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Screening and analysis of metabolites in rat urine after oral administration of Apocynum venetum L. extracts using HPLC-TOF-MS.
J Sep Sci. 2014 Mar; 37(5):515-26.JS

Abstract

HPLC with diode array detection and ESI-TOF-MS was used for the study of the constituents in Apocynum venetum L. extracts and the metabolites in rat urine after oral administration of A. venetum L. extracts. A formula database of the known constituents in A. venetum L. was established, and 21 constituents were rapidly identified by accurately matching their molecular masses with the formulae of the compounds in the database. Furthermore, 34 metabolites were detected and elucidated in the rat urine. The scientific and plausible biotransformation pathways of the flavonoid components in A. venetum L. were also proposed together with the presentation of clues for potential mechanisms of bioactivity. This specific and sensitive HPLC-ESI-TOF-MS method can be used to identify the chemical components in the extracts of A. venetum L. and their metabolites in rat urine. This method can also be used to reveal the possible metabolic mechanisms of action of the extract components in vivo.

Authors+Show Affiliations

Department of Pharmacy, Eastern Hepatobiliary Surgery Hospital, Shanghai, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

24376178

Citation

Zhao, Liang, et al. "Screening and Analysis of Metabolites in Rat Urine After Oral Administration of Apocynum Venetum L. Extracts Using HPLC-TOF-MS." Journal of Separation Science, vol. 37, no. 5, 2014, pp. 515-26.
Zhao L, Liang S, Lv L, et al. Screening and analysis of metabolites in rat urine after oral administration of Apocynum venetum L. extracts using HPLC-TOF-MS. J Sep Sci. 2014;37(5):515-26.
Zhao, L., Liang, S., Lv, L., Zhang, H., Guo-Tan, G., Chai, Y., & Zhang, G. (2014). Screening and analysis of metabolites in rat urine after oral administration of Apocynum venetum L. extracts using HPLC-TOF-MS. Journal of Separation Science, 37(5), 515-26. https://doi.org/10.1002/jssc.201301036
Zhao L, et al. Screening and Analysis of Metabolites in Rat Urine After Oral Administration of Apocynum Venetum L. Extracts Using HPLC-TOF-MS. J Sep Sci. 2014;37(5):515-26. PubMed PMID: 24376178.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Screening and analysis of metabolites in rat urine after oral administration of Apocynum venetum L. extracts using HPLC-TOF-MS. AU - Zhao,Liang, AU - Liang,Shanshan, AU - Lv,Lei, AU - Zhang,Hai, AU - Guo-Tan,Guang, AU - Chai,Yifeng, AU - Zhang,Guoqing, Y1 - 2014/01/22/ PY - 2013/09/21/received PY - 2013/12/11/revised PY - 2013/12/13/accepted PY - 2013/12/31/entrez PY - 2014/1/1/pubmed PY - 2014/10/21/medline KW - Apocynum venetum L KW - HPLC-TOF-MS KW - Identification KW - Metabolites KW - Rat urine SP - 515 EP - 26 JF - Journal of separation science JO - J Sep Sci VL - 37 IS - 5 N2 - HPLC with diode array detection and ESI-TOF-MS was used for the study of the constituents in Apocynum venetum L. extracts and the metabolites in rat urine after oral administration of A. venetum L. extracts. A formula database of the known constituents in A. venetum L. was established, and 21 constituents were rapidly identified by accurately matching their molecular masses with the formulae of the compounds in the database. Furthermore, 34 metabolites were detected and elucidated in the rat urine. The scientific and plausible biotransformation pathways of the flavonoid components in A. venetum L. were also proposed together with the presentation of clues for potential mechanisms of bioactivity. This specific and sensitive HPLC-ESI-TOF-MS method can be used to identify the chemical components in the extracts of A. venetum L. and their metabolites in rat urine. This method can also be used to reveal the possible metabolic mechanisms of action of the extract components in vivo. SN - 1615-9314 UR - https://www.unboundmedicine.com/medline/citation/24376178/Screening_and_analysis_of_metabolites_in_rat_urine_after_oral_administration_of_Apocynum_venetum_L__extracts_using_HPLC_TOF_MS_ L2 - https://doi.org/10.1002/jssc.201301036 DB - PRIME DP - Unbound Medicine ER -