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High-energy collision-induced dissociation tandem mass spectrometry of regioisomeric lactose palmitic acid monoesters using matrix-assisted laser desorption/ionization.
Rapid Commun Mass Spectrom. 2014 Jan 30; 28(2):169-77.RC

Abstract

RATIONALE

Structural characterization and differentiation of three newly synthesized lactose monopalmitate regioisomers at positions O-3, O-3' and O-6' were realized by single-stage matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry (MALDI-TOF/TOF-MS) in the positive ion mode and by high-energy collision-induced dissociation tandem mass spectrometry (CID-MS/MS).

METHODS

A MALDI-TOF/TOF analyzer was utilized for the analysis of these isobaric lactose monopalmitate regioisomers. The CID-MS/MS spectra were acquired using high-energy cid with a 2 kV potential difference between the source acceleration voltage and the collision cell.

RESULTS

High-energy (CID) tandem mass spectrometry (MS/MS) analyses of the sodiated molecules, [M + Na](+), showed distinguishing cross-ring product ions and characteristic fingerprint product ions, which allowed the straight-forward mass spectrometric characterization of these different regiosiomers.

CONCLUSIONS

This investigation allowed us to unravel the novel fragmentation behavior of the sodiated regioisoimer molecules obtained from the mono-substituted D-lactose fatty acid esters using high-energy CID-TOF/TOF-MS/MS analyses. The high-energy CID of the [M + Na](+) ions from the isobaric lactose monopalmitate regioiosmers promoted the formation of characteristic (0,2) A2 cross-ring cleavage product ions.

Authors+Show Affiliations

Department of Chemistry, Memorial University of Newfoundland, St. John's, Newfoundland, A1B 3V6, Canada; Department of Chemistry, Faculty of Science, Damanhour University, Damanhour, Egypt.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24338964

Citation

Narouz, Mina R., et al. "High-energy Collision-induced Dissociation Tandem Mass Spectrometry of Regioisomeric Lactose Palmitic Acid Monoesters Using Matrix-assisted Laser Desorption/ionization." Rapid Communications in Mass Spectrometry : RCM, vol. 28, no. 2, 2014, pp. 169-77.
Narouz MR, Soliman SE, Fridgen TD, et al. High-energy collision-induced dissociation tandem mass spectrometry of regioisomeric lactose palmitic acid monoesters using matrix-assisted laser desorption/ionization. Rapid Commun Mass Spectrom. 2014;28(2):169-77.
Narouz, M. R., Soliman, S. E., Fridgen, T. D., Nashed, M. A., & Banoub, J. H. (2014). High-energy collision-induced dissociation tandem mass spectrometry of regioisomeric lactose palmitic acid monoesters using matrix-assisted laser desorption/ionization. Rapid Communications in Mass Spectrometry : RCM, 28(2), 169-77. https://doi.org/10.1002/rcm.6770
Narouz MR, et al. High-energy Collision-induced Dissociation Tandem Mass Spectrometry of Regioisomeric Lactose Palmitic Acid Monoesters Using Matrix-assisted Laser Desorption/ionization. Rapid Commun Mass Spectrom. 2014 Jan 30;28(2):169-77. PubMed PMID: 24338964.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High-energy collision-induced dissociation tandem mass spectrometry of regioisomeric lactose palmitic acid monoesters using matrix-assisted laser desorption/ionization. AU - Narouz,Mina R, AU - Soliman,Sameh E, AU - Fridgen,Travis D, AU - Nashed,Mina A, AU - Banoub,Joseph H, PY - 2013/08/26/received PY - 2013/10/06/revised PY - 2013/10/18/accepted PY - 2013/12/17/entrez PY - 2013/12/18/pubmed PY - 2014/7/16/medline SP - 169 EP - 77 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 28 IS - 2 N2 - RATIONALE: Structural characterization and differentiation of three newly synthesized lactose monopalmitate regioisomers at positions O-3, O-3' and O-6' were realized by single-stage matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry (MALDI-TOF/TOF-MS) in the positive ion mode and by high-energy collision-induced dissociation tandem mass spectrometry (CID-MS/MS). METHODS: A MALDI-TOF/TOF analyzer was utilized for the analysis of these isobaric lactose monopalmitate regioisomers. The CID-MS/MS spectra were acquired using high-energy cid with a 2 kV potential difference between the source acceleration voltage and the collision cell. RESULTS: High-energy (CID) tandem mass spectrometry (MS/MS) analyses of the sodiated molecules, [M + Na](+), showed distinguishing cross-ring product ions and characteristic fingerprint product ions, which allowed the straight-forward mass spectrometric characterization of these different regiosiomers. CONCLUSIONS: This investigation allowed us to unravel the novel fragmentation behavior of the sodiated regioisoimer molecules obtained from the mono-substituted D-lactose fatty acid esters using high-energy CID-TOF/TOF-MS/MS analyses. The high-energy CID of the [M + Na](+) ions from the isobaric lactose monopalmitate regioiosmers promoted the formation of characteristic (0,2) A2 cross-ring cleavage product ions. SN - 1097-0231 UR - https://www.unboundmedicine.com/medline/citation/24338964/High_energy_collision_induced_dissociation_tandem_mass_spectrometry_of_regioisomeric_lactose_palmitic_acid_monoesters_using_matrix_assisted_laser_desorption/ionization_ L2 - https://doi.org/10.1002/rcm.6770 DB - PRIME DP - Unbound Medicine ER -