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Vitamin D status and vitamin D receptor gene polymorphisms and susceptibility to type 1 diabetes in Egyptian children.
Gene. 2014 Feb 25; 536(2):430-4.GENE

Abstract

BACKGROUND

Type 1 diabetes mellitus (T1DM) is recognized as a T-cell-mediated autoimmune disease. Vitamin D compounds are known to suppress T-cell activation by binding to vitamin D receptor (VDR); and thus, VDR gene polymorphisms may be related to T-cell-mediated autoimmune diseases. The aim of this study was to investigate the association between vitamin D status and VDR gene polymorphisms and T1DM.

MATERIALS AND METHODS

One hundred and twenty patients with T1DM and one hundred and twenty controls were enrolled in the study. VDR gene BsmI, FokI, ApaI and TaqI polymorphisms were determined using polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). Serum 25-hydroxyvitamin D (25(OH)D) was determined using ELISA.

RESULT

Serum 25(OH)D levels revealed a vitamin D deficiency or insufficiency in 75% of the patients. The mean levels of vitamin D were significantly lower in patients as compared to their controls (P=<0.001). VDR BsmI Bb and bb genotypes and VDR FokI Ff and ff genotypes were associated with increased risk of T1DM (OR=2.3, 95% CI=1.3-4.2, P=0.005; OR=2.2, 95% CI=1.1-4.7, P=0.04; OR=1.8, 95% CI=1.03-3.04, P=0.04; OR=4.03, 95% CI=1.2-13.1, P=0.01 respectively), while the VDR ApaI and TaqI polymorphisms were not.

CONCLUSION

Our study indicated that vitamin D deficiency and VDR BsmI and FokI polymorphisms were associated with T1DM in Egyptian children.

Authors+Show Affiliations

Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt. Electronic address: hebapasha@yahoo.com.Clinical Pathology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.Pediatric Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24370753

Citation

Abd-Allah, Somia H., et al. "Vitamin D Status and Vitamin D Receptor Gene Polymorphisms and Susceptibility to Type 1 Diabetes in Egyptian Children." Gene, vol. 536, no. 2, 2014, pp. 430-4.
Abd-Allah SH, Pasha HF, Hagrass HA, et al. Vitamin D status and vitamin D receptor gene polymorphisms and susceptibility to type 1 diabetes in Egyptian children. Gene. 2014;536(2):430-4.
Abd-Allah, S. H., Pasha, H. F., Hagrass, H. A., & Alghobashy, A. A. (2014). Vitamin D status and vitamin D receptor gene polymorphisms and susceptibility to type 1 diabetes in Egyptian children. Gene, 536(2), 430-4. https://doi.org/10.1016/j.gene.2013.12.032
Abd-Allah SH, et al. Vitamin D Status and Vitamin D Receptor Gene Polymorphisms and Susceptibility to Type 1 Diabetes in Egyptian Children. Gene. 2014 Feb 25;536(2):430-4. PubMed PMID: 24370753.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Vitamin D status and vitamin D receptor gene polymorphisms and susceptibility to type 1 diabetes in Egyptian children. AU - Abd-Allah,Somia H, AU - Pasha,Heba F, AU - Hagrass,Hoda A, AU - Alghobashy,Ashgan A, Y1 - 2013/12/23/ PY - 2013/08/04/received PY - 2013/11/20/revised PY - 2013/12/13/accepted PY - 2013/12/28/entrez PY - 2013/12/29/pubmed PY - 2014/4/29/medline KW - 25(OH)D KW - 25-hydroxyvitamin D KW - BMI KW - Gene polymorphism KW - PCR-RFLP KW - SNPs KW - T1DM KW - Type 1 diabetes mellitus KW - VDR KW - Vitamin D KW - Vitamin D receptor KW - body mass index KW - polymerase chain reaction restriction fragment length polymorphism KW - single nucleotide polymorphisms KW - type 1 diabetes mellitus KW - vitamin D receptor SP - 430 EP - 4 JF - Gene JO - Gene VL - 536 IS - 2 N2 - BACKGROUND: Type 1 diabetes mellitus (T1DM) is recognized as a T-cell-mediated autoimmune disease. Vitamin D compounds are known to suppress T-cell activation by binding to vitamin D receptor (VDR); and thus, VDR gene polymorphisms may be related to T-cell-mediated autoimmune diseases. The aim of this study was to investigate the association between vitamin D status and VDR gene polymorphisms and T1DM. MATERIALS AND METHODS: One hundred and twenty patients with T1DM and one hundred and twenty controls were enrolled in the study. VDR gene BsmI, FokI, ApaI and TaqI polymorphisms were determined using polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). Serum 25-hydroxyvitamin D (25(OH)D) was determined using ELISA. RESULT: Serum 25(OH)D levels revealed a vitamin D deficiency or insufficiency in 75% of the patients. The mean levels of vitamin D were significantly lower in patients as compared to their controls (P=<0.001). VDR BsmI Bb and bb genotypes and VDR FokI Ff and ff genotypes were associated with increased risk of T1DM (OR=2.3, 95% CI=1.3-4.2, P=0.005; OR=2.2, 95% CI=1.1-4.7, P=0.04; OR=1.8, 95% CI=1.03-3.04, P=0.04; OR=4.03, 95% CI=1.2-13.1, P=0.01 respectively), while the VDR ApaI and TaqI polymorphisms were not. CONCLUSION: Our study indicated that vitamin D deficiency and VDR BsmI and FokI polymorphisms were associated with T1DM in Egyptian children. SN - 1879-0038 UR - https://www.unboundmedicine.com/medline/citation/24370753/Vitamin_D_status_and_vitamin_D_receptor_gene_polymorphisms_and_susceptibility_to_type_1_diabetes_in_Egyptian_children_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-1119(13)01693-4 DB - PRIME DP - Unbound Medicine ER -