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Oleuropein induces apoptosis via the p53 pathway in breast cancer cells.

Abstract

BACKGROUND

Breast cancer is a major health problem worldwide. Olive oil induces apoptosis in some cancer cells due to phenolic compounds like oleuropein. Although oleuropein has anticancer activity, the underlying mechanisms of action remain unknown. The study aimed to assess the mechanism of oleuropin-induced breast cancer cell apoptosis.

MATERIALS AND METHODS

p53, Bcl-2 and Bax gene expression was evaluated by semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) in luminal MCF-7 cells.

RESULTS

Oleuropein-induced apoptosis was accompanied by up-regulation of both p53 and Bax gene expression levels and down-regulation in Bcl2.

CONCLUSIONS

Oleuropein induces apoptosis in breast tumour cells via a p53-dependent pathway mediated by Bax and Bcl2 genes. Therefore, oleuropein may have therapeutic potential in breast cancer patients by inducing apoptosis via activation of the p53 pathway.

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  • Authors+Show Affiliations

    ,

    Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia E-mail : hildahafez@hotmail.com.

    , , , , ,

    Source

    MeSH

    Antihypertensive Agents
    Blotting, Western
    Breast Neoplasms
    Cell Proliferation
    Female
    Gene Expression Regulation, Neoplastic
    Humans
    Iridoids
    Proto-Oncogene Proteins c-bcl-2
    RNA, Messenger
    Real-Time Polymerase Chain Reaction
    Reverse Transcriptase Polymerase Chain Reaction
    Tumor Cells, Cultured
    Tumor Suppressor Protein p53
    bcl-2-Associated X Protein

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    24377598

    Citation

    Hassan, Zeinab Korany, et al. "Oleuropein Induces Apoptosis Via the P53 Pathway in Breast Cancer Cells." Asian Pacific Journal of Cancer Prevention : APJCP, vol. 14, no. 11, 2014, pp. 6739-42.
    Hassan ZK, Elamin MH, Omer SA, et al. Oleuropein induces apoptosis via the p53 pathway in breast cancer cells. Asian Pac J Cancer Prev. 2014;14(11):6739-42.
    Hassan, Z. K., Elamin, M. H., Omer, S. A., Daghestani, M. H., Al-Olayan, E. S., Elobeid, M. A., & Virk, P. (2014). Oleuropein induces apoptosis via the p53 pathway in breast cancer cells. Asian Pacific Journal of Cancer Prevention : APJCP, 14(11), pp. 6739-42.
    Hassan ZK, et al. Oleuropein Induces Apoptosis Via the P53 Pathway in Breast Cancer Cells. Asian Pac J Cancer Prev. 2014;14(11):6739-42. PubMed PMID: 24377598.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Oleuropein induces apoptosis via the p53 pathway in breast cancer cells. AU - Hassan,Zeinab Korany, AU - Elamin,Maha Hussein, AU - Omer,Sawsan Ali, AU - Daghestani,Maha Hassan, AU - Al-Olayan,Ebtesam Salah, AU - Elobeid,Mai Abdelrahman, AU - Virk,Promy, PY - 2014/1/1/entrez PY - 2014/1/1/pubmed PY - 2015/1/7/medline SP - 6739 EP - 42 JF - Asian Pacific journal of cancer prevention : APJCP JO - Asian Pac. J. Cancer Prev. VL - 14 IS - 11 N2 - BACKGROUND: Breast cancer is a major health problem worldwide. Olive oil induces apoptosis in some cancer cells due to phenolic compounds like oleuropein. Although oleuropein has anticancer activity, the underlying mechanisms of action remain unknown. The study aimed to assess the mechanism of oleuropin-induced breast cancer cell apoptosis. MATERIALS AND METHODS: p53, Bcl-2 and Bax gene expression was evaluated by semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) in luminal MCF-7 cells. RESULTS: Oleuropein-induced apoptosis was accompanied by up-regulation of both p53 and Bax gene expression levels and down-regulation in Bcl2. CONCLUSIONS: Oleuropein induces apoptosis in breast tumour cells via a p53-dependent pathway mediated by Bax and Bcl2 genes. Therefore, oleuropein may have therapeutic potential in breast cancer patients by inducing apoptosis via activation of the p53 pathway. SN - 2476-762X UR - https://www.unboundmedicine.com/medline/citation/24377598/Oleuropein_induces_apoptosis_via_the_p53_pathway_in_breast_cancer_cells_ L2 - http://journal.waocp.org/?sid=Entrez:PubMed&id=pmid:24377598&key=2014.14.11.6739 DB - PRIME DP - Unbound Medicine ER -