Tags

Type your tag names separated by a space and hit enter

Heat and AITC activate green anole TRPA1 in a membrane-delimited manner.
Pflugers Arch. 2014 Oct; 466(10):1873-84.PA

Abstract

Transient receptor potential ankyrin 1 (TRPA1) is a member of the large TRP super family of ion channels and functions as a Ca(2+)-permeable nonselective cation channel that is activated by various noxious stimuli. TRPA1 was initially identified as a potential mediator of noxious cold stimuli in mammalian nociceptive sensory neurons, while TRPA1s from nonmammalian vertebrates (snakes, green anole lizards, and frogs) were recently reported to be activated by heat, but not cold stimulus. In this study, we examined detailed properties of the green anole TRPA1 channel (gaTRPA1) related to thermal and chemical stimulation in whole-cell and single-channel recordings. Heat activates gaTRPA1 with a temperature threshold for activation of 35.8 °C, while heat together with allyl isothiocyanate (AITC), a chemical agonist, had synergistic effects on gaTRPA1 channel activation in that either the temperature threshold or activating AITC concentration was reduced in the presence of the other stimulus. Significant heat-evoked gaTRPA1 activation was observed in the presence but not absence of extracellular Ca(2+). gaTRPA1 channels were also activated by heat and AITC in excised membrane patches with an inside-out configuration. By comparing the kinetics of heat- and AITC-evoked single-channel currents, we defined similarities and differences of gaTRPA1 channel responses to heat and AITC. We observed similar current-voltage relationship and unitary amplitudes for heat- and AITC-evoked currents and found that heat-activated currents showed shorter durations of both open and closed times. Our results suggest that the gaTRPA1 channel is directly activated by heat and chemical stimuli.

Authors+Show Affiliations

Division of Cell Signaling, Okazaki Institute for Integrative Bioscience (National Institute for Physiological Sciences), National Institutes of Natural Sciences, Higashiyama 5-1, Myodaiji, Okazaki, 444-8787, Japan.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24385018

Citation

Kurganov, Erkin, et al. "Heat and AITC Activate Green Anole TRPA1 in a Membrane-delimited Manner." Pflugers Archiv : European Journal of Physiology, vol. 466, no. 10, 2014, pp. 1873-84.
Kurganov E, Zhou Y, Saito S, et al. Heat and AITC activate green anole TRPA1 in a membrane-delimited manner. Pflugers Arch. 2014;466(10):1873-84.
Kurganov, E., Zhou, Y., Saito, S., & Tominaga, M. (2014). Heat and AITC activate green anole TRPA1 in a membrane-delimited manner. Pflugers Archiv : European Journal of Physiology, 466(10), 1873-84. https://doi.org/10.1007/s00424-013-1420-z
Kurganov E, et al. Heat and AITC Activate Green Anole TRPA1 in a Membrane-delimited Manner. Pflugers Arch. 2014;466(10):1873-84. PubMed PMID: 24385018.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Heat and AITC activate green anole TRPA1 in a membrane-delimited manner. AU - Kurganov,Erkin, AU - Zhou,Yiming, AU - Saito,Shigeru, AU - Tominaga,Makoto, Y1 - 2014/01/03/ PY - 2013/09/01/received PY - 2013/12/03/accepted PY - 2013/12/03/revised PY - 2014/1/4/entrez PY - 2014/1/5/pubmed PY - 2015/5/23/medline SP - 1873 EP - 84 JF - Pflugers Archiv : European journal of physiology JO - Pflugers Arch VL - 466 IS - 10 N2 - Transient receptor potential ankyrin 1 (TRPA1) is a member of the large TRP super family of ion channels and functions as a Ca(2+)-permeable nonselective cation channel that is activated by various noxious stimuli. TRPA1 was initially identified as a potential mediator of noxious cold stimuli in mammalian nociceptive sensory neurons, while TRPA1s from nonmammalian vertebrates (snakes, green anole lizards, and frogs) were recently reported to be activated by heat, but not cold stimulus. In this study, we examined detailed properties of the green anole TRPA1 channel (gaTRPA1) related to thermal and chemical stimulation in whole-cell and single-channel recordings. Heat activates gaTRPA1 with a temperature threshold for activation of 35.8 °C, while heat together with allyl isothiocyanate (AITC), a chemical agonist, had synergistic effects on gaTRPA1 channel activation in that either the temperature threshold or activating AITC concentration was reduced in the presence of the other stimulus. Significant heat-evoked gaTRPA1 activation was observed in the presence but not absence of extracellular Ca(2+). gaTRPA1 channels were also activated by heat and AITC in excised membrane patches with an inside-out configuration. By comparing the kinetics of heat- and AITC-evoked single-channel currents, we defined similarities and differences of gaTRPA1 channel responses to heat and AITC. We observed similar current-voltage relationship and unitary amplitudes for heat- and AITC-evoked currents and found that heat-activated currents showed shorter durations of both open and closed times. Our results suggest that the gaTRPA1 channel is directly activated by heat and chemical stimuli. SN - 1432-2013 UR - https://www.unboundmedicine.com/medline/citation/24385018/Heat_and_AITC_activate_green_anole_TRPA1_in_a_membrane_delimited_manner_ DB - PRIME DP - Unbound Medicine ER -