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Frequency, characterization, and prognostic analysis of PIK3CA gene mutations in Chinese esophageal squamous cell carcinoma.
Hum Pathol. 2014 Feb; 45(2):352-8.HP

Abstract

PIK3CA gene mutations are found in numerous cancers but correlate differently with prognosis. Although the frequency of PIK3CA gene mutation in esophageal squamous cell carcinoma (ESCC) has been previously studied, a prognostic analysis has not been reported. Ninety-six surgically resected ESCC tissues were collected from Chinese patients and DNA was extracted. Gene mutations in PIK3CA (exons 9 and 20), EGFR (exons 18, 19, 20 and 21), KRAS (exons 2 and 3), and BRAF (exons 11 and 15) were screened using mutant-enriched liquid chip technology. PIK3CA gene mutations were identified in 12 of 96 ESCC cases (12.5%). No mutations were identified in EGFR, KRAS or BRAF genes in this study. Correlations between clinicopathological features and PIK3CA mutation status were analyzed and finally, patient survival information was used to determine the prognostic significance of PIK3CA mutation. Interestingly, the frequency of PIK3CA mutation was higher in female ESCC patients (31.3%, 5/16) than in males (8.8%, 7/80), and higher in patients with non-lymph node metastasis (19.6%, 10/51, P = .013) than in patients with lymph node metastasis (4.4%, 2/45, P = .025). Furthermore, patients with PIK3CA-mutated tumors showed a trend towards favorable overall survival (P = .085) but not disease-free survival (P = .238), suggesting that PIK3CA gene status may be a favorable predictive marker in ESCC patients.

Authors+Show Affiliations

Department of Pathology, Zhongshan Hospital, Fudan University, Shanghai 200032, PR China.Department of Pathology, Zhongshan Hospital, Fudan University, Shanghai 200032, PR China.Innovation Center China, AstraZeneca Global R&D, Zhangjiang Hi-Tech Park, Shanghai 201203, PR China.Innovation Center China, AstraZeneca Global R&D, Zhangjiang Hi-Tech Park, Shanghai 201203, PR China.Department of Thorax, Zhongshan hospital, Fudan University, Shanghai 200032, PR China.Department of Pathology, Zhongshan Hospital, Fudan University, Shanghai 200032, PR China.Department of Pathology, Zhongshan Hospital, Fudan University, Shanghai 200032, PR China.Department of Pathology, Zhongshan Hospital, Fudan University, Shanghai 200032, PR China.Department of Pathology, Zhongshan Hospital, Fudan University, Shanghai 200032, PR China.Department of Thorax, Zhongshan hospital, Fudan University, Shanghai 200032, PR China.Innovation Center China, AstraZeneca Global R&D, Zhangjiang Hi-Tech Park, Shanghai 201203, PR China.Innovation Center China, AstraZeneca Global R&D, Zhangjiang Hi-Tech Park, Shanghai 201203, PR China.Department of Pathology, Zhongshan Hospital, Fudan University, Shanghai 200032, PR China. Electronic address: hou.yingyong@hotmail.com.Department of Thorax, Zhongshan hospital, Fudan University, Shanghai 200032, PR China. Electronic address: tan.lijie@zs-hospital.sh.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24360885

Citation

Hou, Jun, et al. "Frequency, Characterization, and Prognostic Analysis of PIK3CA Gene Mutations in Chinese Esophageal Squamous Cell Carcinoma." Human Pathology, vol. 45, no. 2, 2014, pp. 352-8.
Hou J, Jiang D, Zhang J, et al. Frequency, characterization, and prognostic analysis of PIK3CA gene mutations in Chinese esophageal squamous cell carcinoma. Hum Pathol. 2014;45(2):352-8.
Hou, J., Jiang, D., Zhang, J., Gavine, P. R., Xu, S., Liu, Y., Xu, C., Huang, J., Tan, Y., Wang, H., Lu, Y., Zheng, L., Hou, Y., & Tan, L. (2014). Frequency, characterization, and prognostic analysis of PIK3CA gene mutations in Chinese esophageal squamous cell carcinoma. Human Pathology, 45(2), 352-8. https://doi.org/10.1016/j.humpath.2013.09.011
Hou J, et al. Frequency, Characterization, and Prognostic Analysis of PIK3CA Gene Mutations in Chinese Esophageal Squamous Cell Carcinoma. Hum Pathol. 2014;45(2):352-8. PubMed PMID: 24360885.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Frequency, characterization, and prognostic analysis of PIK3CA gene mutations in Chinese esophageal squamous cell carcinoma. AU - Hou,Jun, AU - Jiang,Dongxian, AU - Zhang,Jingchuan, AU - Gavine,Paul R, AU - Xu,Songtao, AU - Liu,Yalan, AU - Xu,Chen, AU - Huang,Jie, AU - Tan,Yunshan, AU - Wang,Hao, AU - Lu,Yachao, AU - Zheng,Li, AU - Hou,Yingyong, AU - Tan,Lijie, Y1 - 2013/10/03/ PY - 2013/07/21/received PY - 2013/09/15/revised PY - 2013/09/18/accepted PY - 2013/12/24/entrez PY - 2013/12/24/pubmed PY - 2014/3/13/medline KW - Esophageal squamous cell carcinoma KW - Mutation KW - PIK3CA gene SP - 352 EP - 8 JF - Human pathology JO - Hum Pathol VL - 45 IS - 2 N2 - PIK3CA gene mutations are found in numerous cancers but correlate differently with prognosis. Although the frequency of PIK3CA gene mutation in esophageal squamous cell carcinoma (ESCC) has been previously studied, a prognostic analysis has not been reported. Ninety-six surgically resected ESCC tissues were collected from Chinese patients and DNA was extracted. Gene mutations in PIK3CA (exons 9 and 20), EGFR (exons 18, 19, 20 and 21), KRAS (exons 2 and 3), and BRAF (exons 11 and 15) were screened using mutant-enriched liquid chip technology. PIK3CA gene mutations were identified in 12 of 96 ESCC cases (12.5%). No mutations were identified in EGFR, KRAS or BRAF genes in this study. Correlations between clinicopathological features and PIK3CA mutation status were analyzed and finally, patient survival information was used to determine the prognostic significance of PIK3CA mutation. Interestingly, the frequency of PIK3CA mutation was higher in female ESCC patients (31.3%, 5/16) than in males (8.8%, 7/80), and higher in patients with non-lymph node metastasis (19.6%, 10/51, P = .013) than in patients with lymph node metastasis (4.4%, 2/45, P = .025). Furthermore, patients with PIK3CA-mutated tumors showed a trend towards favorable overall survival (P = .085) but not disease-free survival (P = .238), suggesting that PIK3CA gene status may be a favorable predictive marker in ESCC patients. SN - 1532-8392 UR - https://www.unboundmedicine.com/medline/citation/24360885/Frequency_characterization_and_prognostic_analysis_of_PIK3CA_gene_mutations_in_Chinese_esophageal_squamous_cell_carcinoma_ L2 - https://www.clinicalkey.com/content/playBy/pii?v=S0046-8177(13)00408-5 DB - PRIME DP - Unbound Medicine ER -