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"Polymeromics": Mass spectrometry based strategies in polymer science toward complete sequencing approaches: a review.
Anal Chim Acta. 2014 Jan 15; 808:56-69.AC

Abstract

Mass spectrometry (MS) is the most versatile and comprehensive method in "OMICS" sciences (i.e. in proteomics, genomics, metabolomics and lipidomics). The applications of MS and tandem MS (MS/MS or MS(n)) provide sequence information of the full complement of biological samples in order to understand the importance of the sequences on their precise and specific functions. Nowadays, the control of polymer sequences and their accurate characterization is one of the significant challenges of current polymer science. Therefore, a similar approach can be very beneficial for characterizing and understanding the complex structures of synthetic macromolecules. MS-based strategies allow a relatively precise examination of polymeric structures (e.g. their molar mass distributions, monomer units, side chain substituents, end-group functionalities, and copolymer compositions). Moreover, tandem MS offer accurate structural information from intricate macromolecular structures; however, it produces vast amount of data to interpret. In "OMICS" sciences, the software application to interpret the obtained data has developed satisfyingly (e.g. in proteomics), because it is not possible to handle the amount of data acquired via (tandem) MS studies on the biological samples manually. It can be expected that special software tools will improve the interpretation of (tandem) MS output from the investigations of synthetic polymers as well. Eventually, the MS/MS field will also open up for polymer scientists who are not MS-specialists. In this review, we dissect the overall framework of the MS and MS/MS analysis of synthetic polymers into its key components. We discuss the fundamentals of polymer analyses as well as recent advances in the areas of tandem mass spectrometry, software developments, and the overall future perspectives on the way to polymer sequencing, one of the last Holy Grail in polymer science.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Altuntaş E
Laboratory of Organic and Macromolecular Chemistry (IOMC), Friedrich Schiller University Jena, Humboldtstr. 10, 07743 Jena, Germany; Jena Center for Soft Matter (JCSM), Friedrich Schiller University Jena, Philosophenweg 7, 07743 Jena, Germany.
Schubert US
Laboratory of Organic and Macromolecular Chemistry (IOMC), Friedrich Schiller University Jena, Humboldtstr. 10, 07743 Jena, Germany; Jena Center for Soft Matter (JCSM), Friedrich Schiller University Jena, Philosophenweg 7, 07743 Jena, Germany; Dutch Polymer Institute (DPI), P.O. Box 902, 5600 AX Eindhoven, The Netherlands. Electronic address: ulrich.schubert@uni-jena.de.

MeSH

Chromatography, High Pressure LiquidMass SpectrometryPolymersSoftwareSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationTandem Mass Spectrometry

