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Effect of biobanking conditions on short-term stability of biomarkers in human serum and plasma.
Clin Chem Lab Med 2014; 52(5):629-39CC

Abstract

BACKGROUND

Liquid biobanking is an important tool for laboratory diagnostics in routine settings and clinical studies. However, the current knowledge about adequate storage conditions for different classes of biomarkers is incomplete and, in part, contradictory. Here, we performed a comprehensive study on the effects of different storage conditions on the stability of various biomarkers in human serum and plasma.

METHODS

Serum and citrated plasma were aliquoted and stored at 4 °C, -20 °C, -80 °C, and <-130 °C for 0, 7, 30, and 90 days, respectively (5-10 pools/condition). Additionally, frozen aliquots were temporarily exposed to higher temperatures during storage to simulate removing individual samples. Stability was tested for 32 biomarkers from 10 different parameter classes (electrolytes, enzymes, metabolites, inert proteins, complement factors, ketone bodies, hormones, cytokines, coagulation factors, and sterols).

RESULTS

Biobanking at -80 °C and <-130 °C for up to 90 days did not lead to substantial changes (defined as >3 interassay coefficients of variation and p<0.01) of any biomarker concentration. In contrast, storage at 4 °C and -20 °C induced substantial changes in single biomarker concentrations in most classes. Such substantial changes were increases (<20%) in electrolytes, metabolites, and proteins, and decreases (<96%) in enzymes, ketone bodies, cytokines, and coagulation factors. Biomarker stability was minimally affected by occasional short-term thermal exposure.

CONCLUSIONS

Based on these results, we provide recommendations for storage conditions of up to 90 days for several biomarkers. Generally, storage at ≤-80 °C for at least 90 days including occasional short-term thermal exposure is an excellent storage condition for most biomarkers.

Authors

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Pub Type(s)

Journal Article

Language

eng

PubMed ID

24327528

Citation

Zander, Johannes, et al. "Effect of Biobanking Conditions On Short-term Stability of Biomarkers in Human Serum and Plasma." Clinical Chemistry and Laboratory Medicine, vol. 52, no. 5, 2014, pp. 629-39.
Zander J, Bruegel M, Kleinhempel A, et al. Effect of biobanking conditions on short-term stability of biomarkers in human serum and plasma. Clin Chem Lab Med. 2014;52(5):629-39.
Zander, J., Bruegel, M., Kleinhempel, A., Becker, S., Petros, S., Kortz, L., ... Teupser, D. (2014). Effect of biobanking conditions on short-term stability of biomarkers in human serum and plasma. Clinical Chemistry and Laboratory Medicine, 52(5), pp. 629-39. doi:10.1515/cclm-2013-0705.
Zander J, et al. Effect of Biobanking Conditions On Short-term Stability of Biomarkers in Human Serum and Plasma. Clin Chem Lab Med. 2014;52(5):629-39. PubMed PMID: 24327528.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of biobanking conditions on short-term stability of biomarkers in human serum and plasma. AU - Zander,Johannes, AU - Bruegel,Mathias, AU - Kleinhempel,Alisa, AU - Becker,Susen, AU - Petros,Sirak, AU - Kortz,Linda, AU - Dorow,Juliane, AU - Kratzsch,Jürgen, AU - Baber,Ronny, AU - Ceglarek,Uta, AU - Thiery,Joachim, AU - Teupser,Daniel, PY - 2013/08/28/received PY - 2013/11/06/accepted PY - 2013/12/12/entrez PY - 2013/12/12/pubmed PY - 2014/12/15/medline SP - 629 EP - 39 JF - Clinical chemistry and laboratory medicine JO - Clin. Chem. Lab. Med. VL - 52 IS - 5 N2 - BACKGROUND: Liquid biobanking is an important tool for laboratory diagnostics in routine settings and clinical studies. However, the current knowledge about adequate storage conditions for different classes of biomarkers is incomplete and, in part, contradictory. Here, we performed a comprehensive study on the effects of different storage conditions on the stability of various biomarkers in human serum and plasma. METHODS: Serum and citrated plasma were aliquoted and stored at 4 °C, -20 °C, -80 °C, and <-130 °C for 0, 7, 30, and 90 days, respectively (5-10 pools/condition). Additionally, frozen aliquots were temporarily exposed to higher temperatures during storage to simulate removing individual samples. Stability was tested for 32 biomarkers from 10 different parameter classes (electrolytes, enzymes, metabolites, inert proteins, complement factors, ketone bodies, hormones, cytokines, coagulation factors, and sterols). RESULTS: Biobanking at -80 °C and <-130 °C for up to 90 days did not lead to substantial changes (defined as >3 interassay coefficients of variation and p<0.01) of any biomarker concentration. In contrast, storage at 4 °C and -20 °C induced substantial changes in single biomarker concentrations in most classes. Such substantial changes were increases (<20%) in electrolytes, metabolites, and proteins, and decreases (<96%) in enzymes, ketone bodies, cytokines, and coagulation factors. Biomarker stability was minimally affected by occasional short-term thermal exposure. CONCLUSIONS: Based on these results, we provide recommendations for storage conditions of up to 90 days for several biomarkers. Generally, storage at ≤-80 °C for at least 90 days including occasional short-term thermal exposure is an excellent storage condition for most biomarkers. SN - 1437-4331 UR - https://www.unboundmedicine.com/medline/citation/24327528/Effect_of_biobanking_conditions_on_short_term_stability_of_biomarkers_in_human_serum_and_plasma_ L2 - https://www.degruyter.com/doi/10.1515/cclm-2013-0705 DB - PRIME DP - Unbound Medicine ER -