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Activities of the RNAI and RNAII promoters of plasmid pBR322.
J Bacteriol 1987; 169(3):1217-22JB

Abstract

The synthesis rates of the replication control RNAs of plasmid pBR322, RNAI, an inhibitor of replication, and RNAII, the preprimer, have been determined by hybridizing in vivo pulse-labeled RNA to specific, single-stranded DNA probes for RNAI and RNAII. In Escherichia coli growing in glycerol minimal medium, RNAI transcripts were made at a rate of one molecule per 30 s per plasmid; RNAII was transcribed fivefold less, at a rate of one molecule per 3 min per plasmid. It is estimated that only 1 in 20 prepriming events leads to replication.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

2434459

Citation

Lin-Chao, S, and H Bremer. "Activities of the RNAI and RNAII Promoters of Plasmid PBR322." Journal of Bacteriology, vol. 169, no. 3, 1987, pp. 1217-22.
Lin-Chao S, Bremer H. Activities of the RNAI and RNAII promoters of plasmid pBR322. J Bacteriol. 1987;169(3):1217-22.
Lin-Chao, S., & Bremer, H. (1987). Activities of the RNAI and RNAII promoters of plasmid pBR322. Journal of Bacteriology, 169(3), pp. 1217-22.
Lin-Chao S, Bremer H. Activities of the RNAI and RNAII Promoters of Plasmid PBR322. J Bacteriol. 1987;169(3):1217-22. PubMed PMID: 2434459.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Activities of the RNAI and RNAII promoters of plasmid pBR322. AU - Lin-Chao,S, AU - Bremer,H, PY - 1987/3/1/pubmed PY - 1987/3/1/medline PY - 1987/3/1/entrez SP - 1217 EP - 22 JF - Journal of bacteriology JO - J. Bacteriol. VL - 169 IS - 3 N2 - The synthesis rates of the replication control RNAs of plasmid pBR322, RNAI, an inhibitor of replication, and RNAII, the preprimer, have been determined by hybridizing in vivo pulse-labeled RNA to specific, single-stranded DNA probes for RNAI and RNAII. In Escherichia coli growing in glycerol minimal medium, RNAI transcripts were made at a rate of one molecule per 30 s per plasmid; RNAII was transcribed fivefold less, at a rate of one molecule per 3 min per plasmid. It is estimated that only 1 in 20 prepriming events leads to replication. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/2434459/Activities_of_the_RNAI_and_RNAII_promoters_of_plasmid_pBR322_ L2 - http://jb.asm.org/cgi/pmidlookup?view=long&pmid=2434459 DB - PRIME DP - Unbound Medicine ER -