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Silver nanoparticles stimulate glycogenolysis in rainbow trout (Oncorhynchus mykiss) hepatocytes.
Aquat Toxicol. 2014 Feb; 147:68-75.AT

Abstract

Silver nanoparticles (AgNPs) are found in many consumer products yet their biological effects on non-target aquatic organisms are yet to be fully understood. This research aimed to investigate the effects of AgNPs on cell signaling in rainbow trout (Oncorhynchus mykiss) hepatocytes. We focused on the β-adrenoreceptor (AR), which mediates glycogenolysis, and the glucocorticoid receptor (GCR), which mediates gluconeogenesis. These two receptors have been extensively studied in trout hepatocytes due to their key roles during the stress response to increase glucose availability (among other things), allowing the organisms to cope with the stressor. We show for the first time that AgNPs at a concentration of 1 μg/mL did not interfere with the function of either the β-AR or the GCR systems in rainbow trout hepatocytes, but at the concentration of 10 μg/mL AgNPs stimulated glycogenolysis which was apparently receptor-independent. This study suggests that AgNPs could affect hormone-regulated cell signaling pathways at a concentration of 10 μg/mL.

Authors+Show Affiliations

Department of Biology & Centre for Advanced Research in Environmental Genomics, University of Ottawa, Ottawa, Ontario, Canada. Electronic address: amass017@uottawa.ca.Department of Biology & Centre for Advanced Research in Environmental Genomics, University of Ottawa, Ottawa, Ontario, Canada.Department of Biology & Centre for Advanced Research in Environmental Genomics, University of Ottawa, Ottawa, Ontario, Canada.Department of Biology & Centre for Advanced Research in Environmental Genomics, University of Ottawa, Ottawa, Ontario, Canada.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24374849

Citation

Massarsky, Andrey, et al. "Silver Nanoparticles Stimulate Glycogenolysis in Rainbow Trout (Oncorhynchus Mykiss) Hepatocytes." Aquatic Toxicology (Amsterdam, Netherlands), vol. 147, 2014, pp. 68-75.
Massarsky A, Labarre J, Trudeau VL, et al. Silver nanoparticles stimulate glycogenolysis in rainbow trout (Oncorhynchus mykiss) hepatocytes. Aquat Toxicol. 2014;147:68-75.
Massarsky, A., Labarre, J., Trudeau, V. L., & Moon, T. W. (2014). Silver nanoparticles stimulate glycogenolysis in rainbow trout (Oncorhynchus mykiss) hepatocytes. Aquatic Toxicology (Amsterdam, Netherlands), 147, 68-75. https://doi.org/10.1016/j.aquatox.2013.11.026
Massarsky A, et al. Silver Nanoparticles Stimulate Glycogenolysis in Rainbow Trout (Oncorhynchus Mykiss) Hepatocytes. Aquat Toxicol. 2014;147:68-75. PubMed PMID: 24374849.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Silver nanoparticles stimulate glycogenolysis in rainbow trout (Oncorhynchus mykiss) hepatocytes. AU - Massarsky,Andrey, AU - Labarre,Justine, AU - Trudeau,Vance L, AU - Moon,Thomas W, Y1 - 2013/12/12/ PY - 2013/10/07/received PY - 2013/11/25/revised PY - 2013/11/30/accepted PY - 2013/12/31/entrez PY - 2014/1/1/pubmed PY - 2014/4/18/medline KW - Glucocorticoid receptor KW - Glycogenolysis KW - Nanomaterials KW - Nanosilver KW - Rainbow trout KW - β-Adrenoreceptor SP - 68 EP - 75 JF - Aquatic toxicology (Amsterdam, Netherlands) JO - Aquat Toxicol VL - 147 N2 - Silver nanoparticles (AgNPs) are found in many consumer products yet their biological effects on non-target aquatic organisms are yet to be fully understood. This research aimed to investigate the effects of AgNPs on cell signaling in rainbow trout (Oncorhynchus mykiss) hepatocytes. We focused on the β-adrenoreceptor (AR), which mediates glycogenolysis, and the glucocorticoid receptor (GCR), which mediates gluconeogenesis. These two receptors have been extensively studied in trout hepatocytes due to their key roles during the stress response to increase glucose availability (among other things), allowing the organisms to cope with the stressor. We show for the first time that AgNPs at a concentration of 1 μg/mL did not interfere with the function of either the β-AR or the GCR systems in rainbow trout hepatocytes, but at the concentration of 10 μg/mL AgNPs stimulated glycogenolysis which was apparently receptor-independent. This study suggests that AgNPs could affect hormone-regulated cell signaling pathways at a concentration of 10 μg/mL. SN - 1879-1514 UR - https://www.unboundmedicine.com/medline/citation/24374849/Silver_nanoparticles_stimulate_glycogenolysis_in_rainbow_trout__Oncorhynchus_mykiss__hepatocytes_ DB - PRIME DP - Unbound Medicine ER -