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Isolation and phylogenetic analysis of an orf virus from sheep in Makhdoom, India.
Virus Genes 2014; 48(2):312-9VG

Abstract

Orf (contagious ecthyma) is an exanthematic disease caused by a parapoxvirus and occurs primarily in sheep and goats with zoonotic implications. In the present investigation, an orf outbreak in the Muzzaffarnagari sheep flock at the Central Institute for Research on Goats (CIRG), Makhdoom, Mathura, Uttar Pradesh, India, was investigated. Primary goat testes cell culture was used for isolation of the orf virus (ORFV) for the first time. The identity of the virus was confirmed by amplification and sequence analysis of the major envelope glycoprotein (B2L) gene and named ORFV/sheep/India/2012/CIRG. On phylogenetic analysis of B2L protein gene, it clustered with the ORFV strains from China suggesting distinct ORFV strains are circulating in India. On comparison of nucleotide and deduced amino acid sequence analysis (n = 63), a unique 126S residue was observed in ORFV/sheep/India/2012/CIRG. On further sequence analysis (B2L) of different ORFV strains (n = 63), some conserved amino acid residues were identified as host-specific (sheep, human, camel, takin, and musk ox) and have been summarized.

Authors+Show Affiliations

Virology Laboratory, Division of Animal Health, Central Institute for Research on Goats, Indian Council of Agricultural Research, Makhdoom, P.O.-Farah, Mathura, UP, 281122, India, naveenkumar.icar@gmail.com.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24347045

Citation

Kumar, Naveen, et al. "Isolation and Phylogenetic Analysis of an Orf Virus From Sheep in Makhdoom, India." Virus Genes, vol. 48, no. 2, 2014, pp. 312-9.
Kumar N, Wadhwa A, Chaubey KK, et al. Isolation and phylogenetic analysis of an orf virus from sheep in Makhdoom, India. Virus Genes. 2014;48(2):312-9.
Kumar, N., Wadhwa, A., Chaubey, K. K., Singh, S. V., Gupta, S., Sharma, S., ... Mishra, A. K. (2014). Isolation and phylogenetic analysis of an orf virus from sheep in Makhdoom, India. Virus Genes, 48(2), pp. 312-9. doi:10.1007/s11262-013-1025-9.
Kumar N, et al. Isolation and Phylogenetic Analysis of an Orf Virus From Sheep in Makhdoom, India. Virus Genes. 2014;48(2):312-9. PubMed PMID: 24347045.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation and phylogenetic analysis of an orf virus from sheep in Makhdoom, India. AU - Kumar,Naveen, AU - Wadhwa,Ashutosh, AU - Chaubey,Kundan K, AU - Singh,Shoor V, AU - Gupta,Sorabh, AU - Sharma,Shalini, AU - Sharma,Dinesh K, AU - Singh,Manoj K, AU - Mishra,A K, Y1 - 2013/12/18/ PY - 2013/09/25/received PY - 2013/12/10/accepted PY - 2013/12/19/entrez PY - 2013/12/19/pubmed PY - 2014/12/15/medline SP - 312 EP - 9 JF - Virus genes JO - Virus Genes VL - 48 IS - 2 N2 - Orf (contagious ecthyma) is an exanthematic disease caused by a parapoxvirus and occurs primarily in sheep and goats with zoonotic implications. In the present investigation, an orf outbreak in the Muzzaffarnagari sheep flock at the Central Institute for Research on Goats (CIRG), Makhdoom, Mathura, Uttar Pradesh, India, was investigated. Primary goat testes cell culture was used for isolation of the orf virus (ORFV) for the first time. The identity of the virus was confirmed by amplification and sequence analysis of the major envelope glycoprotein (B2L) gene and named ORFV/sheep/India/2012/CIRG. On phylogenetic analysis of B2L protein gene, it clustered with the ORFV strains from China suggesting distinct ORFV strains are circulating in India. On comparison of nucleotide and deduced amino acid sequence analysis (n = 63), a unique 126S residue was observed in ORFV/sheep/India/2012/CIRG. On further sequence analysis (B2L) of different ORFV strains (n = 63), some conserved amino acid residues were identified as host-specific (sheep, human, camel, takin, and musk ox) and have been summarized. SN - 1572-994X UR - https://www.unboundmedicine.com/medline/citation/24347045/Isolation_and_phylogenetic_analysis_of_an_orf_virus_from_sheep_in_Makhdoom_India_ L2 - https://doi.org/10.1007/s11262-013-1025-9 DB - PRIME DP - Unbound Medicine ER -