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Bacteria associated with yellow lupine grown on a metal-contaminated soil: in vitro screening and in vivo evaluation for their potential to enhance Cd phytoextraction.
Plant Biol (Stuttg). 2014 Sep; 16(5):988-96.PB

Abstract

In order to stimulate selection for plant-associated bacteria with the potential to improve Cd phytoextraction, yellow lupine plants were grown on a metal-contaminated field soil. It was hypothesised that growing these plants on this contaminated soil, which is a source of bacteria possessing different traits to cope with Cd, could enhance colonisation of lupine with potential plant-associated bacteria that could then be inoculated in Cd-exposed plants to reduce Cd phytotoxicity and enhance Cd uptake. All cultivable bacteria from rhizosphere, root and stem were isolated and genotypically and phenotypically characterised. Many of the rhizobacteria and root endophytes produce siderophores, organic acids, indole-3-acetic acid (IAA) and aminocyclopropane-1-carboxylate (ACC) deaminase, as well as being resistant to Cd and Zn. Most of the stem endophytes could produce organic acids (73.8%) and IAA (74.3%), however, only a minor fraction (up to 0.7%) were Cd or Zn resistant or could produce siderophores or ACC deaminase. A siderophore- and ACC deaminase-producing, highly Cd-resistant Rhizobium sp. from the rhizosphere, a siderophore-, organic acid-, IAA- and ACC deaminase-producing highly Cd-resistant Pseudomonas sp. colonising the roots, a highly Cd- and Zn-resistant organic acid and IAA-producing Clavibacter sp. present in the stem, and a consortium composed of these three strains were inoculated into non-exposed and Cd-exposed yellow lupine plants. Although all selected strains possessed promising in vitro characteristics to improve Cd phytoextraction, inoculation of none of the strains (i) reduced Cd phytotoxicity nor (ii) strongly affected plant Cd uptake. This work highlights that in vitro characterisation of bacteria is not sufficient to predict the in vivo behaviour of bacteria in interaction with their host plants.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Weyens N
Centre for Environmental Sciences, Hasselt University, Diepenbeek, Belgium.
Gielen M
No affiliation info available
Beckers B
No affiliation info available
Boulet J
No affiliation info available
van der Lelie D
No affiliation info available
Taghavi S
No affiliation info available
Carleer R
No affiliation info available
Vangronsveld J
No affiliation info available

MeSH

ActinomycetalesBiodegradation, EnvironmentalCadmiumLupinusPseudomonasRhizobiumRhizosphereSoilSoil PollutantsSymbiosis

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24400887

Citation

Weyens, N, et al. "Bacteria Associated With Yellow Lupine Grown On a Metal-contaminated Soil: in Vitro Screening and in Vivo Evaluation for Their Potential to Enhance Cd Phytoextraction." Plant Biology (Stuttgart, Germany), vol. 16, no. 5, 2014, pp. 988-96.
Weyens N, Gielen M, Beckers B, et al. Bacteria associated with yellow lupine grown on a metal-contaminated soil: in vitro screening and in vivo evaluation for their potential to enhance Cd phytoextraction. Plant Biol (Stuttg). 2014;16(5):988-96.
Weyens, N., Gielen, M., Beckers, B., Boulet, J., van der Lelie, D., Taghavi, S., Carleer, R., & Vangronsveld, J. (2014). Bacteria associated with yellow lupine grown on a metal-contaminated soil: in vitro screening and in vivo evaluation for their potential to enhance Cd phytoextraction. Plant Biology (Stuttgart, Germany), 16(5), 988-96. https://doi.org/10.1111/plb.12141
Weyens N, et al. Bacteria Associated With Yellow Lupine Grown On a Metal-contaminated Soil: in Vitro Screening and in Vivo Evaluation for Their Potential to Enhance Cd Phytoextraction. Plant Biol (Stuttg). 2014;16(5):988-96. PubMed PMID: 24400887.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bacteria associated with yellow lupine grown on a metal-contaminated soil: in vitro screening and in vivo evaluation for their potential to enhance Cd phytoextraction. AU - Weyens,N, AU - Gielen,M, AU - Beckers,B, AU - Boulet,J, AU - van der Lelie,D, AU - Taghavi,S, AU - Carleer,R, AU - Vangronsveld,J, Y1 - 2014/01/08/ PY - 2013/04/22/received PY - 2013/11/07/accepted PY - 2014/1/10/entrez PY - 2014/1/10/pubmed PY - 2015/4/7/medline KW - Cadmium KW - natural selection KW - phytoremediation KW - plant-associated bacteria KW - plant-bacteria interactions SP - 988 EP - 96 JF - Plant biology (Stuttgart, Germany) JO - Plant Biol (Stuttg) VL - 16 IS - 5 N2 - In order to stimulate selection for plant-associated bacteria with the potential to improve Cd phytoextraction, yellow lupine plants were grown on a metal-contaminated field soil. It was hypothesised that growing these plants on this contaminated soil, which is a source of bacteria possessing different traits to cope with Cd, could enhance colonisation of lupine with potential plant-associated bacteria that could then be inoculated in Cd-exposed plants to reduce Cd phytotoxicity and enhance Cd uptake. All cultivable bacteria from rhizosphere, root and stem were isolated and genotypically and phenotypically characterised. Many of the rhizobacteria and root endophytes produce siderophores, organic acids, indole-3-acetic acid (IAA) and aminocyclopropane-1-carboxylate (ACC) deaminase, as well as being resistant to Cd and Zn. Most of the stem endophytes could produce organic acids (73.8%) and IAA (74.3%), however, only a minor fraction (up to 0.7%) were Cd or Zn resistant or could produce siderophores or ACC deaminase. A siderophore- and ACC deaminase-producing, highly Cd-resistant Rhizobium sp. from the rhizosphere, a siderophore-, organic acid-, IAA- and ACC deaminase-producing highly Cd-resistant Pseudomonas sp. colonising the roots, a highly Cd- and Zn-resistant organic acid and IAA-producing Clavibacter sp. present in the stem, and a consortium composed of these three strains were inoculated into non-exposed and Cd-exposed yellow lupine plants. Although all selected strains possessed promising in vitro characteristics to improve Cd phytoextraction, inoculation of none of the strains (i) reduced Cd phytotoxicity nor (ii) strongly affected plant Cd uptake. This work highlights that in vitro characterisation of bacteria is not sufficient to predict the in vivo behaviour of bacteria in interaction with their host plants. SN - 1438-8677 UR - https://www.unboundmedicine.com/medline/citation/24400887/Bacteria_associated_with_yellow_lupine_grown_on_a_metal_contaminated_soil:_in_vitro_screening_and_in_vivo_evaluation_for_their_potential_to_enhance_Cd_phytoextraction_ DB - PRIME DP - Unbound Medicine ER -