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Comparative high-resolution mapping of the wax inhibitors Iw1 and Iw2 in hexaploid wheat.
PLoS One. 2013; 8(12):e84691.Plos

Abstract

The wax (glaucousness) on wheat leaves and stems is mainly controlled by two sets of genes: glaucousness loci (W1 and W2) and non-glaucousness loci (Iw1 and Iw2). The non-glaucousness (Iw) loci act as inhibitors of the glaucousness loci (W). High-resolution comparative genetic linkage maps of the wax inhibitors Iw1 originating from Triticum dicoccoides, and Iw2 from Aegilops tauschii were developed by comparative genomics analyses of Brachypodium, sorghum and rice genomic sequences corresponding to the syntenic regions of the Iw loci in wheat. Eleven Iw1 and eight Iw2 linked EST markers were developed and mapped to linkage maps on the distal regions of chromosomes 2BS and 2DS, respectively. The Iw1 locus mapped within a 0.96 cM interval flanked by the BE498358 and CA499581 EST markers that are collinear with 122 kb, 202 kb, and 466 kb genomic regions in the Brachypodium 5S chromosome, the sorghum 6S chromosome and the rice 4S chromosome, respectively. The Iw2 locus was located in a 4.1 to 5.4-cM interval in chromosome 2DS that is flanked by the CJ886319 and CJ519831 EST markers, and this region is collinear with a 2.3 cM region spanning the Iw1 locus on chromosome 2BS. Both Iw1 and Iw2 co-segregated with the BF474014 and CJ876545 EST markers, indicating they are most likely orthologs on 2BS and 2DS. These high-resolution maps can serve as a framework for chromosome landing, physical mapping and map-based cloning of the wax inhibitors in wheat.

Authors+Show Affiliations

State Key Laboratory for Agrobiotechnology / Beijing Key Laboratory of Crop Genetic Improvement / Key Laboratory of Crop Heterosis Research & Utilization, Department of Plant Genetics & Breeding, China Agricultural University, Beijing, China.State Key Laboratory for Agrobiotechnology / Beijing Key Laboratory of Crop Genetic Improvement / Key Laboratory of Crop Heterosis Research & Utilization, Department of Plant Genetics & Breeding, China Agricultural University, Beijing, China.State Key Laboratory for Agrobiotechnology / Beijing Key Laboratory of Crop Genetic Improvement / Key Laboratory of Crop Heterosis Research & Utilization, Department of Plant Genetics & Breeding, China Agricultural University, Beijing, China ; Plant Science and Technology College, Beijing University of Agriculture, Beijing, China.State Key Laboratory for Agrobiotechnology / Beijing Key Laboratory of Crop Genetic Improvement / Key Laboratory of Crop Heterosis Research & Utilization, Department of Plant Genetics & Breeding, China Agricultural University, Beijing, China.State Key Laboratory for Agrobiotechnology / Beijing Key Laboratory of Crop Genetic Improvement / Key Laboratory of Crop Heterosis Research & Utilization, Department of Plant Genetics & Breeding, China Agricultural University, Beijing, China.State Key Laboratory for Agrobiotechnology / Beijing Key Laboratory of Crop Genetic Improvement / Key Laboratory of Crop Heterosis Research & Utilization, Department of Plant Genetics & Breeding, China Agricultural University, Beijing, China.State Key Laboratory for Agrobiotechnology / Beijing Key Laboratory of Crop Genetic Improvement / Key Laboratory of Crop Heterosis Research & Utilization, Department of Plant Genetics & Breeding, China Agricultural University, Beijing, China.State Key Laboratory for Agrobiotechnology / Beijing Key Laboratory of Crop Genetic Improvement / Key Laboratory of Crop Heterosis Research & Utilization, Department of Plant Genetics & Breeding, China Agricultural University, Beijing, China.State Key Laboratory for Agrobiotechnology / Beijing Key Laboratory of Crop Genetic Improvement / Key Laboratory of Crop Heterosis Research & Utilization, Department of Plant Genetics & Breeding, China Agricultural University, Beijing, China.State Key Laboratory for Agrobiotechnology / Beijing Key Laboratory of Crop Genetic Improvement / Key Laboratory of Crop Heterosis Research & Utilization, Department of Plant Genetics & Breeding, China Agricultural University, Beijing, China.State Key Laboratory for Agrobiotechnology / Beijing Key Laboratory of Crop Genetic Improvement / Key Laboratory of Crop Heterosis Research & Utilization, Department of Plant Genetics & Breeding, China Agricultural University, Beijing, China.State Key Laboratory for Agrobiotechnology / Beijing Key Laboratory of Crop Genetic Improvement / Key Laboratory of Crop Heterosis Research & Utilization, Department of Plant Genetics & Breeding, China Agricultural University, Beijing, China.State Key Laboratory for Agrobiotechnology / Beijing Key Laboratory of Crop Genetic Improvement / Key Laboratory of Crop Heterosis Research & Utilization, Department of Plant Genetics & Breeding, China Agricultural University, Beijing, China.State Key Laboratory for Agrobiotechnology / Beijing Key Laboratory of Crop Genetic Improvement / Key Laboratory of Crop Heterosis Research & Utilization, Department of Plant Genetics & Breeding, China Agricultural University, Beijing, China.

