TY - JOUR T1 - The use of coenzyme Q0 as a template in the development of a molecularly imprinted polymer for the selective recognition of coenzyme Q10. AU - Contin,Mario, AU - Flor,Sabrina, AU - Martinefski,Manuela, AU - Lucangioli,Silvia, AU - Tripodi,Valeria, Y1 - 2013/11/21/ PY - 2013/08/28/received PY - 2013/11/12/revised PY - 2013/11/14/accepted PY - 2013/12/21/entrez PY - 2013/12/21/pubmed PY - 2014/8/13/medline KW - ATP KW - BET KW - Biological sample KW - Brunauer–Emmett–Teller KW - CoQ0 KW - CoQ10 KW - Coenzyme Q10 KW - ECD KW - EGDMA KW - FEG-SEM KW - HPLC KW - IPB KW - LOD KW - LOQ KW - MAA KW - MIP KW - MISPE KW - MS KW - Molecular imprinting KW - N KW - NIP KW - RSD KW - SEM KW - SPE KW - Solid phase extraction KW - UC KW - adenosine-triphosphate KW - coenzyme Q0 KW - coenzyme Q10 KW - electrochemical detection KW - ethylene glycoldimethacrylate KW - field emission gun scanning electron microscopy KW - high performance liquid chromatography KW - imprinting-induced promotion of binding KW - limit of detection KW - limit of quantification KW - mass spectrometry KW - methacrylic acid KW - molecularly imprinted polymers KW - molecularly imprinted solid phase extraction KW - non-imprinted polymer KW - relative standard deviation KW - scanning electron microscopy KW - solid phase extraction KW - theoretical plates KW - ubicromenol SP - 67 EP - 74 JF - Analytica chimica acta JO - Anal Chim Acta VL - 807 N2 - In this work, a novel molecularly imprinted polymer (MIP) for use as a solid phase extraction sorbent was developed for the determination of coenzyme Q10 (CoQ10) in liver extract. CoQ10 is an essential cofactor in mitochondrial oxidative phosphorylation and a powerful antioxidant agent found in low concentrations in biological samples. This fact and its high hydrophobicity make the analysis of CoQ10 technically challenging. Accordingly, a MIP was synthesised using coenzyme Q0 as the template, methacrylic acid as the functional monomer, acetonitrile as the porogen, ethylene glycol dimethacrylate as the crosslinker and benzoyl peroxide as the initiator. Various parameters affecting the polymer preparation and extraction efficiency were evaluated. Morphological characterisation of the MIP and its proper comparison with C18 as a sorbent in solid phase extraction were performed. The optimal conditions for the molecularly imprinted solid phase extraction (MISPE) consisted of 400 μL of sample mixed with 30 mg of MIP and 600 μL of water to reach the optimum solution loading. The loading was followed by a washing step consisting of 1 mL of a 1-propanol solution (1-propanol:water, 30:70,v/v) and elution with 1 mL of 1-propanol. After clean-up, the CoQ10 in the samples was analysed by high performance liquid chromatography. The extraction recoveries were higher than 73.7% with good precision (3.6-8.3%). The limits of detection and quantification were 2.4 and 7.5 μg g(-1), respectively, and a linear range between 7.5 and 150 μg g(-1) of tissue was achieved. The new MISPE procedure provided a successful clean-up for the determination of CoQ10 in a complex matrix. SN - 1873-4324 UR - https://www.unboundmedicine.com/medline/citation/24356222/The_use_of_coenzyme_Q0_as_a_template_in_the_development_of_a_molecularly_imprinted_polymer_for_the_selective_recognition_of_coenzyme_Q10_ DB - PRIME DP - Unbound Medicine ER -