Tags

Type your tag names separated by a space and hit enter

Biochemical and histopathological responses of Wistar rats to oral intake of microcystins and cyanobacterial biomass.
Neuro Endocrinol Lett. 2013; 34 Suppl 2:11-20.NE

Abstract

OBJECTIVES

Cyanobacteria are producers of potent and environmentally abundant microcystins, representing an emerging global health issue. In the present study, we investigated the impact of pure microcystins and cyanobacterial biomass on laboratory rats (Wistar albino rats, males, 30 days old) under different exposure scenarios.

METHODS

The rats were fed diets containing fish meat with microcystins in various concentrations and forms (cyanobacterial biomass and isolated microcystins) for 28 days.

RESULTS

Although considerable amounts of microcystins (MCs) were administered to the rats, all levels of MCs in the liver were close to the detection limit (3-5 ng/g fresh weight) using liquid chromatography - tandem mass spectrometry. Only rats exposed to cyanobacterial biomass had clearly higher hepatic and splenic somatic indexes while markers of oxidative stress (glutathione-S-transferase, glutathione reductase, lipid peroxidatio) were significantly increased in the group exposed to the high dose of MCs. Most of the analysed biochemical parameters did not show clear differences among groups. Levels of bilirubin and lipases were significantly increased only after exposure to cyanobacterial biomass and MCs, respectively. Considering microscopic findings in the liver, kidney, thymus, spleen and brain, histopathology was dominated by alterations in the hepatic parenchyma and renal cortical tubular system.

CONCLUSIONS

The present study demonstrates that oral exposure to MCs and cyanobacterial biomass may induce biochemical and detoxification responses associated with damage to liver and kidneys and in the laboratory rat.

Authors+Show Affiliations

Research Centre for Toxic Compounds in the Environment (RECETOX), Masaryk University - Faculty of Science Brno, Czech Republic. adamovsky@recetox.muni.cz.Department of Veterinary Ecology and Environmental Protection, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic.Veterinary Research Institute, Brno, Czech Republic.Department of Ecophysiology and Aquaculture, Leibniz-Institute of Freshwater Ecology and Inland Fisheries, Berlin, Germany.Department of Fishery and Hydrobiology, Faculty of Agronomy, Mendel University, Brno, Czech Republic.Department of Fishery and Hydrobiology, Faculty of Agronomy, Mendel University, Brno, Czech Republic.Department of Veterinary Ecology and Environmental Protection, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic.Research Centre for Toxic Compounds in the Environment (RECETOX), Masaryk University - Faculty of Science Brno, Czech Republic.Research Centre for Toxic Compounds in the Environment (RECETOX), Masaryk University - Faculty of Science Brno, Czech Republic.Research Centre for Toxic Compounds in the Environment (RECETOX), Masaryk University - Faculty of Science Brno, Czech Republic.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24362087

Citation

Adamovsky, Ondrej, et al. "Biochemical and Histopathological Responses of Wistar Rats to Oral Intake of Microcystins and Cyanobacterial Biomass." Neuro Endocrinology Letters, vol. 34 Suppl 2, 2013, pp. 11-20.
Adamovsky O, Palikova M, Ondrackova P, et al. Biochemical and histopathological responses of Wistar rats to oral intake of microcystins and cyanobacterial biomass. Neuro Endocrinol Lett. 2013;34 Suppl 2:11-20.
Adamovsky, O., Palikova, M., Ondrackova, P., Zikova, A., Kopp, R., Mares, J., Pikula, J., Paskerova, H., Kohoutek, J., & Blaha, L. (2013). Biochemical and histopathological responses of Wistar rats to oral intake of microcystins and cyanobacterial biomass. Neuro Endocrinology Letters, 34 Suppl 2, 11-20.
Adamovsky O, et al. Biochemical and Histopathological Responses of Wistar Rats to Oral Intake of Microcystins and Cyanobacterial Biomass. Neuro Endocrinol Lett. 2013;34 Suppl 2:11-20. PubMed PMID: 24362087.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Biochemical and histopathological responses of Wistar rats to oral intake of microcystins and cyanobacterial biomass. AU - Adamovsky,Ondrej, AU - Palikova,Miroslava, AU - Ondrackova,Petra, AU - Zikova,Andrea, AU - Kopp,Radovan, AU - Mares,Jan, AU - Pikula,Jiri, AU - Paskerova,Hana, AU - Kohoutek,Jiri, AU - Blaha,Ludek, PY - 2013/06/21/received PY - 2013/08/30/accepted PY - 2013/12/24/entrez PY - 2013/12/24/pubmed PY - 2014/5/6/medline SP - 11 EP - 20 JF - Neuro endocrinology letters JO - Neuro Endocrinol. Lett. VL - 34 Suppl 2 N2 - OBJECTIVES: Cyanobacteria are producers of potent and environmentally abundant microcystins, representing an emerging global health issue. In the present study, we investigated the impact of pure microcystins and cyanobacterial biomass on laboratory rats (Wistar albino rats, males, 30 days old) under different exposure scenarios. METHODS: The rats were fed diets containing fish meat with microcystins in various concentrations and forms (cyanobacterial biomass and isolated microcystins) for 28 days. RESULTS: Although considerable amounts of microcystins (MCs) were administered to the rats, all levels of MCs in the liver were close to the detection limit (3-5 ng/g fresh weight) using liquid chromatography - tandem mass spectrometry. Only rats exposed to cyanobacterial biomass had clearly higher hepatic and splenic somatic indexes while markers of oxidative stress (glutathione-S-transferase, glutathione reductase, lipid peroxidatio) were significantly increased in the group exposed to the high dose of MCs. Most of the analysed biochemical parameters did not show clear differences among groups. Levels of bilirubin and lipases were significantly increased only after exposure to cyanobacterial biomass and MCs, respectively. Considering microscopic findings in the liver, kidney, thymus, spleen and brain, histopathology was dominated by alterations in the hepatic parenchyma and renal cortical tubular system. CONCLUSIONS: The present study demonstrates that oral exposure to MCs and cyanobacterial biomass may induce biochemical and detoxification responses associated with damage to liver and kidneys and in the laboratory rat. SN - 0172-780X UR - https://www.unboundmedicine.com/medline/citation/24362087/Biochemical_and_histopathological_responses_of_Wistar_rats_to_oral_intake_of_microcystins_and_cyanobacterial_biomass_ DB - PRIME DP - Unbound Medicine ER -