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3D conductive nanocomposite scaffold for bone tissue engineering.
Int J Nanomedicine. 2014; 9:167-81.IJ

Abstract

Bone healing can be significantly expedited by applying electrical stimuli in the injured region. Therefore, a three-dimensional (3D) ceramic conductive tissue engineering scaffold for large bone defects that can locally deliver the electrical stimuli is highly desired. In the present study, 3D conductive scaffolds were prepared by employing a biocompatible conductive polymer, ie, poly(3,4-ethylenedioxythiophene) poly(4-styrene sulfonate) (PEDOT:PSS), in the optimized nanocomposite of gelatin and bioactive glass. For in vitro analysis, adult human mesenchymal stem cells were seeded in the scaffolds. Material characterizations using hydrogen-1 nuclear magnetic resonance, in vitro degradation, as well as thermal and mechanical analysis showed that incorporation of PEDOT:PSS increased the physiochemical stability of the composite, resulting in improved mechanical properties and biodegradation resistance. The outcomes indicate that PEDOT:PSS and polypeptide chains have close interaction, most likely by forming salt bridges between arginine side chains and sulfonate groups. The morphology of the scaffolds and cultured human mesenchymal stem cells were observed and analyzed via scanning electron microscope, micro-computed tomography, and confocal fluorescent microscope. Increasing the concentration of the conductive polymer in the scaffold enhanced the cell viability, indicating the improved microstructure of the scaffolds or boosted electrical signaling among cells. These results show that these conductive scaffolds are not only structurally more favorable for bone tissue engineering, but also can be a step forward in combining the tissue engineering techniques with the method of enhancing the bone healing by electrical stimuli.

Authors+Show Affiliations

School of Electrical and Computer Engineering, Helmerich Advanced Technology Research Center, Oklahoma State University, Stillwater, OK, USA.School of Chemical Engineering, Oklahoma State University, Stillwater, OK, USA.School of Chemical Engineering, Oklahoma State University, Stillwater, OK, USA.Department of Chemistry, Oklahoma State University, Stillwater, OK, USA.School of Mechanical and Aerospace Engineering, Oklahoma State University, Stillwater, OK, USA.Department of Nutritional Sciences, Oklahoma State University, Stillwater, OK, USA.Department of Biomaterials and Biomimetics, New York University, New York, NY.School of Chemical Engineering, Oklahoma State University, Stillwater, OK, USA.School of Electrical and Computer Engineering, Helmerich Advanced Technology Research Center, Oklahoma State University, Stillwater, OK, USA.School of Chemical Engineering, Oklahoma State University, Stillwater, OK, USA ; School of Material Science and Engineering, Helmerich Advanced Technology Research Center, Oklahoma State University, Tulsa, OK, USA.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

24399874

Citation

Shahini, Aref, et al. "3D Conductive Nanocomposite Scaffold for Bone Tissue Engineering." International Journal of Nanomedicine, vol. 9, 2014, pp. 167-81.
Shahini A, Yazdimamaghani M, Walker KJ, et al. 3D conductive nanocomposite scaffold for bone tissue engineering. Int J Nanomedicine. 2014;9:167-81.
Shahini, A., Yazdimamaghani, M., Walker, K. J., Eastman, M. A., Hatami-Marbini, H., Smith, B. J., Ricci, J. L., Madihally, S. V., Vashaee, D., & Tayebi, L. (2014). 3D conductive nanocomposite scaffold for bone tissue engineering. International Journal of Nanomedicine, 9, 167-81. https://doi.org/10.2147/IJN.S54668
Shahini A, et al. 3D Conductive Nanocomposite Scaffold for Bone Tissue Engineering. Int J Nanomedicine. 2014;9:167-81. PubMed PMID: 24399874.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - 3D conductive nanocomposite scaffold for bone tissue engineering. AU - Shahini,Aref, AU - Yazdimamaghani,Mostafa, AU - Walker,Kenneth J, AU - Eastman,Margaret A, AU - Hatami-Marbini,Hamed, AU - Smith,Brenda J, AU - Ricci,John L, AU - Madihally,Sundar V, AU - Vashaee,Daryoosh, AU - Tayebi,Lobat, Y1 - 2013/12/24/ PY - 2014/1/9/entrez PY - 2014/1/9/pubmed PY - 2014/8/19/medline KW - PEDOT:PSS KW - bioactive glass nanoparticles KW - bone scaffold KW - conductive polymers KW - conductive scaffold KW - gelatin SP - 167 EP - 81 JF - International journal of nanomedicine JO - Int J Nanomedicine VL - 9 N2 - Bone healing can be significantly expedited by applying electrical stimuli in the injured region. Therefore, a three-dimensional (3D) ceramic conductive tissue engineering scaffold for large bone defects that can locally deliver the electrical stimuli is highly desired. In the present study, 3D conductive scaffolds were prepared by employing a biocompatible conductive polymer, ie, poly(3,4-ethylenedioxythiophene) poly(4-styrene sulfonate) (PEDOT:PSS), in the optimized nanocomposite of gelatin and bioactive glass. For in vitro analysis, adult human mesenchymal stem cells were seeded in the scaffolds. Material characterizations using hydrogen-1 nuclear magnetic resonance, in vitro degradation, as well as thermal and mechanical analysis showed that incorporation of PEDOT:PSS increased the physiochemical stability of the composite, resulting in improved mechanical properties and biodegradation resistance. The outcomes indicate that PEDOT:PSS and polypeptide chains have close interaction, most likely by forming salt bridges between arginine side chains and sulfonate groups. The morphology of the scaffolds and cultured human mesenchymal stem cells were observed and analyzed via scanning electron microscope, micro-computed tomography, and confocal fluorescent microscope. Increasing the concentration of the conductive polymer in the scaffold enhanced the cell viability, indicating the improved microstructure of the scaffolds or boosted electrical signaling among cells. These results show that these conductive scaffolds are not only structurally more favorable for bone tissue engineering, but also can be a step forward in combining the tissue engineering techniques with the method of enhancing the bone healing by electrical stimuli. SN - 1178-2013 UR - https://www.unboundmedicine.com/medline/citation/24399874/3D_conductive_nanocomposite_scaffold_for_bone_tissue_engineering_ L2 - https://dx.doi.org/10.2147/IJN.S54668 DB - PRIME DP - Unbound Medicine ER -