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Fructose compared with glucose is more a potent glycoxidation agent in vitro, but not under carbohydrate-induced stress in vivo: potential role of antioxidant and antiglycation enzymes.
Carbohydr Res. 2014 Jan 30; 384:61-9.CR

Abstract

The contribution of carbohydrates to non-enzymatic processes such as glycation/autoxidation has been extensively investigated over the last decades. This may be attributed to either beneficial or detrimental effects of reducing carbohydrates, and most studies in the field of glycoxidation are focused on glucose. Non-enzymatic reactions of fructose have not been as thoroughly investigated as those of glucose. To compare glucose and fructose involvement in the generation of glycoxidation products under experimental conditions close to the physiological situation, we used intact Saccharomyces cerevisiae cells as in vivo model and cell-free extracts prepared from whole yeast cells as in vitro model. Both intact cells and cell-free extracts were incubated with glucose or fructose. It was shown that: (i) in vitro fructose was more reactive than glucose and produced higher level of autoxidation and glycation products; (ii) no substantive differences were observed for the effect of glucose and fructose on the intracellular level of glycoxidation products, when intact yeast cells were exposed to the high concentration of hexoses; (iii) the activity of defensive enzymes (superoxide dismutase, catalase, glyoxalases, and glutathione reductase) was increased in both glucose- and fructose-stressed yeasts, indicating the development of oxidative/carbonyl stress; (iv) glucose-6-phosphate dehydrogenase activity significantly dropped in yeast exposed to both hexoses, demonstrating its high sensitivity to reactive oxygen and carbonyl species; and (v) fructose more markedly activated glyoxalases than glucose. Involvement of glucose and fructose in the glycoxidation reactions as well as potential role of antioxidant and antiglycation enzymes in yeast protection against glycoxidation are discussed.

Authors+Show Affiliations

Department of Biochemistry and Biotechnology, Vassyl Stefanyk Precarpathian National University, 57 Shevchenko Str., 76025 Ivano-Frankivsk, Ukraine. Electronic address: semchyshyn@pu.if.ua.Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 7 Gronostajowa Str., 31309 Cracow, Poland.Department of Biochemistry and Biotechnology, Vassyl Stefanyk Precarpathian National University, 57 Shevchenko Str., 76025 Ivano-Frankivsk, Ukraine.Department of Biochemistry and Biotechnology, Vassyl Stefanyk Precarpathian National University, 57 Shevchenko Str., 76025 Ivano-Frankivsk, Ukraine.Department of Biochemistry and Biotechnology, Vassyl Stefanyk Precarpathian National University, 57 Shevchenko Str., 76025 Ivano-Frankivsk, Ukraine.

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24361593

Citation

Semchyshyn, Halyna M., et al. "Fructose Compared With Glucose Is More a Potent Glycoxidation Agent in Vitro, but Not Under Carbohydrate-induced Stress in Vivo: Potential Role of Antioxidant and Antiglycation Enzymes." Carbohydrate Research, vol. 384, 2014, pp. 61-9.
Semchyshyn HM, Miedzobrodzki J, Bayliak MM, et al. Fructose compared with glucose is more a potent glycoxidation agent in vitro, but not under carbohydrate-induced stress in vivo: potential role of antioxidant and antiglycation enzymes. Carbohydr Res. 2014;384:61-9.
Semchyshyn, H. M., Miedzobrodzki, J., Bayliak, M. M., Lozinska, L. M., & Homza, B. V. (2014). Fructose compared with glucose is more a potent glycoxidation agent in vitro, but not under carbohydrate-induced stress in vivo: potential role of antioxidant and antiglycation enzymes. Carbohydrate Research, 384, 61-9. https://doi.org/10.1016/j.carres.2013.11.015
Semchyshyn HM, et al. Fructose Compared With Glucose Is More a Potent Glycoxidation Agent in Vitro, but Not Under Carbohydrate-induced Stress in Vivo: Potential Role of Antioxidant and Antiglycation Enzymes. Carbohydr Res. 2014 Jan 30;384:61-9. PubMed PMID: 24361593.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fructose compared with glucose is more a potent glycoxidation agent in vitro, but not under carbohydrate-induced stress in vivo: potential role of antioxidant and antiglycation enzymes. AU - Semchyshyn,Halyna M, AU - Miedzobrodzki,Jacek, AU - Bayliak,Maria M, AU - Lozinska,Liudmyla M, AU - Homza,Bohdana V, Y1 - 2013/12/01/ PY - 2013/09/10/received PY - 2013/11/20/revised PY - 2013/11/21/accepted PY - 2013/12/24/entrez PY - 2013/12/24/pubmed PY - 2014/12/23/medline KW - 2′,7′-dichlorofluorescein diacetate KW - AGEs KW - CFU KW - CP KW - DCHF-DA KW - Defensive enzymes KW - Fructose KW - G6PDH KW - GLO1 KW - GLO2 KW - GR KW - Glucose KW - Glycoxidation KW - In vitro KW - RCS KW - ROS KW - SOD KW - Saccharomyces cerevisiae KW - YPS KW - advanced glycoxidation end-products KW - carbonyl groups in proteins KW - colony forming units KW - glucose-6-phosphate dehydrogenase KW - glutathione reductase KW - glyoxalase I KW - glyoxalase II KW - medium containing yeast extract, peptone and sucrose KW - reactive carbonyl species KW - reactive oxygen species KW - superoxide dismutase SP - 61 EP - 9 JF - Carbohydrate research JO - Carbohydr Res VL - 384 N2 - The contribution of carbohydrates to non-enzymatic processes such as glycation/autoxidation has been extensively investigated over the last decades. This may be attributed to either beneficial or detrimental effects of reducing carbohydrates, and most studies in the field of glycoxidation are focused on glucose. Non-enzymatic reactions of fructose have not been as thoroughly investigated as those of glucose. To compare glucose and fructose involvement in the generation of glycoxidation products under experimental conditions close to the physiological situation, we used intact Saccharomyces cerevisiae cells as in vivo model and cell-free extracts prepared from whole yeast cells as in vitro model. Both intact cells and cell-free extracts were incubated with glucose or fructose. It was shown that: (i) in vitro fructose was more reactive than glucose and produced higher level of autoxidation and glycation products; (ii) no substantive differences were observed for the effect of glucose and fructose on the intracellular level of glycoxidation products, when intact yeast cells were exposed to the high concentration of hexoses; (iii) the activity of defensive enzymes (superoxide dismutase, catalase, glyoxalases, and glutathione reductase) was increased in both glucose- and fructose-stressed yeasts, indicating the development of oxidative/carbonyl stress; (iv) glucose-6-phosphate dehydrogenase activity significantly dropped in yeast exposed to both hexoses, demonstrating its high sensitivity to reactive oxygen and carbonyl species; and (v) fructose more markedly activated glyoxalases than glucose. Involvement of glucose and fructose in the glycoxidation reactions as well as potential role of antioxidant and antiglycation enzymes in yeast protection against glycoxidation are discussed. SN - 1873-426X UR - https://www.unboundmedicine.com/medline/citation/24361593/Fructose_compared_with_glucose_is_more_a_potent_glycoxidation_agent_in_vitro_but_not_under_carbohydrate_induced_stress_in_vivo:_potential_role_of_antioxidant_and_antiglycation_enzymes_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0008-6215(13)00410-2 DB - PRIME DP - Unbound Medicine ER -