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Rapid and sensitive liquid chromatography coupled with electrospray ionization tandem mass spectrometry method for the analysis of paclitaxel, docetaxel, vinblastine, and vinorelbine in human plasma.
Ther Drug Monit. 2014 Jun; 36(3):394-400.TD

Abstract

BACKGROUND

A rapid and sensitive analytical method using liquid chromatography coupled with electrospray ionization tandem mass spectrometry (LC-MS/MS) was developed for the determination of paclitaxel, docetaxel, vinblastine, and vinorelbine in human plasma.

METHODS

A simple liquid-liquid extraction procedure was applied using only 100-μL plasma. Chromatographic separation of these anticancer drugs was achieved with an isocratic mobile phase consisting of acetonitrile/aqueous buffer (10 mmol/L ammonium acetate and 0.1% formic acid in 70:30, vol/vol) at a flow rate of 0.25 mL/min in a short time (4.5 minutes).

RESULTS

The calibration curves for paclitaxel, docetaxel, vinblastine, and vinorelbine in spiked human plasma ranged from 25 to 2500, 10 to 1000, 10 to 1000, and 10 to 1000 ng/mL, respectively. The squares of the linear correlation coefficients were all more than 0.99. The intraday and interday relative standard deviations across 3 validation runs over the entire concentration range were less than 9.2%.

CONCLUSIONS

The established method should be helpful for the pharmacokinetic monitoring of paclitaxel, docetaxel, vinblastine, and vinorelbine in the human plasma of non-small cell lung cancer patients.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Gao S
Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai, China.
Zhou J
No affiliation info available
Zhang F
No affiliation info available
Miao H
No affiliation info available
Yun Y
No affiliation info available
Feng J
No affiliation info available
Tao X
No affiliation info available
Chen W
No affiliation info available

MeSH

Antineoplastic AgentsCalibrationCarcinoma, Non-Small-Cell LungChromatography, High Pressure LiquidDocetaxelDose-Response Relationship, DrugDrug MonitoringFemaleHumansLung NeoplasmsMalePaclitaxelSensitivity and SpecificitySpectrometry, Mass, Electrospray IonizationTandem Mass SpectrometryTaxoidsVinblastineVinorelbine

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24365981

Citation

Gao, Shouhong, et al. "Rapid and Sensitive Liquid Chromatography Coupled With Electrospray Ionization Tandem Mass Spectrometry Method for the Analysis of Paclitaxel, Docetaxel, Vinblastine, and Vinorelbine in Human Plasma." Therapeutic Drug Monitoring, vol. 36, no. 3, 2014, pp. 394-400.
Gao S, Zhou J, Zhang F, et al. Rapid and sensitive liquid chromatography coupled with electrospray ionization tandem mass spectrometry method for the analysis of paclitaxel, docetaxel, vinblastine, and vinorelbine in human plasma. Ther Drug Monit. 2014;36(3):394-400.
Gao, S., Zhou, J., Zhang, F., Miao, H., Yun, Y., Feng, J., Tao, X., & Chen, W. (2014). Rapid and sensitive liquid chromatography coupled with electrospray ionization tandem mass spectrometry method for the analysis of paclitaxel, docetaxel, vinblastine, and vinorelbine in human plasma. Therapeutic Drug Monitoring, 36(3), 394-400. https://doi.org/10.1097/FTD.0000000000000010
Gao S, et al. Rapid and Sensitive Liquid Chromatography Coupled With Electrospray Ionization Tandem Mass Spectrometry Method for the Analysis of Paclitaxel, Docetaxel, Vinblastine, and Vinorelbine in Human Plasma. Ther Drug Monit. 2014;36(3):394-400. PubMed PMID: 24365981.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid and sensitive liquid chromatography coupled with electrospray ionization tandem mass spectrometry method for the analysis of paclitaxel, docetaxel, vinblastine, and vinorelbine in human plasma. AU - Gao,Shouhong, AU - Zhou,Jingya, AU - Zhang,Feng, AU - Miao,Haijun, AU - Yun,Yunlei, AU - Feng,Jin, AU - Tao,Xia, AU - Chen,Wansheng, PY - 2013/12/25/entrez PY - 2013/12/25/pubmed PY - 2015/1/13/medline SP - 394 EP - 400 JF - Therapeutic drug monitoring JO - Ther Drug Monit VL - 36 IS - 3 N2 - BACKGROUND: A rapid and sensitive analytical method using liquid chromatography coupled with electrospray ionization tandem mass spectrometry (LC-MS/MS) was developed for the determination of paclitaxel, docetaxel, vinblastine, and vinorelbine in human plasma. METHODS: A simple liquid-liquid extraction procedure was applied using only 100-μL plasma. Chromatographic separation of these anticancer drugs was achieved with an isocratic mobile phase consisting of acetonitrile/aqueous buffer (10 mmol/L ammonium acetate and 0.1% formic acid in 70:30, vol/vol) at a flow rate of 0.25 mL/min in a short time (4.5 minutes). RESULTS: The calibration curves for paclitaxel, docetaxel, vinblastine, and vinorelbine in spiked human plasma ranged from 25 to 2500, 10 to 1000, 10 to 1000, and 10 to 1000 ng/mL, respectively. The squares of the linear correlation coefficients were all more than 0.99. The intraday and interday relative standard deviations across 3 validation runs over the entire concentration range were less than 9.2%. CONCLUSIONS: The established method should be helpful for the pharmacokinetic monitoring of paclitaxel, docetaxel, vinblastine, and vinorelbine in the human plasma of non-small cell lung cancer patients. SN - 1536-3694 UR - https://www.unboundmedicine.com/medline/citation/24365981/Rapid_and_sensitive_liquid_chromatography_coupled_with_electrospray_ionization_tandem_mass_spectrometry_method_for_the_analysis_of_paclitaxel_docetaxel_vinblastine_and_vinorelbine_in_human_plasma_ DB - PRIME DP - Unbound Medicine ER -
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