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NS1 antigen testing for the diagnosis of dengue in returned Israeli travelers.
J Med Virol. 2014 Dec; 86(12):2005-10.JM

Abstract

Antigen testing with NS1 provides rapid diagnosis of dengue in endemic regions during the febrile phase of illness before appearance of IgM in serum. This study aimed to determine the diagnostic accuracy of NS1 antigen testing in travelers presenting with febrile illness and serologically confirmed dengue infection, upon return from dengue endemic countries to Israel, a region endemic for West Nile virus (WNV). Cases were sera obtained from febrile returning travelers with positive dengue-IgM antibodies. Sera of non-travelers with confirmed WNV disease and sera of returning travelers with confirmed non-dengue febrile illnesses were used as controls. All sera were tested for NS1 antigen using the Panbio Dengue Early ELISA assay within 21 days of symptoms. Demographic data, travel destination, and interval between disease onset and testing were retrieved from patient files. Fifty-eight sera from 40 dengue-infected travelers, 26 sera from 26 WNV- infected patients, and 15 sera of returning travelers with non-dengue febrile illness were tested. Sensitivity of NS1 testing in dengue patients was 87% during the first 3 days of symptoms and declined to about 70% after 12 days. No cases tested positive for NS1 after day 12. Specificity was 92% for the entire testing period. The NS1 Panbio assay is sensitive for the detection of dengue viral infection in returning travelers during the febrile phase of illness, and is highly specific in a region where WNV co-circulates.

Authors+Show Affiliations

The Pediatric Infectious Disease Unit, Soroka University Medical Center, Ben-Gurion University of the Negev, Beer-Sheva, Israel.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

24390973

Citation

Fuchs, Inbal, et al. "NS1 Antigen Testing for the Diagnosis of Dengue in Returned Israeli Travelers." Journal of Medical Virology, vol. 86, no. 12, 2014, pp. 2005-10.
Fuchs I, Bin H, Schlezinger S, et al. NS1 antigen testing for the diagnosis of dengue in returned Israeli travelers. J Med Virol. 2014;86(12):2005-10.
Fuchs, I., Bin, H., Schlezinger, S., & Schwartz, E. (2014). NS1 antigen testing for the diagnosis of dengue in returned Israeli travelers. Journal of Medical Virology, 86(12), 2005-10. https://doi.org/10.1002/jmv.23879
Fuchs I, et al. NS1 Antigen Testing for the Diagnosis of Dengue in Returned Israeli Travelers. J Med Virol. 2014;86(12):2005-10. PubMed PMID: 24390973.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - NS1 antigen testing for the diagnosis of dengue in returned Israeli travelers. AU - Fuchs,Inbal, AU - Bin,Hana, AU - Schlezinger,Sara, AU - Schwartz,Eli, Y1 - 2014/01/06/ PY - 2013/12/12/accepted PY - 2014/1/7/entrez PY - 2014/1/7/pubmed PY - 2015/6/19/medline KW - dengue KW - diagnosis KW - non-structural protein 1 KW - traveler SP - 2005 EP - 10 JF - Journal of medical virology JO - J Med Virol VL - 86 IS - 12 N2 - Antigen testing with NS1 provides rapid diagnosis of dengue in endemic regions during the febrile phase of illness before appearance of IgM in serum. This study aimed to determine the diagnostic accuracy of NS1 antigen testing in travelers presenting with febrile illness and serologically confirmed dengue infection, upon return from dengue endemic countries to Israel, a region endemic for West Nile virus (WNV). Cases were sera obtained from febrile returning travelers with positive dengue-IgM antibodies. Sera of non-travelers with confirmed WNV disease and sera of returning travelers with confirmed non-dengue febrile illnesses were used as controls. All sera were tested for NS1 antigen using the Panbio Dengue Early ELISA assay within 21 days of symptoms. Demographic data, travel destination, and interval between disease onset and testing were retrieved from patient files. Fifty-eight sera from 40 dengue-infected travelers, 26 sera from 26 WNV- infected patients, and 15 sera of returning travelers with non-dengue febrile illness were tested. Sensitivity of NS1 testing in dengue patients was 87% during the first 3 days of symptoms and declined to about 70% after 12 days. No cases tested positive for NS1 after day 12. Specificity was 92% for the entire testing period. The NS1 Panbio assay is sensitive for the detection of dengue viral infection in returning travelers during the febrile phase of illness, and is highly specific in a region where WNV co-circulates. SN - 1096-9071 UR - https://www.unboundmedicine.com/medline/citation/24390973/NS1_antigen_testing_for_the_diagnosis_of_dengue_in_returned_Israeli_travelers_ L2 - https://doi.org/10.1002/jmv.23879 DB - PRIME DP - Unbound Medicine ER -