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Induction of micronuclei and nuclear lesions in Channa punctatus following exposure to carbosulfan, glyphosate and atrazine.
Drug Chem Toxicol. 2014 Oct; 37(4):370-7.DC

Abstract

The genotoxic effects of commonly used agricultural pesticides viz., carbosulfan, glyphosate, and atrazine, were evaluated in Channa punctatus (Pisces, Perciformes) using micronucleus (MN) test and induction of nuclear lesions (NL). The 96 h LC50 value were estimated by probit analysis as 0.27, 32.0 and 42.0 mg L(-1), respectively, for carbosulfan, glyphosate, and atrazine using semi-static bioassays. Based on these values, three sublethal test concentrations of carbosulfan (0.07, 0.13, 0.20 mg L(-1)), glyphosate (8.1, 16.3, 24.4 mg L(-1)) and atrazine (10.6, 21.2, 31.8 mg L(-1)) corresponding to ¼, ½ and ¾ of the LC50 of the pesticides respectively, were selected for exposure for 96 h. Peripheral blood samplings were taken at intervals of 24 h for assessment of MN and NL frequencies. Considerably higher genotoxic damage was induced by carbosulfan as compared to glyphosate and atrazine. There were significant effects (p < 0.01) of concentrations in all the treated groups. The induction of MN and NL was highest at 96 h pesticide exposure at all test concentrations. The nuclear abnormalities recorded in this study, such as blebbed-, lobed-, notched- and bi-nuclei, other than micronuclei, are indicators of genotoxic damage.

Authors+Show Affiliations

Molecular Biology and Biotechnology Division, Department of Zoology and Environmental Biology, University of Nigeria Nsukka , Enugu State , Nigeria .No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24328755

Citation

Nwani, Christopher Didigwu, et al. "Induction of Micronuclei and Nuclear Lesions in Channa Punctatus Following Exposure to Carbosulfan, Glyphosate and Atrazine." Drug and Chemical Toxicology, vol. 37, no. 4, 2014, pp. 370-7.
Nwani CD, Nagpure NS, Kumar R, et al. Induction of micronuclei and nuclear lesions in Channa punctatus following exposure to carbosulfan, glyphosate and atrazine. Drug Chem Toxicol. 2014;37(4):370-7.
Nwani, C. D., Nagpure, N. S., Kumar, R., Kushwaha, B., Kumar, P., & Lakra, W. S. (2014). Induction of micronuclei and nuclear lesions in Channa punctatus following exposure to carbosulfan, glyphosate and atrazine. Drug and Chemical Toxicology, 37(4), 370-7. https://doi.org/10.3109/01480545.2013.866138
Nwani CD, et al. Induction of Micronuclei and Nuclear Lesions in Channa Punctatus Following Exposure to Carbosulfan, Glyphosate and Atrazine. Drug Chem Toxicol. 2014;37(4):370-7. PubMed PMID: 24328755.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Induction of micronuclei and nuclear lesions in Channa punctatus following exposure to carbosulfan, glyphosate and atrazine. AU - Nwani,Christopher Didigwu, AU - Nagpure,Naresh Sahebrao, AU - Kumar,Ravindra, AU - Kushwaha,Basdeo, AU - Kumar,Pavan, AU - Lakra,Wazir Singh, Y1 - 2013/12/11/ PY - 2013/12/17/entrez PY - 2013/12/18/pubmed PY - 2015/5/28/medline KW - Atrazine KW - Channa punctatus KW - carbosulfan KW - genotoxicity KW - glyphosate KW - micronucleus test KW - nuclear lesions SP - 370 EP - 7 JF - Drug and chemical toxicology JO - Drug Chem Toxicol VL - 37 IS - 4 N2 - The genotoxic effects of commonly used agricultural pesticides viz., carbosulfan, glyphosate, and atrazine, were evaluated in Channa punctatus (Pisces, Perciformes) using micronucleus (MN) test and induction of nuclear lesions (NL). The 96 h LC50 value were estimated by probit analysis as 0.27, 32.0 and 42.0 mg L(-1), respectively, for carbosulfan, glyphosate, and atrazine using semi-static bioassays. Based on these values, three sublethal test concentrations of carbosulfan (0.07, 0.13, 0.20 mg L(-1)), glyphosate (8.1, 16.3, 24.4 mg L(-1)) and atrazine (10.6, 21.2, 31.8 mg L(-1)) corresponding to ¼, ½ and ¾ of the LC50 of the pesticides respectively, were selected for exposure for 96 h. Peripheral blood samplings were taken at intervals of 24 h for assessment of MN and NL frequencies. Considerably higher genotoxic damage was induced by carbosulfan as compared to glyphosate and atrazine. There were significant effects (p < 0.01) of concentrations in all the treated groups. The induction of MN and NL was highest at 96 h pesticide exposure at all test concentrations. The nuclear abnormalities recorded in this study, such as blebbed-, lobed-, notched- and bi-nuclei, other than micronuclei, are indicators of genotoxic damage. SN - 1525-6014 UR - https://www.unboundmedicine.com/medline/citation/24328755/Induction_of_micronuclei_and_nuclear_lesions_in_Channa_punctatus_following_exposure_to_carbosulfan_glyphosate_and_atrazine_ L2 - https://www.tandfonline.com/doi/full/10.3109/01480545.2013.866138 DB - PRIME DP - Unbound Medicine ER -