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Qualitative and quantitative analysis of flavonoids in Sophora tonkinensis by LC/MS and HPLC.
Chin J Nat Med. 2013 Nov; 11(6):690-8.CJ

Abstract

AIM

To develop analytical methods for the identification and determination of the flavonoids in Sophora tonkinensis for comprehensive quality evaluation.

METHODS

An HPLC-DAD-ESI-MS method was used for the separation and characterization of the flavonoids in S. tonkinensis, and a liquid chromatographic method was employed to simultaneously determine five major active flavonoids in this crude drug.

RESULTS

Seventeen flavonoids were identified, among which, seven were unambiguously identified as trifolirhizin, quercetin, formononetin, macckiain, kurarinone, sophoranone, and sophoranochromene by comparing their retention times, and UV and MS spectra with those of the authentic compounds, and the other ten flavonoids were tentatively identified by comparing their UV and MS/MS spectra with those of literature data. Furthermore, five major active flavonoids, including trifolirhizin, quercetin, maackiain, sophoranone, and sophoranochromene were determined in S. tonkinensis. All calibration curves expressed good linearity (r > 0.999 8) within the test ranges, and the recovery from this method was 96.40%-104.43%. The developed HPLC method was successfully applied to quantitatively determine the five flavonoids in seventeen samples of S. tonkinensis.

CONCLUSION

The developed method rapidly characterized the bioactive flavonoids of S. tonkinensis, and could be readily utilized to enhance the quality assurance approaches for this traditional Chinese medicine.

Authors+Show Affiliations

Department of Pharmacognosy, School of Pharmacy, Fudan University, Shanghai 201203, China.Department of Pharmacognosy, School of Pharmacy, Fudan University, Shanghai 201203, China.Department of Pharmacognosy, School of Pharmacy, Fudan University, Shanghai 201203, China. Electronic address: dfchen@shmu.edu.cn.

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24345512

Citation

He, Chang-Ming, et al. "Qualitative and Quantitative Analysis of Flavonoids in Sophora Tonkinensis By LC/MS and HPLC." Chinese Journal of Natural Medicines, vol. 11, no. 6, 2013, pp. 690-8.
He CM, Cheng ZH, Chen DF. Qualitative and quantitative analysis of flavonoids in Sophora tonkinensis by LC/MS and HPLC. Chin J Nat Med. 2013;11(6):690-8.
He, C. M., Cheng, Z. H., & Chen, D. F. (2013). Qualitative and quantitative analysis of flavonoids in Sophora tonkinensis by LC/MS and HPLC. Chinese Journal of Natural Medicines, 11(6), 690-8. https://doi.org/10.1016/S1875-5364(13)60081-3
He CM, Cheng ZH, Chen DF. Qualitative and Quantitative Analysis of Flavonoids in Sophora Tonkinensis By LC/MS and HPLC. Chin J Nat Med. 2013;11(6):690-8. PubMed PMID: 24345512.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Qualitative and quantitative analysis of flavonoids in Sophora tonkinensis by LC/MS and HPLC. AU - He,Chang-Ming, AU - Cheng,Zhi-Hong, AU - Chen,Dao-Feng, PY - 2013/02/28/received PY - 2013/12/19/entrez PY - 2013/12/19/pubmed PY - 2014/7/30/medline KW - Flavonoids KW - HPLC KW - Identification KW - LC/MS KW - Quantification KW - Sophora tonkinensis SP - 690 EP - 8 JF - Chinese journal of natural medicines JO - Chin J Nat Med VL - 11 IS - 6 N2 - AIM: To develop analytical methods for the identification and determination of the flavonoids in Sophora tonkinensis for comprehensive quality evaluation. METHODS: An HPLC-DAD-ESI-MS method was used for the separation and characterization of the flavonoids in S. tonkinensis, and a liquid chromatographic method was employed to simultaneously determine five major active flavonoids in this crude drug. RESULTS: Seventeen flavonoids were identified, among which, seven were unambiguously identified as trifolirhizin, quercetin, formononetin, macckiain, kurarinone, sophoranone, and sophoranochromene by comparing their retention times, and UV and MS spectra with those of the authentic compounds, and the other ten flavonoids were tentatively identified by comparing their UV and MS/MS spectra with those of literature data. Furthermore, five major active flavonoids, including trifolirhizin, quercetin, maackiain, sophoranone, and sophoranochromene were determined in S. tonkinensis. All calibration curves expressed good linearity (r > 0.999 8) within the test ranges, and the recovery from this method was 96.40%-104.43%. The developed HPLC method was successfully applied to quantitatively determine the five flavonoids in seventeen samples of S. tonkinensis. CONCLUSION: The developed method rapidly characterized the bioactive flavonoids of S. tonkinensis, and could be readily utilized to enhance the quality assurance approaches for this traditional Chinese medicine. SN - 1875-5364 UR - https://www.unboundmedicine.com/medline/citation/24345512/Qualitative_and_quantitative_analysis_of_flavonoids_in_Sophora_tonkinensis_by_LC/MS_and_HPLC_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1875-5364(13)60081-3 DB - PRIME DP - Unbound Medicine ER -