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Sectorial loss of retinal ganglion cells in inherited photoreceptor degeneration is due to RGC death.
Br J Ophthalmol. 2014 Mar; 98(3):396-401.BJ

Abstract

AIMS

To investigate the cause of retinal ganglion cell (RGC) loss in dystrophic aged Royal College of Surgeons (RCS) rats.

METHODS

RCS-p+ (dystrophic) female rats of postnatal times (P365, P450 and P540) and age-matched RCS-p1 rdy+ (non-dystrophic) rats were used. In whole-mounted retinas, RGCs were doubly labelled with Fluorogold (FG) retrogradely transported from the superior colliculi and Brn3a immunohistochemistry. RGC axons were labelled with anti-neurofilament antibodies. Automatic image analysis techniques allowed quantification of the total population of RGCs per retina and construction of isodensity maps to investigate RGC topology.

RESULTS

Dystrophic retinas showed at all times studied wedge-shaped sectors devoid of FG(+) and Brn3a(+) RGCs. These sectors were also devoid of neurofilament-labelled axons. The total number of FG(+)RGC and Brn3a(+)RGC per retina was significantly smaller in dystrophic rats at P540, revealing RGC death at this age. The total number of FG(+)RGCs was smaller than those of Brn3a(+)RGCs at P540, indicating a disturbance of the retrograde axonal transport at this age.

CONCLUSIONS

RGC double labelling documents that sectorial RGC loss in aged dystrophic RCS rats is mainly due to RGC death, although a deficit of the retrograde axonal transport exists also at the more advanced ages.

Links

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Authors+Show Affiliations

García-Ayuso D
Laboratorio de Oftalmología Experimental, IMIB, Facultad de Medicina, Universidad de Murcia, , Espinardo, Murcia, Spain.
Salinas-Navarro M
No affiliation info available
Nadal-Nicolás FM
No affiliation info available
Ortín-Martínez A
No affiliation info available
Agudo-Barriuso M
No affiliation info available
Vidal-Sanz M
No affiliation info available
Villegas-Pérez MP
No affiliation info available

MeSH

AnimalsAxonal TransportAxonsCell CountFemaleMicroscopy, FluorescencePhotoreceptor Cells, VertebrateRatsRats, Mutant StrainsRetinal Ganglion CellsRetinitis PigmentosaTranscription Factor Brn-3A

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24326325

Citation

García-Ayuso, Diego, et al. "Sectorial Loss of Retinal Ganglion Cells in Inherited Photoreceptor Degeneration Is Due to RGC Death." The British Journal of Ophthalmology, vol. 98, no. 3, 2014, pp. 396-401.
García-Ayuso D, Salinas-Navarro M, Nadal-Nicolás FM, et al. Sectorial loss of retinal ganglion cells in inherited photoreceptor degeneration is due to RGC death. Br J Ophthalmol. 2014;98(3):396-401.
García-Ayuso, D., Salinas-Navarro, M., Nadal-Nicolás, F. M., Ortín-Martínez, A., Agudo-Barriuso, M., Vidal-Sanz, M., & Villegas-Pérez, M. P. (2014). Sectorial loss of retinal ganglion cells in inherited photoreceptor degeneration is due to RGC death. The British Journal of Ophthalmology, 98(3), 396-401. https://doi.org/10.1136/bjophthalmol-2013-303958
García-Ayuso D, et al. Sectorial Loss of Retinal Ganglion Cells in Inherited Photoreceptor Degeneration Is Due to RGC Death. Br J Ophthalmol. 2014;98(3):396-401. PubMed PMID: 24326325.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sectorial loss of retinal ganglion cells in inherited photoreceptor degeneration is due to RGC death. AU - García-Ayuso,Diego, AU - Salinas-Navarro,Manuel, AU - Nadal-Nicolás,Francisco Manuel, AU - Ortín-Martínez,Arturo, AU - Agudo-Barriuso,Marta, AU - Vidal-Sanz,Manuel, AU - Villegas-Pérez,María P, Y1 - 2013/12/10/ PY - 2013/12/12/entrez PY - 2013/12/12/pubmed PY - 2014/4/11/medline KW - Experimental &#8211 animal models KW - Retina SP - 396 EP - 401 JF - The British journal of ophthalmology JO - Br J Ophthalmol VL - 98 IS - 3 N2 - AIMS: To investigate the cause of retinal ganglion cell (RGC) loss in dystrophic aged Royal College of Surgeons (RCS) rats. METHODS: RCS-p+ (dystrophic) female rats of postnatal times (P365, P450 and P540) and age-matched RCS-p1 rdy+ (non-dystrophic) rats were used. In whole-mounted retinas, RGCs were doubly labelled with Fluorogold (FG) retrogradely transported from the superior colliculi and Brn3a immunohistochemistry. RGC axons were labelled with anti-neurofilament antibodies. Automatic image analysis techniques allowed quantification of the total population of RGCs per retina and construction of isodensity maps to investigate RGC topology. RESULTS: Dystrophic retinas showed at all times studied wedge-shaped sectors devoid of FG(+) and Brn3a(+) RGCs. These sectors were also devoid of neurofilament-labelled axons. The total number of FG(+)RGC and Brn3a(+)RGC per retina was significantly smaller in dystrophic rats at P540, revealing RGC death at this age. The total number of FG(+)RGCs was smaller than those of Brn3a(+)RGCs at P540, indicating a disturbance of the retrograde axonal transport at this age. CONCLUSIONS: RGC double labelling documents that sectorial RGC loss in aged dystrophic RCS rats is mainly due to RGC death, although a deficit of the retrograde axonal transport exists also at the more advanced ages. SN - 1468-2079 UR - https://www.unboundmedicine.com/medline/citation/24326325/Sectorial_loss_of_retinal_ganglion_cells_in_inherited_photoreceptor_degeneration_is_due_to_RGC_death_ DB - PRIME DP - Unbound Medicine ER -