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Therapeutic potential of anti-IL-1β IgY in guinea pigs with allergic asthma induced by ovalbumin.
Mol Immunol. 2014 Mar; 58(1):139-49.MI

Abstract

BACKGROUND

Interleukin-1 beta (IL-1β) plays pivotal roles in the progression of allergic airway inflammation. This study aims to determine whether the blockade of IL-1β can inhibit airway inflammation in guinea pigs with allergic asthma induced by the inhalation of aerosolized ovalbumin (OVA).

METHODS

Healthy guinea pigs treated with saline were used as normal controls (group C). The guinea pigs with allergic asthma induced by the inhalation of aerosolized OVA were randomly divided into three groups: (1) the M group containing negative control animals treated with saline; (2) the Z1 group containing animals treated by the inhalation of atomized 0.1% anti-IL-1β immunoglobulin yolk (IgY); and (3) the Z2 group containing positive control animals that were treated with budesonide. The inflammatory cells in the peripheral blood (PB) and bronchoalveolar lavage fluid (BALF) were evaluated using methylene blue and eosin staining. Cytokine concentrations were measured using an enzyme-linked immunosorbent assay. Pulmonary sections were examined using hematoxylin-eosin staining.

RESULTS

Allergic inflammation and damage to the pulmonary tissues were decreased in the Z1 group compared to the M group. Eosinophils and neutrophils in the PB and BALF were significantly decreased in the Z1 group compared to the M group (P<0.05). Treatment with anti-IL-1β IgY significantly reduced the levels of IL-1β, IL-4, IL-8, IL-13, TNF-α, TGF-β1 and IgE in the BALF (P<0.05).

CONCLUSION

The inhalation of aerosolized anti-IL-1β IgY inhibits pathological responses in the pulmonary tissues of guinea pigs with allergic asthma. The inhibitory activity may be due to the decrease in the numbers of eosinophils and neutrophils and the reduced levels of inflammatory cytokines and IgE in the PB and BALF.

Authors+Show Affiliations

Department of Radiation Oncology, Zhongshan Hospital, Fudan University, Shanghai 200032, China.Institute of Clinical Medical Sciences, Jiangxi Province People's Hospital, Nanchang 330006, China.Department of Hematology, Jiangxi Academy of Medical Science, Nanchang 330006, China.Institute of Clinical Medical Sciences, Jiangxi Province People's Hospital, Nanchang 330006, China.Institute of Clinical Medical Sciences, Jiangxi Province People's Hospital, Nanchang 330006, China.Department of Experimental Animal, Institute of Occupational Medicine of Jiangxi, Nanchang 330006, China.Institute of Clinical Medical Sciences, Jiangxi Province People's Hospital, Nanchang 330006, China.Institute of Clinical Medical Sciences, Jiangxi Province People's Hospital, Nanchang 330006, China.Institute of Clinical Medical Sciences, Jiangxi Province People's Hospital, Nanchang 330006, China. Electronic address: hgz56@126.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24355520