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Review

Language

eng

PubMed ID

24370093

Citation

Altuntaş, Esra, and Ulrich S. Schubert. ""Polymeromics": Mass Spectrometry Based Strategies in Polymer Science Toward Complete Sequencing Approaches: a Review." Analytica Chimica Acta, vol. 808, 2014, pp. 56-69.
Altuntaş E, Schubert US. "Polymeromics": Mass spectrometry based strategies in polymer science toward complete sequencing approaches: a review. Anal Chim Acta. 2014;808:56-69.
Altuntaş, E., & Schubert, U. S. (2014). "Polymeromics": Mass spectrometry based strategies in polymer science toward complete sequencing approaches: a review. Analytica Chimica Acta, 808, 56-69. https://doi.org/10.1016/j.aca.2013.10.027
Altuntaş E, Schubert US. "Polymeromics": Mass Spectrometry Based Strategies in Polymer Science Toward Complete Sequencing Approaches: a Review. Anal Chim Acta. 2014 Jan 15;808:56-69. PubMed PMID: 24370093.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - "Polymeromics": Mass spectrometry based strategies in polymer science toward complete sequencing approaches: a review. AU - Altuntaş,Esra, AU - Schubert,Ulrich S, Y1 - 2013/10/21/ PY - 2013/07/08/received PY - 2013/10/07/revised PY - 2013/10/11/accepted PY - 2013/12/28/entrez PY - 2013/12/29/pubmed PY - 2014/8/13/medline KW - 2D-LC KW - APCI KW - AUC KW - CCE KW - CID KW - CRP KW - ECD KW - ESI KW - FTICR KW - FTMS KW - Fourier transform ion cyclotron resonance KW - Fourier transform mass spectrometry KW - GMA KW - GPEC KW - HPLC KW - IMS or IMMS KW - LAC KW - LC KW - LCCC KW - LEAC KW - LS KW - MALDI KW - MMA KW - MMD KW - MS KW - MS/MS KW - Mass spectrometry KW - NMR KW - ODNs KW - PCL KW - PDI KW - PDMS KW - PE KW - PEG KW - PEI KW - PEO KW - PEPOx KW - PET KW - PEtOx KW - PLUMS KW - PMA KW - PMAA KW - PMMA KW - POx KW - PP KW - PPO KW - PS KW - Polymer science KW - Polymer sequencing KW - Polymeromics KW - SEC KW - UV–vis KW - analytical ultracentrifugation KW - atmospheric pressure chemical ionization KW - characteristic collision energy KW - collision-induced dissociation KW - controlled radical polymerization KW - electron capture dissociation KW - electrospray ionization KW - glycidyl methacrylate KW - gradient polymer elution chromatography KW - high-performance liquid chromatography KW - ion mobility (mass) spectrometry KW - light scattering KW - liquid adsorption chromatography KW - liquid chromatography KW - liquid chromatography at critical conditions KW - liquid exclusion adsorption chromatography KW - mass spectrometry KW - matrix-assisted laser desorption/ionization KW - methyl methacrylate KW - molar-mass distribution KW - nuclear magnetic resonance spectroscopy KW - oligodeoxynucleotides KW - poly(2-(1-ethylpentyl)-2-oxazoline) KW - poly(2-ethyl-2-oxazoline) KW - poly(2-oxazoline)s KW - poly(dimethylsiloxane) KW - poly(ethylene glycol) KW - poly(ethylene imine) KW - poly(ethylene oxide) KW - poly(ethylene terephthalate) KW - poly(ethylene) KW - poly(methacrylate) KW - poly(methacrylic acid) KW - poly(methyl methacrylate) KW - poly(propylene oxide) KW - poly(propylene) KW - poly(styrene) KW - poly(ɛ-caprolactone) KW - polydispersity index KW - polymer labeling using mass spectrometry KW - size-exclusion chromatography KW - tandem mass spectrometry KW - two-dimensional liquid chromatography KW - ultraviolet–visible spectroscopy SP - 56 EP - 69 JF - Analytica chimica acta JO - Anal Chim Acta VL - 808 N2 - Mass spectrometry (MS) is the most versatile and comprehensive method in "OMICS" sciences (i.e. in proteomics, genomics, metabolomics and lipidomics). The applications of MS and tandem MS (MS/MS or MS(n)) provide sequence information of the full complement of biological samples in order to understand the importance of the sequences on their precise and specific functions. Nowadays, the control of polymer sequences and their accurate characterization is one of the significant challenges of current polymer science. Therefore, a similar approach can be very beneficial for characterizing and understanding the complex structures of synthetic macromolecules. MS-based strategies allow a relatively precise examination of polymeric structures (e.g. their molar mass distributions, monomer units, side chain substituents, end-group functionalities, and copolymer compositions). Moreover, tandem MS offer accurate structural information from intricate macromolecular structures; however, it produces vast amount of data to interpret. In "OMICS" sciences, the software application to interpret the obtained data has developed satisfyingly (e.g. in proteomics), because it is not possible to handle the amount of data acquired via (tandem) MS studies on the biological samples manually. It can be expected that special software tools will improve the interpretation of (tandem) MS output from the investigations of synthetic polymers as well. Eventually, the MS/MS field will also open up for polymer scientists who are not MS-specialists. In this review, we dissect the overall framework of the MS and MS/MS analysis of synthetic polymers into its key components. We discuss the fundamentals of polymer analyses as well as recent advances in the areas of tandem mass spectrometry, software developments, and the overall future perspectives on the way to polymer sequencing, one of the last Holy Grail in polymer science. SN - 1873-4324 UR - https://www.unboundmedicine.com/medline/citation/24370093/"Polymeromics":_Mass_spectrometry_based_strategies_in_polymer_science_toward_complete_sequencing_approaches:_a_review_ DB - PRIME DP - Unbound Medicine ER -