGC per retina was significantly smaller in dystrophic rats at P540, revealing RGC death at this age. The total number of FG(+)RGCs was smaller than those of Brn3a(+)RGCs at P540, indicating a disturbance of the retrograde axonal transport at this age. CONCLUSIONS: RGC double labelling documents that sectorial RGC loss in aged dystrophic RCS rats is mainly due to RGC death, although a deficit of the retrograde axonal transport exists also at the more advanced ages.
SN - 1468-2079
UR - https://www.unboundmedicine.com/medline/citation/24326325/Sectorial_loss_of_retinal_ganglion_cells_in_inherited_photoreceptor_degeneration_is_due_to_RGC_death_
DB - PRIME
DP - Unbound Medicine