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Development of liquid chromatography-tandem mass spectrometry method for analysis of polyphenolic compounds in liquid samples of grape juice, green tea and coffee.
Food Chem. 2014 May 01; 150:87-93.FC

Abstract

A simple and fast method for the analysis of a wide range of polyphenolic compounds in juice, tea, and coffee samples was developed using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The method was based on a simple sample preparation "dilute and shoot" approach, and LC-MS/MS quantification using genistein-d4 as an internal standard. The performance of six different syringeless filter devices was tested for sample preparation. The method was evaluated for recoveries of polyphenols at three spiking levels in juice, tea, and coffee samples. The recoveries of the majority of polyphenols were satisfactory (70-120%), but some varied significantly (20-138%) depending on the matrix. NIST Standard Reference Materials (SRM) 3257 Catechin Calibration Solutions and 3255 Camellia sinensis (Green Tea) Extract with certified concentrations of catechin and epicatechin were used for method validation. The measurement accuracy in two SRMs was 71-113%. The method was successfully applied to the analysis of liquid samples of grape juice, green tea, and coffee.

Authors+Show Affiliations

US Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, 600 East Mermaid Lane, Wyndmoor, PA 193038, USA. Electronic address: yelena.sapozhnikova@ars.usda.gov.

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

24360423

Citation

Sapozhnikova, Yelena. "Development of Liquid Chromatography-tandem Mass Spectrometry Method for Analysis of Polyphenolic Compounds in Liquid Samples of Grape Juice, Green Tea and Coffee." Food Chemistry, vol. 150, 2014, pp. 87-93.
Sapozhnikova Y. Development of liquid chromatography-tandem mass spectrometry method for analysis of polyphenolic compounds in liquid samples of grape juice, green tea and coffee. Food Chem. 2014;150:87-93.
Sapozhnikova, Y. (2014). Development of liquid chromatography-tandem mass spectrometry method for analysis of polyphenolic compounds in liquid samples of grape juice, green tea and coffee. Food Chemistry, 150, 87-93. https://doi.org/10.1016/j.foodchem.2013.10.131
Sapozhnikova Y. Development of Liquid Chromatography-tandem Mass Spectrometry Method for Analysis of Polyphenolic Compounds in Liquid Samples of Grape Juice, Green Tea and Coffee. Food Chem. 2014 May 1;150:87-93. PubMed PMID: 24360423.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of liquid chromatography-tandem mass spectrometry method for analysis of polyphenolic compounds in liquid samples of grape juice, green tea and coffee. A1 - Sapozhnikova,Yelena, Y1 - 2013/11/04/ PY - 2013/04/25/received PY - 2013/09/17/revised PY - 2013/10/26/accepted PY - 2013/12/24/entrez PY - 2013/12/24/pubmed PY - 2014/8/26/medline KW - Analytical method development KW - Coffee KW - Grape juice KW - LC–MS/MS KW - Polyphenolic compounds KW - Tea SP - 87 EP - 93 JF - Food chemistry JO - Food Chem VL - 150 N2 - A simple and fast method for the analysis of a wide range of polyphenolic compounds in juice, tea, and coffee samples was developed using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The method was based on a simple sample preparation "dilute and shoot" approach, and LC-MS/MS quantification using genistein-d4 as an internal standard. The performance of six different syringeless filter devices was tested for sample preparation. The method was evaluated for recoveries of polyphenols at three spiking levels in juice, tea, and coffee samples. The recoveries of the majority of polyphenols were satisfactory (70-120%), but some varied significantly (20-138%) depending on the matrix. NIST Standard Reference Materials (SRM) 3257 Catechin Calibration Solutions and 3255 Camellia sinensis (Green Tea) Extract with certified concentrations of catechin and epicatechin were used for method validation. The measurement accuracy in two SRMs was 71-113%. The method was successfully applied to the analysis of liquid samples of grape juice, green tea, and coffee. SN - 1873-7072 UR - https://www.unboundmedicine.com/medline/citation/24360423/Development_of_liquid_chromatography_tandem_mass_spectrometry_method_for_analysis_of_polyphenolic_compounds_in_liquid_samples_of_grape_juice_green_tea_and_coffee_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0308-8146(13)01580-X DB - PRIME DP - Unbound Medicine ER -