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Evaluation of in vitro antioxidant properties of methanol and aqueous extracts of Parkinsonia aculeata L. leaves.
ScientificWorldJournal. 2013; 2013:604865.S

Abstract

In the present study, methanol and aqueous extracts of Parkinsonia aculeata L. leaves were prepared and analyzed for phytochemical analysis and antioxidant potential in different in vitro assays. Antioxidant activity was studied using DPPH, CUPRAC, reducing power assay, deoxyribose degradation (site and nonsite specific), ferric reducing antioxidant potential (FRAP), ferric thiocyanate (FTC), thiobarbituric acid (TBA), and molybdate ion reduction, respectively. The total phenolic contents of the methanol and aqueous leaf extract were 39 mg GAE/g and 38 mg GAE/g, whereas flavonoid contents of these extracts were found to be 0.013 mg RE/g and 0.006 mg RE/g, respectively. From the two extracts, the methanol extract shows maximum inhibition (%) of 57.82%, 71.23%, 48.26%, 69.85%, and 52.78% in DPPH, nonsite- and site-specific, FTC, and TBA assays and absorbance of 0.669 and 0.241 in reducing power and CUPRAC assays at the highest concentration tested. UPLC analysis was done to determine the presence of various types of polyphenols present in plant extracts.

Authors+Show Affiliations

Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar, Punjab 143005, India.Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar, Punjab 143005, India.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24348173

Citation

Sharma, Sonia, and Adarsh Pal Vig. "Evaluation of in Vitro Antioxidant Properties of Methanol and Aqueous Extracts of Parkinsonia Aculeata L. Leaves." TheScientificWorldJournal, vol. 2013, 2013, p. 604865.
Sharma S, Vig AP. Evaluation of in vitro antioxidant properties of methanol and aqueous extracts of Parkinsonia aculeata L. leaves. ScientificWorldJournal. 2013;2013:604865.
Sharma, S., & Vig, A. P. (2013). Evaluation of in vitro antioxidant properties of methanol and aqueous extracts of Parkinsonia aculeata L. leaves. TheScientificWorldJournal, 2013, 604865. https://doi.org/10.1155/2013/604865
Sharma S, Vig AP. Evaluation of in Vitro Antioxidant Properties of Methanol and Aqueous Extracts of Parkinsonia Aculeata L. Leaves. ScientificWorldJournal. 2013;2013:604865. PubMed PMID: 24348173.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of in vitro antioxidant properties of methanol and aqueous extracts of Parkinsonia aculeata L. leaves. AU - Sharma,Sonia, AU - Vig,Adarsh Pal, Y1 - 2013/11/14/ PY - 2013/08/05/received PY - 2013/09/18/accepted PY - 2013/12/19/entrez PY - 2013/12/19/pubmed PY - 2014/9/13/medline SP - 604865 EP - 604865 JF - TheScientificWorldJournal JO - ScientificWorldJournal VL - 2013 N2 - In the present study, methanol and aqueous extracts of Parkinsonia aculeata L. leaves were prepared and analyzed for phytochemical analysis and antioxidant potential in different in vitro assays. Antioxidant activity was studied using DPPH, CUPRAC, reducing power assay, deoxyribose degradation (site and nonsite specific), ferric reducing antioxidant potential (FRAP), ferric thiocyanate (FTC), thiobarbituric acid (TBA), and molybdate ion reduction, respectively. The total phenolic contents of the methanol and aqueous leaf extract were 39 mg GAE/g and 38 mg GAE/g, whereas flavonoid contents of these extracts were found to be 0.013 mg RE/g and 0.006 mg RE/g, respectively. From the two extracts, the methanol extract shows maximum inhibition (%) of 57.82%, 71.23%, 48.26%, 69.85%, and 52.78% in DPPH, nonsite- and site-specific, FTC, and TBA assays and absorbance of 0.669 and 0.241 in reducing power and CUPRAC assays at the highest concentration tested. UPLC analysis was done to determine the presence of various types of polyphenols present in plant extracts. SN - 1537-744X UR - https://www.unboundmedicine.com/medline/citation/24348173/Evaluation_of_in_vitro_antioxidant_properties_of_methanol_and_aqueous_extracts_of_Parkinsonia_aculeata_L__leaves_ DB - PRIME DP - Unbound Medicine ER -
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