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[Novel gene transfer using micellar nanovectors inhibits choroidal neovascularization].
Nippon Ganka Gakkai Zasshi. 2013 Nov; 117(11):869-77.NG

Abstract

The treatment of age-related macular degeneration (AMD) caused by choroidal neovascularization (CNV) is difficult. More effective therapy for regulating CNV is needed. We demonstrated that intravenous nonviral vectors based on the complex of plasmid DNA with synthetic cationic polymers accumulate in choroidal neovascularization (CNV) with high efficiency through an enhanced the permeability and retention (EPR) effect. This review shows the results of in vivo angiogenic control by intravenous injection of a polyplex micelle-encapsulating plasmid vector using a mice CNV model. Polyion complex (PIC) micelles consisting of plasmid DNA and poly (ethylene glycol)-b-poly (N-[N-(2-aminoethyl)-2-aminoethyl] aspartamidef block copolymers [PEG-b-PAsp (DET)] were used. These show minimal cytotoxicity and high transfection efficiency both in vitro and in vivo, and have been utilized for gene therapy against a mouse corneal neovascularization model by local administration of plasmid-encoding soluble vascular endothelial growth factor receptor 1 (soluble Fms-like tyrosine kinase-1: sFlt-1). Transfection of plasmid-expressing sFlt-1 with PEG-C6-P[Asp (DET)] polyplex micelles by intravenous injection into mice CNV models showed significant inhibition of developing CNV. We found that nonviral gene therapy has significant potential for regulation of CNV using plasmids with PEG-C6-P [Asp (DET)] polyplex micelles.

Authors+Show Affiliations

Department of Ophthalmology, Tokyo Metropolitan Geriatric Hospital, Tokyo 173-0015, Japan. akagi-tky@umin.ac.jp

Pub Type(s)

English Abstract
Journal Article
Review

Language

jpn

PubMed ID

24397183

Citation

Aoki, Aya. "[Novel Gene Transfer Using Micellar Nanovectors Inhibits Choroidal Neovascularization]." Nippon Ganka Gakkai Zasshi, vol. 117, no. 11, 2013, pp. 869-77.
Aoki A. [Novel gene transfer using micellar nanovectors inhibits choroidal neovascularization]. Nippon Ganka Gakkai Zasshi. 2013;117(11):869-77.
Aoki, A. (2013). [Novel gene transfer using micellar nanovectors inhibits choroidal neovascularization]. Nippon Ganka Gakkai Zasshi, 117(11), 869-77.
Aoki A. [Novel Gene Transfer Using Micellar Nanovectors Inhibits Choroidal Neovascularization]. Nippon Ganka Gakkai Zasshi. 2013;117(11):869-77. PubMed PMID: 24397183.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Novel gene transfer using micellar nanovectors inhibits choroidal neovascularization]. A1 - Aoki,Aya, PY - 2014/1/9/entrez PY - 2014/1/9/pubmed PY - 2014/2/5/medline SP - 869 EP - 77 JF - Nippon Ganka Gakkai zasshi JO - Nippon Ganka Gakkai Zasshi VL - 117 IS - 11 N2 - The treatment of age-related macular degeneration (AMD) caused by choroidal neovascularization (CNV) is difficult. More effective therapy for regulating CNV is needed. We demonstrated that intravenous nonviral vectors based on the complex of plasmid DNA with synthetic cationic polymers accumulate in choroidal neovascularization (CNV) with high efficiency through an enhanced the permeability and retention (EPR) effect. This review shows the results of in vivo angiogenic control by intravenous injection of a polyplex micelle-encapsulating plasmid vector using a mice CNV model. Polyion complex (PIC) micelles consisting of plasmid DNA and poly (ethylene glycol)-b-poly (N-[N-(2-aminoethyl)-2-aminoethyl] aspartamidef block copolymers [PEG-b-PAsp (DET)] were used. These show minimal cytotoxicity and high transfection efficiency both in vitro and in vivo, and have been utilized for gene therapy against a mouse corneal neovascularization model by local administration of plasmid-encoding soluble vascular endothelial growth factor receptor 1 (soluble Fms-like tyrosine kinase-1: sFlt-1). Transfection of plasmid-expressing sFlt-1 with PEG-C6-P[Asp (DET)] polyplex micelles by intravenous injection into mice CNV models showed significant inhibition of developing CNV. We found that nonviral gene therapy has significant potential for regulation of CNV using plasmids with PEG-C6-P [Asp (DET)] polyplex micelles. SN - 0029-0203 UR - https://www.unboundmedicine.com/medline/citation/24397183/[Novel_gene_transfer_using_micellar_nanovectors_inhibits_choroidal_neovascularization]_ L2 - http://www.medicalonline.jp/meteo_linkout.php?issn=0029-0203&volume=117&issue=11&spage=869 DB - PRIME DP - Unbound Medicine ER -