Tags

Type your tag names separated by a space and hit enter

Identification of HilD-regulated genes in Salmonella enterica serovar Typhimurium.
J Bacteriol. 2014 Mar; 196(5):1094-101.JB

Abstract

Salmonella enterica serovar Typhimurium (S. Typhimurium) pathogenicity island 1 (SPI-1) encodes a type III secretion system required for invasion of host gut epithelial cells. Expression of SPI-1 virulence genes is controlled by a complex hierarchy of transcription factors encoded within and outside SPI-1. The master regulator of SPI-1, HilA, is itself regulated by three homologous transcription factors, HilD, HilC, and RtsA. HilD activates transcription of hilA and other target genes in response to environmental conditions associated with the intestinal microenvironment of the host. We have mapped the binding of HilD across the S. Typhimurium genome using chromatin immunoprecipitation-sequencing (ChIP-seq). Thus, we have identified 17 regions bound by HilD, including 11 novel targets. The majority of HilD targets are located outside SPI-1. We demonstrate transcription activation of 8 genes by HilD; four of these genes have not been previously described as being regulated by HilD, including lpxR, which encodes a lipid A deacylase important for immune evasion. We also show that HilD-activated genes are frequently activated by HilC and RtsA, indicating extensive overlap of the HilD, HilC, and RtsA regulons.

Authors+Show Affiliations

Wadsworth Center, New York State Department of Health, Albany, New York, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

24375101

Citation

Petrone, Brianna L., et al. "Identification of HilD-regulated Genes in Salmonella Enterica Serovar Typhimurium." Journal of Bacteriology, vol. 196, no. 5, 2014, pp. 1094-101.
Petrone BL, Stringer AM, Wade JT. Identification of HilD-regulated genes in Salmonella enterica serovar Typhimurium. J Bacteriol. 2014;196(5):1094-101.
Petrone, B. L., Stringer, A. M., & Wade, J. T. (2014). Identification of HilD-regulated genes in Salmonella enterica serovar Typhimurium. Journal of Bacteriology, 196(5), 1094-101. https://doi.org/10.1128/JB.01449-13
Petrone BL, Stringer AM, Wade JT. Identification of HilD-regulated Genes in Salmonella Enterica Serovar Typhimurium. J Bacteriol. 2014;196(5):1094-101. PubMed PMID: 24375101.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of HilD-regulated genes in Salmonella enterica serovar Typhimurium. AU - Petrone,Brianna L, AU - Stringer,Anne M, AU - Wade,Joseph T, Y1 - 2013/12/27/ PY - 2013/12/31/entrez PY - 2014/1/1/pubmed PY - 2014/4/8/medline SP - 1094 EP - 101 JF - Journal of bacteriology JO - J Bacteriol VL - 196 IS - 5 N2 - Salmonella enterica serovar Typhimurium (S. Typhimurium) pathogenicity island 1 (SPI-1) encodes a type III secretion system required for invasion of host gut epithelial cells. Expression of SPI-1 virulence genes is controlled by a complex hierarchy of transcription factors encoded within and outside SPI-1. The master regulator of SPI-1, HilA, is itself regulated by three homologous transcription factors, HilD, HilC, and RtsA. HilD activates transcription of hilA and other target genes in response to environmental conditions associated with the intestinal microenvironment of the host. We have mapped the binding of HilD across the S. Typhimurium genome using chromatin immunoprecipitation-sequencing (ChIP-seq). Thus, we have identified 17 regions bound by HilD, including 11 novel targets. The majority of HilD targets are located outside SPI-1. We demonstrate transcription activation of 8 genes by HilD; four of these genes have not been previously described as being regulated by HilD, including lpxR, which encodes a lipid A deacylase important for immune evasion. We also show that HilD-activated genes are frequently activated by HilC and RtsA, indicating extensive overlap of the HilD, HilC, and RtsA regulons. SN - 1098-5530 UR - https://www.unboundmedicine.com/medline/citation/24375101/Identification_of_HilD_regulated_genes_in_Salmonella_enterica_serovar_Typhimurium_ L2 - https://journals.asm.org/doi/10.1128/JB.01449-13?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -