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Population structure and virulence content of avian pathogenic Escherichia coli isolated from outbreaks in Sri Lanka.
Vet Microbiol. 2014 Jan 31; 168(2-4):403-12.VM

Abstract

Avian pathogenic Escherichia coli (APEC) causes economically significant infections in poultry. The genetic diversity of APEC and phylogenetic relationships within and between APEC and other pathogenic E. coli are not yet well understood. We used multilocus sequence typing (MLST), PCR-based phylogrouping and virulence genotyping to analyse 75 avian E. coli strains, including 55 isolated from outbreaks of colisepticaemia and 20 from healthy chickens. Isolates were collected from 42 commercial layer and broiler chicken farms in Sri Lanka. MLST identified 61 sequence types (ST) with 44 being novel. The most frequent ST, ST48, was represented by only six isolates followed by ST117 with four isolates. Phylogenetic clusters based on MLST sequences were mostly comparable to phylogrouping by PCR and MLST further differentiated phylogroups B1 and D into two subgroups. Genotyping of 16 APEC associated virulence genes found that 27 of the clinical isolates and one isolate from a healthy chicken belonged to highly virulent genotype according to previously established classification schemes. We found that a combination of four genes, ompT, hlyF, iroN and papC, gave a comparable prediction to that of using five and nine genes by other studies. Four STs (ST10, ST48, ST117 and ST2016) contained APEC isolates from this study and human UPEC isolates reported by others, suggesting that these STs are potentially zoonotic. Our results enhanced the understanding of APEC population structure and virulence association.

Authors+Show Affiliations

School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia.School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia.School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia. Electronic address: r.lan@unsw.edu.au.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24388626

Citation

Dissanayake, D R A., et al. "Population Structure and Virulence Content of Avian Pathogenic Escherichia Coli Isolated From Outbreaks in Sri Lanka." Veterinary Microbiology, vol. 168, no. 2-4, 2014, pp. 403-12.
Dissanayake DR, Octavia S, Lan R. Population structure and virulence content of avian pathogenic Escherichia coli isolated from outbreaks in Sri Lanka. Vet Microbiol. 2014;168(2-4):403-12.
Dissanayake, D. R., Octavia, S., & Lan, R. (2014). Population structure and virulence content of avian pathogenic Escherichia coli isolated from outbreaks in Sri Lanka. Veterinary Microbiology, 168(2-4), 403-12. https://doi.org/10.1016/j.vetmic.2013.11.028
Dissanayake DR, Octavia S, Lan R. Population Structure and Virulence Content of Avian Pathogenic Escherichia Coli Isolated From Outbreaks in Sri Lanka. Vet Microbiol. 2014 Jan 31;168(2-4):403-12. PubMed PMID: 24388626.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Population structure and virulence content of avian pathogenic Escherichia coli isolated from outbreaks in Sri Lanka. AU - Dissanayake,D R A, AU - Octavia,Sophie, AU - Lan,Ruiting, Y1 - 2013/12/11/ PY - 2013/02/20/received PY - 2013/10/27/revised PY - 2013/11/22/accepted PY - 2014/1/7/entrez PY - 2014/1/7/pubmed PY - 2014/5/10/medline KW - APEC KW - MLST KW - Phylogroup KW - Virulence factors SP - 403 EP - 12 JF - Veterinary microbiology JO - Vet Microbiol VL - 168 IS - 2-4 N2 - Avian pathogenic Escherichia coli (APEC) causes economically significant infections in poultry. The genetic diversity of APEC and phylogenetic relationships within and between APEC and other pathogenic E. coli are not yet well understood. We used multilocus sequence typing (MLST), PCR-based phylogrouping and virulence genotyping to analyse 75 avian E. coli strains, including 55 isolated from outbreaks of colisepticaemia and 20 from healthy chickens. Isolates were collected from 42 commercial layer and broiler chicken farms in Sri Lanka. MLST identified 61 sequence types (ST) with 44 being novel. The most frequent ST, ST48, was represented by only six isolates followed by ST117 with four isolates. Phylogenetic clusters based on MLST sequences were mostly comparable to phylogrouping by PCR and MLST further differentiated phylogroups B1 and D into two subgroups. Genotyping of 16 APEC associated virulence genes found that 27 of the clinical isolates and one isolate from a healthy chicken belonged to highly virulent genotype according to previously established classification schemes. We found that a combination of four genes, ompT, hlyF, iroN and papC, gave a comparable prediction to that of using five and nine genes by other studies. Four STs (ST10, ST48, ST117 and ST2016) contained APEC isolates from this study and human UPEC isolates reported by others, suggesting that these STs are potentially zoonotic. Our results enhanced the understanding of APEC population structure and virulence association. SN - 1873-2542 UR - https://www.unboundmedicine.com/medline/citation/24388626/Population_structure_and_virulence_content_of_avian_pathogenic_Escherichia_coli_isolated_from_outbreaks_in_Sri_Lanka_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-1135(13)00552-X DB - PRIME DP - Unbound Medicine ER -