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24376835

Citation

Wu, Haibin, et al. "Comparative High-resolution Mapping of the Wax Inhibitors Iw1 and Iw2 in Hexaploid Wheat." PloS One, vol. 8, no. 12, 2013, pp. e84691.
Wu H, Qin J, Han J, et al. Comparative high-resolution mapping of the wax inhibitors Iw1 and Iw2 in hexaploid wheat. PLoS One. 2013;8(12):e84691.
Wu, H., Qin, J., Han, J., Zhao, X., Ouyang, S., Liang, Y., Zhang, D., Wang, Z., Wu, Q., Xie, J., Cui, Y., Peng, H., Sun, Q., & Liu, Z. (2013). Comparative high-resolution mapping of the wax inhibitors Iw1 and Iw2 in hexaploid wheat. PloS One, 8(12), e84691. https://doi.org/10.1371/journal.pone.0084691
Wu H, et al. Comparative High-resolution Mapping of the Wax Inhibitors Iw1 and Iw2 in Hexaploid Wheat. PLoS One. 2013;8(12):e84691. PubMed PMID: 24376835.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative high-resolution mapping of the wax inhibitors Iw1 and Iw2 in hexaploid wheat. AU - Wu,Haibin, AU - Qin,Jinxia, AU - Han,Jun, AU - Zhao,Xiaojie, AU - Ouyang,Shuhong, AU - Liang,Yong, AU - Zhang,Dong, AU - Wang,Zhenzhong, AU - Wu,Qiuhong, AU - Xie,Jingzhong, AU - Cui,Yu, AU - Peng,Huiru, AU - Sun,Qixin, AU - Liu,Zhiyong, Y1 - 2013/12/23/ PY - 2013/04/06/received PY - 2013/11/25/accepted PY - 2013/12/31/entrez PY - 2014/1/1/pubmed PY - 2014/10/14/medline SP - e84691 EP - e84691 JF - PloS one JO - PLoS One VL - 8 IS - 12 N2 - The wax (glaucousness) on wheat leaves and stems is mainly controlled by two sets of genes: glaucousness loci (W1 and W2) and non-glaucousness loci (Iw1 and Iw2). The non-glaucousness (Iw) loci act as inhibitors of the glaucousness loci (W). High-resolution comparative genetic linkage maps of the wax inhibitors Iw1 originating from Triticum dicoccoides, and Iw2 from Aegilops tauschii were developed by comparative genomics analyses of Brachypodium, sorghum and rice genomic sequences corresponding to the syntenic regions of the Iw loci in wheat. Eleven Iw1 and eight Iw2 linked EST markers were developed and mapped to linkage maps on the distal regions of chromosomes 2BS and 2DS, respectively. The Iw1 locus mapped within a 0.96 cM interval flanked by the BE498358 and CA499581 EST markers that are collinear with 122 kb, 202 kb, and 466 kb genomic regions in the Brachypodium 5S chromosome, the sorghum 6S chromosome and the rice 4S chromosome, respectively. The Iw2 locus was located in a 4.1 to 5.4-cM interval in chromosome 2DS that is flanked by the CJ886319 and CJ519831 EST markers, and this region is collinear with a 2.3 cM region spanning the Iw1 locus on chromosome 2BS. Both Iw1 and Iw2 co-segregated with the BF474014 and CJ876545 EST markers, indicating they are most likely orthologs on 2BS and 2DS. These high-resolution maps can serve as a framework for chromosome landing, physical mapping and map-based cloning of the wax inhibitors in wheat. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/24376835/Comparative_high_resolution_mapping_of_the_wax_inhibitors_Iw1_and_Iw2_in_hexaploid_wheat_ L2 - https://dx.plos.org/10.1371/journal.pone.0084691 DB - PRIME DP - Unbound Medicine ER -