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Paraspecificity of Vipera a. ammodytes-specific antivenom towards Montivipera raddei and Macrovipera lebetina obtusa venoms.
Toxicon. 2014 Feb; 78:103-12.T

Abstract

Antivenom raised against the venom of nose-horned viper, Vipera ammodytes (V. a.) ammodytes (European viper venom antiserum, Zagreb antivenom), contains neutralising equine F(ab')₂ fragments that are clinically successful against homologous venom, but also against the venoms of several others medically important European snakes due to its paraspecific action. In this work we demonstrated that Zagreb antivenom is preclinically effective in neutralising lethal toxicity and hemorrhagicity of venoms of Armenian mountain snakes--Montivipera raddei and Macrovipera lebetina obtusa as well. In order to better understand the biochemical basis of the observed paraspecificity, the ability of anti-V. a. ammodytes serum to recognise and neutralise proteinases of the two venoms was also investigated. Anti-V. a. ammodytes serum showed surprisingly low capacity to inhibit metalloproteinases of both venoms included in the study, probably due to weak immunorecognition of their P-I representatives. Also, it completely failed to abolish enzymatic action of serine proteinases from Macrovipera lebetina obtusa venom. Relevance of such finding is yet to be established.

Authors+Show Affiliations

University of Zagreb, Centre for Research and Knowledge Transfer in Biotechnology, Rockefellerova 10, 10 000 Zagreb, Croatia.Institute of Immunology Inc., Rockefellerova 10, 10 000 Zagreb, Croatia.Orbeli Institute of Physiology, National Academy of Sciences, 22, Orbeli Bros. str., Yerevan 0028, Armenia.University of Zagreb, Centre for Research and Knowledge Transfer in Biotechnology, Rockefellerova 10, 10 000 Zagreb, Croatia. Electronic address: bhalassy@unizg.hr.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24378834

Citation

Kurtović, Tihana, et al. "Paraspecificity of Vipera A. Ammodytes-specific Antivenom Towards Montivipera Raddei and Macrovipera Lebetina Obtusa Venoms." Toxicon : Official Journal of the International Society On Toxinology, vol. 78, 2014, pp. 103-12.
Kurtović T, Lang Balija M, Ayvazyan N, et al. Paraspecificity of Vipera a. ammodytes-specific antivenom towards Montivipera raddei and Macrovipera lebetina obtusa venoms. Toxicon. 2014;78:103-12.
Kurtović, T., Lang Balija, M., Ayvazyan, N., & Halassy, B. (2014). Paraspecificity of Vipera a. ammodytes-specific antivenom towards Montivipera raddei and Macrovipera lebetina obtusa venoms. Toxicon : Official Journal of the International Society On Toxinology, 78, 103-12. https://doi.org/10.1016/j.toxicon.2013.12.004
Kurtović T, et al. Paraspecificity of Vipera A. Ammodytes-specific Antivenom Towards Montivipera Raddei and Macrovipera Lebetina Obtusa Venoms. Toxicon. 2014;78:103-12. PubMed PMID: 24378834.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Paraspecificity of Vipera a. ammodytes-specific antivenom towards Montivipera raddei and Macrovipera lebetina obtusa venoms. AU - Kurtović,Tihana, AU - Lang Balija,Maja, AU - Ayvazyan,Naira, AU - Halassy,Beata, Y1 - 2013/12/27/ PY - 2013/10/28/received PY - 2013/12/02/revised PY - 2013/12/19/accepted PY - 2014/1/1/entrez PY - 2014/1/1/pubmed PY - 2014/9/27/medline KW - Macrovipera lebetina obtusa KW - Montivipera raddei KW - Paraspecificity KW - Snake venom metalloproteinases KW - Snake venom serine proteinases KW - Zagreb antivenom SP - 103 EP - 12 JF - Toxicon : official journal of the International Society on Toxinology JO - Toxicon VL - 78 N2 - Antivenom raised against the venom of nose-horned viper, Vipera ammodytes (V. a.) ammodytes (European viper venom antiserum, Zagreb antivenom), contains neutralising equine F(ab')₂ fragments that are clinically successful against homologous venom, but also against the venoms of several others medically important European snakes due to its paraspecific action. In this work we demonstrated that Zagreb antivenom is preclinically effective in neutralising lethal toxicity and hemorrhagicity of venoms of Armenian mountain snakes--Montivipera raddei and Macrovipera lebetina obtusa as well. In order to better understand the biochemical basis of the observed paraspecificity, the ability of anti-V. a. ammodytes serum to recognise and neutralise proteinases of the two venoms was also investigated. Anti-V. a. ammodytes serum showed surprisingly low capacity to inhibit metalloproteinases of both venoms included in the study, probably due to weak immunorecognition of their P-I representatives. Also, it completely failed to abolish enzymatic action of serine proteinases from Macrovipera lebetina obtusa venom. Relevance of such finding is yet to be established. SN - 1879-3150 UR - https://www.unboundmedicine.com/medline/citation/24378834/Paraspecificity_of_Vipera_a__ammodytes_specific_antivenom_towards_Montivipera_raddei_and_Macrovipera_lebetina_obtusa_venoms_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0041-0101(13)00460-1 DB - PRIME DP - Unbound Medicine ER -