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Simultaneous detection of green tea catechins and gallic acid in human serum after ingestion of green tea tablets using ion-pair high-performance liquid chromatography with electrochemical detection.
J Chromatogr B Analyt Technol Biomed Life Sci. 2014 Jan 15; 945-946:147-53.JC

Abstract

We developed an analytical method for the simultaneous determination of tea catechins and gallic acid (GA) in human serum using ion-pair high-performance liquid chromatography (HPLC) with electrochemical detection. GA was measured to estimate the amount of gallate moiety produced by degradation of gallated catechins ((-)-epicatechin-3-gallate, ECG; (-)-epigallocatechin-3-gallate, EGCG). Ethyl gallate was adopted as an internal standard to correct for the extraction efficiency. To maximize extraction efficiency, a hydrophobic polytetrafluoroethylene (PTFE) filter was selected for pre-treatment prior to separation. HPLC separation was performed using a C18 reversed-phase column with a gradient mobile phase of phosphate buffer (pH 2.5) containing tetrahexylammonium hydrogensulfate as an ion-pair reagent. Using this method, (-)-epicatechin (EC), (-)-epigallocatechin (EGC), ECG, EGCG, ethyl gallate, and GA were detected as single peaks. The resolution values for target analytes were 4.0-13.0 and the mean values of the absolute recoveries of catechins and GA were 77.3-93.9%. The detection limits for catechins and GA in serum were 0.4-3.1ng/mL. The serum catechin levels of eight healthy volunteers after ingestion of a single dose of green tea tablets were measured using this method. The concentration of total catechins (free+conjugated forms) in serum peaked 60min after ingestion. From these results, this method is thought to enable the simultaneous quantification of GA, the hydrolysis product of gallated catechins, and target catechins, and to be sufficiently sensitive for pharmacokinetic studies of catechins following oral administration of green tea.

Authors+Show Affiliations

First Department of Clinical Pharmacy, School of Pharmaceutical Sciences, Kyushu University of Health & Welfare, 1714-1 Yoshino-cho, Nobeoka, Miyazaki 882-8508, Japan.First Department of Clinical Pharmacy, School of Pharmaceutical Sciences, Kyushu University of Health & Welfare, 1714-1 Yoshino-cho, Nobeoka, Miyazaki 882-8508, Japan. Electronic address: son@phoenix.ac.jp.First Department of Clinical Pharmacy, School of Pharmaceutical Sciences, Kyushu University of Health & Welfare, 1714-1 Yoshino-cho, Nobeoka, Miyazaki 882-8508, Japan.First Department of Clinical Pharmacy, School of Pharmaceutical Sciences, Kyushu University of Health & Welfare, 1714-1 Yoshino-cho, Nobeoka, Miyazaki 882-8508, Japan.First Department of Clinical Pharmacy, School of Pharmaceutical Sciences, Kyushu University of Health & Welfare, 1714-1 Yoshino-cho, Nobeoka, Miyazaki 882-8508, Japan.First Department of Clinical Pharmacy, School of Pharmaceutical Sciences, Kyushu University of Health & Welfare, 1714-1 Yoshino-cho, Nobeoka, Miyazaki 882-8508, Japan.First Department of Clinical Pharmacy, School of Pharmaceutical Sciences, Kyushu University of Health & Welfare, 1714-1 Yoshino-cho, Nobeoka, Miyazaki 882-8508, Japan.Department of Clinical Biochemistry, School of Pharmaceutical Sciences, Kyushu University of Health & Welfare, 1714-1 Yoshino-cho, Nobeoka, Miyazaki 882-8508, Japan.First Department of Clinical Pharmacy, School of Pharmaceutical Sciences, Kyushu University of Health & Welfare, 1714-1 Yoshino-cho, Nobeoka, Miyazaki 882-8508, Japan.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24342507

