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Identification of the self-incompatibility locus F-box protein-containing complex in Petunia inflata.
Plant Reprod. 2014 Mar; 27(1):31-45.PR

Abstract

The polymorphic S-locus regulating self-incompatibility (SI) in Petunia contains the S-RNase gene and a number of S-locus F-box (SLF) genes. While penetrating the style through the stigma, a pollen tube takes up all S-RNases, but only self S-RNase inhibits pollen tube growth. Recent evidence suggests that SLFs produced by pollen collectively interact with and detoxify non-self S-RNases, but none can interact with self S-RNase. An SLF may be the F-box protein component of an SCF complex (containing Cullin1, Skp1 and Rbx1), which mediates ubiquitination of protein substrates for degradation by the 26S proteasome. However, the precise nature of the complex is unknown. We used pollen extracts of a transgenic plant over-expressing GFP-fused S2-SLF1 (SLF1 of S 2-haplotype) for co-immunoprecipitation (Co-IP) followed by mass spectrometry (MS). We identified PiCUL1-P (a pollen-specific Cullin1), PiSSK1 (a pollen-specific Skp1-like protein) and PiRBX1 (an Rbx1). To validate the results, we raised transgenic plants over-expressing PiSSK1:FLAG:GFP and used pollen extracts for Co-IP-MS. The results confirmed the presence of PiCUL1-P and PiRBX1 in the complex and identified two different SLFs as the F-box protein component. Thus, all but Rbx1 of the complex may have evolved in SI, and all SLFs may be the F-box component of similar complexes.

Authors+Show Affiliations

Intercollege Graduate Degree Program in Plant Biology, Pennsylvania State University, University Park, PA, 16802, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

24381071

Citation

Li, Shu, et al. "Identification of the Self-incompatibility Locus F-box Protein-containing Complex in Petunia Inflata." Plant Reproduction, vol. 27, no. 1, 2014, pp. 31-45.
Li S, Sun P, Williams JS, et al. Identification of the self-incompatibility locus F-box protein-containing complex in Petunia inflata. Plant Reprod. 2014;27(1):31-45.
Li, S., Sun, P., Williams, J. S., & Kao, T. H. (2014). Identification of the self-incompatibility locus F-box protein-containing complex in Petunia inflata. Plant Reproduction, 27(1), 31-45. https://doi.org/10.1007/s00497-013-0238-3
Li S, et al. Identification of the Self-incompatibility Locus F-box Protein-containing Complex in Petunia Inflata. Plant Reprod. 2014;27(1):31-45. PubMed PMID: 24381071.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of the self-incompatibility locus F-box protein-containing complex in Petunia inflata. AU - Li,Shu, AU - Sun,Penglin, AU - Williams,Justin Stephen, AU - Kao,Teh-hui, Y1 - 2014/01/01/ PY - 2013/10/01/received PY - 2013/12/17/accepted PY - 2014/1/2/entrez PY - 2014/1/2/pubmed PY - 2014/9/24/medline SP - 31 EP - 45 JF - Plant reproduction JO - Plant Reprod VL - 27 IS - 1 N2 - The polymorphic S-locus regulating self-incompatibility (SI) in Petunia contains the S-RNase gene and a number of S-locus F-box (SLF) genes. While penetrating the style through the stigma, a pollen tube takes up all S-RNases, but only self S-RNase inhibits pollen tube growth. Recent evidence suggests that SLFs produced by pollen collectively interact with and detoxify non-self S-RNases, but none can interact with self S-RNase. An SLF may be the F-box protein component of an SCF complex (containing Cullin1, Skp1 and Rbx1), which mediates ubiquitination of protein substrates for degradation by the 26S proteasome. However, the precise nature of the complex is unknown. We used pollen extracts of a transgenic plant over-expressing GFP-fused S2-SLF1 (SLF1 of S 2-haplotype) for co-immunoprecipitation (Co-IP) followed by mass spectrometry (MS). We identified PiCUL1-P (a pollen-specific Cullin1), PiSSK1 (a pollen-specific Skp1-like protein) and PiRBX1 (an Rbx1). To validate the results, we raised transgenic plants over-expressing PiSSK1:FLAG:GFP and used pollen extracts for Co-IP-MS. The results confirmed the presence of PiCUL1-P and PiRBX1 in the complex and identified two different SLFs as the F-box protein component. Thus, all but Rbx1 of the complex may have evolved in SI, and all SLFs may be the F-box component of similar complexes. SN - 2194-7961 UR - https://www.unboundmedicine.com/medline/citation/24381071/Identification_of_the_self_incompatibility_locus_F_box_protein_containing_complex_in_Petunia_inflata_ DB - PRIME DP - Unbound Medicine ER -