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Molecular characterization of a novel hepatitis E virus (HEV) strain obtained from a wild boar in Japan that is highly divergent from the previously recognized HEV strains.
Virus Res. 2014 Feb 13; 180:59-69.VR

Abstract

Although a consensus classification system for hepatitis E virus (HEV) genotypes is currently unavailable, HEV variants (JBOAR135-Shiz09 and wbJOY_06) from wild boars (Sus scrofa leucomystax) have provisionally been classified into two novel genotypes (5 and 6). While performing a survey of HEV infections among 566 wild boars that were captured in Japan between January 2010 and August 2013, we found 24 boars (4.2%) with ongoing HEV infections: 13 had genotype 3 HEV, 10 had genotype 4 HEV and the remaining boar possessed a novel HEV variant (designated wbJNN_13). The entire wbJNN_13 genome comprised 7247 nucleotides excluding the poly(A) tail, and was highly divergent from known genotype 1 to 4 HEV isolates derived from humans, swine, wild boars, deer, mongoose and rabbits by 22.4-28.2%, JBOAR135-Shiz09 and wbJOY_06 by 19.6-21.9% and rat, ferret, bat and avian HEV isolates by 40.9-46.1% over the entire genome. Phylogenetic trees confirmed that wbJNN_13 is distantly related to all known HEV isolates. A Simplot analysis revealed no significant recombination among the existing HEV strains. These results indicate the presence of at least three genetic lineages of presumably boar-indigenous HEV strains. Further studies to fully understand the extent of the genomic heterogeneity of HEV variants infecting wild boars are warranted.

Authors+Show Affiliations

Division of Virology, Department of Infection and Immunity, Jichi Medical University School of Medicine, 3311-1 Yakushiji, Shimotsuke-shi, Tochigi 329-0498, Japan.Division of Virology, Department of Infection and Immunity, Jichi Medical University School of Medicine, 3311-1 Yakushiji, Shimotsuke-shi, Tochigi 329-0498, Japan.Division of Virology, Department of Infection and Immunity, Jichi Medical University School of Medicine, 3311-1 Yakushiji, Shimotsuke-shi, Tochigi 329-0498, Japan.Division of Virology, Department of Infection and Immunity, Jichi Medical University School of Medicine, 3311-1 Yakushiji, Shimotsuke-shi, Tochigi 329-0498, Japan.Hepatology Research Center, Okayama Saiseikai General Hospital, Okayama-shi, Okayama 700-8511, Japan.Nara Municipal Tahara Clinic, Nara-shi, Nara 630-2174, Japan.Tessei-cho Clinic, Niimi-shi, Okayama 719-3701, Japan.Miyazaki Prefectural Institute for Public Health and Environment, Miyazaki-shi, Miyazaki 889-2155, Japan.Osaba Family Clinic, Yamaguchi-shi, Yamaguchi 753-0212, Japan.Division of Internal Medicine, Okayama University Hospital Misasa Medical Center, Misasa, Tottori 682-0122, Japan.Kamiichi General Hospital, Kamiichi, Toyama 930-0391, Japan.Division of Virology, Department of Infection and Immunity, Jichi Medical University School of Medicine, 3311-1 Yakushiji, Shimotsuke-shi, Tochigi 329-0498, Japan. Electronic address: hokamoto@jichi.ac.jp.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24370869

Citation

Takahashi, Masaharu, et al. "Molecular Characterization of a Novel Hepatitis E Virus (HEV) Strain Obtained From a Wild Boar in Japan That Is Highly Divergent From the Previously Recognized HEV Strains." Virus Research, vol. 180, 2014, pp. 59-69.
Takahashi M, Nishizawa T, Nagashima S, et al. Molecular characterization of a novel hepatitis E virus (HEV) strain obtained from a wild boar in Japan that is highly divergent from the previously recognized HEV strains. Virus Res. 2014;180:59-69.
Takahashi, M., Nishizawa, T., Nagashima, S., Jirintai, S., Kawakami, M., Sonoda, Y., Suzuki, T., Yamamoto, S., Shigemoto, K., Ashida, K., Sato, Y., & Okamoto, H. (2014). Molecular characterization of a novel hepatitis E virus (HEV) strain obtained from a wild boar in Japan that is highly divergent from the previously recognized HEV strains. Virus Research, 180, 59-69. https://doi.org/10.1016/j.virusres.2013.12.014
Takahashi M, et al. Molecular Characterization of a Novel Hepatitis E Virus (HEV) Strain Obtained From a Wild Boar in Japan That Is Highly Divergent From the Previously Recognized HEV Strains. Virus Res. 2014 Feb 13;180:59-69. PubMed PMID: 24370869.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular characterization of a novel hepatitis E virus (HEV) strain obtained from a wild boar in Japan that is highly divergent from the previously recognized HEV strains. AU - Takahashi,Masaharu, AU - Nishizawa,Tsutomu, AU - Nagashima,Shigeo, AU - Jirintai,Suljid, AU - Kawakami,Manri, AU - Sonoda,Yoshihide, AU - Suzuki,Tadahiro, AU - Yamamoto,Shogo, AU - Shigemoto,Kazuhiro, AU - Ashida,Kozo, AU - Sato,Yukihiro, AU - Okamoto,Hiroaki, Y1 - 2013/12/24/ PY - 2013/11/07/received PY - 2013/12/09/revised PY - 2013/12/10/accepted PY - 2013/12/28/entrez PY - 2013/12/29/pubmed PY - 2014/9/18/medline KW - Genotype KW - Hepatitis E virus KW - Phylogeny KW - Wild boar SP - 59 EP - 69 JF - Virus research JO - Virus Res VL - 180 N2 - Although a consensus classification system for hepatitis E virus (HEV) genotypes is currently unavailable, HEV variants (JBOAR135-Shiz09 and wbJOY_06) from wild boars (Sus scrofa leucomystax) have provisionally been classified into two novel genotypes (5 and 6). While performing a survey of HEV infections among 566 wild boars that were captured in Japan between January 2010 and August 2013, we found 24 boars (4.2%) with ongoing HEV infections: 13 had genotype 3 HEV, 10 had genotype 4 HEV and the remaining boar possessed a novel HEV variant (designated wbJNN_13). The entire wbJNN_13 genome comprised 7247 nucleotides excluding the poly(A) tail, and was highly divergent from known genotype 1 to 4 HEV isolates derived from humans, swine, wild boars, deer, mongoose and rabbits by 22.4-28.2%, JBOAR135-Shiz09 and wbJOY_06 by 19.6-21.9% and rat, ferret, bat and avian HEV isolates by 40.9-46.1% over the entire genome. Phylogenetic trees confirmed that wbJNN_13 is distantly related to all known HEV isolates. A Simplot analysis revealed no significant recombination among the existing HEV strains. These results indicate the presence of at least three genetic lineages of presumably boar-indigenous HEV strains. Further studies to fully understand the extent of the genomic heterogeneity of HEV variants infecting wild boars are warranted. SN - 1872-7492 UR - https://www.unboundmedicine.com/medline/citation/24370869/Molecular_characterization_of_a_novel_hepatitis_E_virus__HEV__strain_obtained_from_a_wild_boar_in_Japan_that_is_highly_divergent_from_the_previously_recognized_HEV_strains_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168-1702(13)00443-7 DB - PRIME DP - Unbound Medicine ER -