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Proapoptotic and antimetastatic properties of supercritical CO2 extract of Nigella sativa Linn. against breast cancer cells.
J Med Food. 2013 Dec; 16(12):1121-30.JM

Abstract

Nigella sativa, commonly referred as black cumin, is a popular spice that has been used since the ancient Egyptians. It has traditionally been used for treatment of various human ailments ranging from fever to intestinal disturbances to cancer. This study investigated the apoptotic, antimetastatic, and anticancer activities of supercritical carbon dioxide (SC-CO2) extracts of the seeds of N. sativa Linn. against estrogen-dependent human breast cancer cells (MCF-7). Twelve extracts were prepared from N. sativa seeds using the SC-CO2 extraction method by varying pressure and temperature. Extracts were analyzed using FTIR and UV-Vis spectrometry. Cytotoxicity of the extracts was evaluated on various human cancer and normal cell lines. Of the 12 extracts, 1 extract (A3) that was prepared at 60°C and 2500 psi (~17.24 MPa) showed selective antiproliferative activity against MCF-7 cells with an IC50 of 53.34±2.15 μg/mL. Induction of apoptosis was confirmed by evaluating caspases activities and observing the cells under a scanning electron microscope. In vitro antimetastatic properties of A3 were investigated by colony formation, cell migration, and cell invasion assays. The elevated levels of caspases in A3 treated MCF-7 cells suggest that A3 is proapoptotic. Further nuclear condensation and fragmentation studies confirmed that A3 induces cytotoxicity through the apoptosis pathway. A3 also demonstrated remarkable inhibition in migration and invasion assays of MCF-7 cells at subcytotoxic concentrations. Thus, this study highlights the therapeutic potentials of SC-CO2 extract of N. sativa in targeting breast cancer.

Authors+Show Affiliations

1 EMAN Testing and Research Laboratory, Department of Pharmacology, Universiti Sains Malaysia , Minden, Malaysia .No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24328702

Citation

Baharetha, Hussein M., et al. "Proapoptotic and Antimetastatic Properties of Supercritical CO2 Extract of Nigella Sativa Linn. Against Breast Cancer Cells." Journal of Medicinal Food, vol. 16, no. 12, 2013, pp. 1121-30.
Baharetha HM, Nassar ZD, Aisha AF, et al. Proapoptotic and antimetastatic properties of supercritical CO2 extract of Nigella sativa Linn. against breast cancer cells. J Med Food. 2013;16(12):1121-30.
Baharetha, H. M., Nassar, Z. D., Aisha, A. F., Ahamed, M. B., Al-Suede, F. S., Abd Kadir, M. O., Ismail, Z., & Majid, A. M. (2013). Proapoptotic and antimetastatic properties of supercritical CO2 extract of Nigella sativa Linn. against breast cancer cells. Journal of Medicinal Food, 16(12), 1121-30. https://doi.org/10.1089/jmf.2012.2624
Baharetha HM, et al. Proapoptotic and Antimetastatic Properties of Supercritical CO2 Extract of Nigella Sativa Linn. Against Breast Cancer Cells. J Med Food. 2013;16(12):1121-30. PubMed PMID: 24328702.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Proapoptotic and antimetastatic properties of supercritical CO2 extract of Nigella sativa Linn. against breast cancer cells. AU - Baharetha,Hussein M, AU - Nassar,Zeyad D, AU - Aisha,Abdalrahim F, AU - Ahamed,Mohamed B Khadeer, AU - Al-Suede,Foaud Saleih R, AU - Abd Kadir,Mohd Omar, AU - Ismail,Zhari, AU - Majid,Amin Malik Shah Abdul, PY - 2013/12/17/entrez PY - 2013/12/18/pubmed PY - 2014/9/12/medline SP - 1121 EP - 30 JF - Journal of medicinal food JO - J Med Food VL - 16 IS - 12 N2 - Nigella sativa, commonly referred as black cumin, is a popular spice that has been used since the ancient Egyptians. It has traditionally been used for treatment of various human ailments ranging from fever to intestinal disturbances to cancer. This study investigated the apoptotic, antimetastatic, and anticancer activities of supercritical carbon dioxide (SC-CO2) extracts of the seeds of N. sativa Linn. against estrogen-dependent human breast cancer cells (MCF-7). Twelve extracts were prepared from N. sativa seeds using the SC-CO2 extraction method by varying pressure and temperature. Extracts were analyzed using FTIR and UV-Vis spectrometry. Cytotoxicity of the extracts was evaluated on various human cancer and normal cell lines. Of the 12 extracts, 1 extract (A3) that was prepared at 60°C and 2500 psi (~17.24 MPa) showed selective antiproliferative activity against MCF-7 cells with an IC50 of 53.34±2.15 μg/mL. Induction of apoptosis was confirmed by evaluating caspases activities and observing the cells under a scanning electron microscope. In vitro antimetastatic properties of A3 were investigated by colony formation, cell migration, and cell invasion assays. The elevated levels of caspases in A3 treated MCF-7 cells suggest that A3 is proapoptotic. Further nuclear condensation and fragmentation studies confirmed that A3 induces cytotoxicity through the apoptosis pathway. A3 also demonstrated remarkable inhibition in migration and invasion assays of MCF-7 cells at subcytotoxic concentrations. Thus, this study highlights the therapeutic potentials of SC-CO2 extract of N. sativa in targeting breast cancer. SN - 1557-7600 UR - https://www.unboundmedicine.com/medline/citation/24328702/Proapoptotic_and_antimetastatic_properties_of_supercritical_CO2_extract_of_Nigella_sativa_Linn__against_breast_cancer_cells_ L2 - https://www.liebertpub.com/doi/10.1089/jmf.2012.2624?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -