Tags

Type your tag names separated by a space and hit enter

Inhibitory effects of 4-hydroxyderricin and xanthoangelol on lipopolysaccharide-induced inflammatory responses in RAW264 macrophages.
J Agric Food Chem. 2014 Jan 15; 62(2):462-7.JA

Abstract

The Japanese herb, Ashitaba (Angelica keiskei Koidzumi), contains two prenylated chalcones, 4-hydroxyderricin and xanthoangelol, which are considered to be the major active compounds of Ashitaba. However, their effects on inflammatory responses are poorly understood. In the present study, we investigated the effects and underlying molecular mechanisms of 4-hydroxyderricin and xanthoangelol on lipopolysaccharide (LPS)-induced inflammatory responses in RAW264 mouse macrophages. LPS-mediated production of nitric oxide (NO) was markedly reduced by 4-hydroxyderricin (10 μM) and xanthoangelol (5 μM) compared with their parent compound, chalcone (25 μM). They also inhibited LPS-induced secretion of tumor necrosis factor-alpha (TNF-α) and expression of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2). Although chalcone decreased the DNA-binding activity of both activator protein-1 (AP-1) and nuclear factor-kappa B (NF-κB), 4-hydroxyderricin and xanthoangelol suppressed only AP-1 and had no effect on NF-κB. On the other hand, all of the tested chalcones reduced the phosphorylation (at serine 536) level of the p65 subunit of NF-κB. 4-Hydroxyderricin and xanthoangelol may be promising for the prevention of inflammatory diseases.

Authors+Show Affiliations

Organization of Advanced Science and Technology, Kobe University , 1-1 Rokkodai-cho, Nada-ku, Kobe 657-8501, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24369884

Citation

Yasuda, Michiko, et al. "Inhibitory Effects of 4-hydroxyderricin and Xanthoangelol On Lipopolysaccharide-induced Inflammatory Responses in RAW264 Macrophages." Journal of Agricultural and Food Chemistry, vol. 62, no. 2, 2014, pp. 462-7.
Yasuda M, Kawabata K, Miyashita M, et al. Inhibitory effects of 4-hydroxyderricin and xanthoangelol on lipopolysaccharide-induced inflammatory responses in RAW264 macrophages. J Agric Food Chem. 2014;62(2):462-7.
Yasuda, M., Kawabata, K., Miyashita, M., Okumura, M., Yamamoto, N., Takahashi, M., Ashida, H., & Ohigashi, H. (2014). Inhibitory effects of 4-hydroxyderricin and xanthoangelol on lipopolysaccharide-induced inflammatory responses in RAW264 macrophages. Journal of Agricultural and Food Chemistry, 62(2), 462-7. https://doi.org/10.1021/jf404175t
Yasuda M, et al. Inhibitory Effects of 4-hydroxyderricin and Xanthoangelol On Lipopolysaccharide-induced Inflammatory Responses in RAW264 Macrophages. J Agric Food Chem. 2014 Jan 15;62(2):462-7. PubMed PMID: 24369884.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibitory effects of 4-hydroxyderricin and xanthoangelol on lipopolysaccharide-induced inflammatory responses in RAW264 macrophages. AU - Yasuda,Michiko, AU - Kawabata,Kyuichi, AU - Miyashita,Miki, AU - Okumura,Mayu, AU - Yamamoto,Norio, AU - Takahashi,Masakazu, AU - Ashida,Hitoshi, AU - Ohigashi,Hajime, Y1 - 2014/01/06/ PY - 2013/12/28/entrez PY - 2013/12/29/pubmed PY - 2015/9/5/medline KW - 4-hydroxyderrin KW - AP-1 KW - Angelica keiskei Koidzumi (Ashitaba) KW - NF-κB KW - anti-inflammation KW - xanthoangelol SP - 462 EP - 7 JF - Journal of agricultural and food chemistry JO - J. Agric. Food Chem. VL - 62 IS - 2 N2 - The Japanese herb, Ashitaba (Angelica keiskei Koidzumi), contains two prenylated chalcones, 4-hydroxyderricin and xanthoangelol, which are considered to be the major active compounds of Ashitaba. However, their effects on inflammatory responses are poorly understood. In the present study, we investigated the effects and underlying molecular mechanisms of 4-hydroxyderricin and xanthoangelol on lipopolysaccharide (LPS)-induced inflammatory responses in RAW264 mouse macrophages. LPS-mediated production of nitric oxide (NO) was markedly reduced by 4-hydroxyderricin (10 μM) and xanthoangelol (5 μM) compared with their parent compound, chalcone (25 μM). They also inhibited LPS-induced secretion of tumor necrosis factor-alpha (TNF-α) and expression of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2). Although chalcone decreased the DNA-binding activity of both activator protein-1 (AP-1) and nuclear factor-kappa B (NF-κB), 4-hydroxyderricin and xanthoangelol suppressed only AP-1 and had no effect on NF-κB. On the other hand, all of the tested chalcones reduced the phosphorylation (at serine 536) level of the p65 subunit of NF-κB. 4-Hydroxyderricin and xanthoangelol may be promising for the prevention of inflammatory diseases. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/24369884/Inhibitory_effects_of_4_hydroxyderricin_and_xanthoangelol_on_lipopolysaccharide_induced_inflammatory_responses_in_RAW264_macrophages_ L2 - https://dx.doi.org/10.1021/jf404175t DB - PRIME DP - Unbound Medicine ER -