Citation

Narumi, Keiko, et al. "Simultaneous Detection of Green Tea Catechins and Gallic Acid in Human Serum After Ingestion of Green Tea Tablets Using Ion-pair High-performance Liquid Chromatography With Electrochemical Detection." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 945-946, 2014, pp. 147-53.
Narumi K, Sonoda J, Shiotani K, et al. Simultaneous detection of green tea catechins and gallic acid in human serum after ingestion of green tea tablets using ion-pair high-performance liquid chromatography with electrochemical detection. J Chromatogr B Analyt Technol Biomed Life Sci. 2014;945-946:147-53.
Narumi, K., Sonoda, J., Shiotani, K., Shigeru, M., Shibata, M., Kawachi, A., Tomishige, E., Sato, K., & Motoya, T. (2014). Simultaneous detection of green tea catechins and gallic acid in human serum after ingestion of green tea tablets using ion-pair high-performance liquid chromatography with electrochemical detection. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 945-946, 147-53. https://doi.org/10.1016/j.jchromb.2013.11.007
Narumi K, et al. Simultaneous Detection of Green Tea Catechins and Gallic Acid in Human Serum After Ingestion of Green Tea Tablets Using Ion-pair High-performance Liquid Chromatography With Electrochemical Detection. J Chromatogr B Analyt Technol Biomed Life Sci. 2014 Jan 15;945-946:147-53. PubMed PMID: 24342507.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous detection of green tea catechins and gallic acid in human serum after ingestion of green tea tablets using ion-pair high-performance liquid chromatography with electrochemical detection. AU - Narumi,Keiko, AU - Sonoda,Jun-Ichiro, AU - Shiotani,Keita, AU - Shigeru,Michihiro, AU - Shibata,Masayuki, AU - Kawachi,Akio, AU - Tomishige,Erisa, AU - Sato,Keizo, AU - Motoya,Toshiro, Y1 - 2013/12/01/ PY - 2013/06/28/received PY - 2013/10/26/revised PY - 2013/11/04/accepted PY - 2013/12/18/entrez PY - 2013/12/18/pubmed PY - 2014/9/4/medline KW - Electrochemical detection KW - Gallic acid KW - Green tea catechins KW - HPLC KW - Ion-pair reagent KW - Pharmacokinetic study SP - 147 EP - 53 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 945-946 N2 - We developed an analytical method for the simultaneous determination of tea catechins and gallic acid (GA) in human serum using ion-pair high-performance liquid chromatography (HPLC) with electrochemical detection. GA was measured to estimate the amount of gallate moiety produced by degradation of gallated catechins ((-)-epicatechin-3-gallate, ECG; (-)-epigallocatechin-3-gallate, EGCG). Ethyl gallate was adopted as an internal standard to correct for the extraction efficiency. To maximize extraction efficiency, a hydrophobic polytetrafluoroethylene (PTFE) filter was selected for pre-treatment prior to separation. HPLC separation was performed using a C18 reversed-phase column with a gradient mobile phase of phosphate buffer (pH 2.5) containing tetrahexylammonium hydrogensulfate as an ion-pair reagent. Using this method, (-)-epicatechin (EC), (-)-epigallocatechin (EGC), ECG, EGCG, ethyl gallate, and GA were detected as single peaks. The resolution values for target analytes were 4.0-13.0 and the mean values of the absolute recoveries of catechins and GA were 77.3-93.9%. The detection limits for catechins and GA in serum were 0.4-3.1ng/mL. The serum catechin levels of eight healthy volunteers after ingestion of a single dose of green tea tablets were measured using this method. The concentration of total catechins (free+conjugated forms) in serum peaked 60min after ingestion. From these results, this method is thought to enable the simultaneous quantification of GA, the hydrolysis product of gallated catechins, and target catechins, and to be sufficiently sensitive for pharmacokinetic studies of catechins following oral administration of green tea. SN - 1873-376X UR - https://www.unboundmedicine.com/medline/citation/24342507/Simultaneous_detection_of_green_tea_catechins_and_gallic_acid_in_human_serum_after_ingestion_of_green_tea_tablets_using_ion_pair_high_performance_liquid_chromatography_with_electrochemical_detection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(13)00620-X DB - PRIME DP - Unbound Medicine ER -