Citation

Wei-xu, Hu, et al. "Therapeutic Potential of anti-IL-1β IgY in Guinea Pigs With Allergic Asthma Induced By Ovalbumin." Molecular Immunology, vol. 58, no. 1, 2014, pp. 139-49.
Wei-xu H, Qin X, Zhu W, et al. Therapeutic potential of anti-IL-1β IgY in guinea pigs with allergic asthma induced by ovalbumin. Mol Immunol. 2014;58(1):139-49.
Wei-xu, H., Qin, X., Zhu, W., Yuan-yi, C., Li-feng, Z., Zhi-yong, L., Dan, H., Xiao-mu, W., & Guo-zhu, H. (2014). Therapeutic potential of anti-IL-1β IgY in guinea pigs with allergic asthma induced by ovalbumin. Molecular Immunology, 58(1), 139-49. https://doi.org/10.1016/j.molimm.2013.11.006
Wei-xu H, et al. Therapeutic Potential of anti-IL-1β IgY in Guinea Pigs With Allergic Asthma Induced By Ovalbumin. Mol Immunol. 2014;58(1):139-49. PubMed PMID: 24355520.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Therapeutic potential of anti-IL-1β IgY in guinea pigs with allergic asthma induced by ovalbumin. AU - Wei-xu,Hu, AU - Qin,Xiang, AU - Zhu,Wen, AU - Yuan-yi,Chen, AU - Li-feng,Zeng, AU - Zhi-yong,Liu, AU - Dan,He, AU - Xiao-mu,Wu, AU - Guo-zhu,Hu, Y1 - 2013/12/24/ PY - 2013/5/4/received PY - 2013/11/8/revised PY - 2013/11/9/accepted PY - 2013/12/21/entrez PY - 2013/12/21/pubmed PY - 2014/7/10/medline KW - AHR KW - Allergic asthma KW - BALF KW - Cytokines KW - Guinea pigs KW - HE KW - IL-1 receptor antagonist KW - IL-13 KW - IL-1ra KW - IL-1β KW - IL-4 KW - IL-8 KW - IgE KW - IgY KW - Immunoglobulin yolk KW - Infliximab KW - Interleukin-1β KW - OVA KW - Omalizumab KW - PB KW - T helper type 2 KW - TGF-β(1) KW - TNF-α KW - Th2 KW - airway hyperresponsiveness KW - anti-IgE antibody KW - anti-TNF-α antibody KW - bronchoalveolar lavage fluid KW - hematoxylin-eosin KW - immunoglobulin E KW - immunoglobulin yolk KW - interleukin-13 KW - interleukin-1beta KW - interleukin-4 KW - interleukin-8 KW - ovalbumin KW - peripheral blood KW - sRaw KW - sTNFR KW - soluble TNF-α receptor KW - specific airway resistance KW - transforming growth factor-beta 1 KW - tumor necrosis factor-alpha SP - 139 EP - 49 JF - Molecular immunology JO - Mol Immunol VL - 58 IS - 1 N2 - BACKGROUND: Interleukin-1 beta (IL-1β) plays pivotal roles in the progression of allergic airway inflammation. This study aims to determine whether the blockade of IL-1β can inhibit airway inflammation in guinea pigs with allergic asthma induced by the inhalation of aerosolized ovalbumin (OVA). METHODS: Healthy guinea pigs treated with saline were used as normal controls (group C). The guinea pigs with allergic asthma induced by the inhalation of aerosolized OVA were randomly divided into three groups: (1) the M group containing negative control animals treated with saline; (2) the Z1 group containing animals treated by the inhalation of atomized 0.1% anti-IL-1β immunoglobulin yolk (IgY); and (3) the Z2 group containing positive control animals that were treated with budesonide. The inflammatory cells in the peripheral blood (PB) and bronchoalveolar lavage fluid (BALF) were evaluated using methylene blue and eosin staining. Cytokine concentrations were measured using an enzyme-linked immunosorbent assay. Pulmonary sections were examined using hematoxylin-eosin staining. RESULTS: Allergic inflammation and damage to the pulmonary tissues were decreased in the Z1 group compared to the M group. Eosinophils and neutrophils in the PB and BALF were significantly decreased in the Z1 group compared to the M group (P<0.05). Treatment with anti-IL-1β IgY significantly reduced the levels of IL-1β, IL-4, IL-8, IL-13, TNF-α, TGF-β1 and IgE in the BALF (P<0.05). CONCLUSION: The inhalation of aerosolized anti-IL-1β IgY inhibits pathological responses in the pulmonary tissues of guinea pigs with allergic asthma. The inhibitory activity may be due to the decrease in the numbers of eosinophils and neutrophils and the reduced levels of inflammatory cytokines and IgE in the PB and BALF. SN - 1872-9142 UR - https://www.unboundmedicine.com/medline/citation/24355520/Therapeutic_potential_of_anti_IL_1β_IgY_in_guinea_pigs_with_allergic_asthma_induced_by_ovalbumin_ DB - PRIME DP - Unbound Medicine ER -