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Novel core-shell cerium(IV)-immobilized magnetic polymeric microspheres for selective enrichment and rapid separation of phosphopeptides.
J Colloid Interface Sci 2014; 417:217-26JC

Abstract

In this work, novel magnetic polymeric core-shell structured microspheres with immobilized Ce(IV), Fe3O4@SiO2@PVPA-Ce(IV), were designed rationally and synthesized successfully via a facile route for the first time. Magnetic Fe3O4@SiO2 microspheres were first prepared by directly coating a thin layer of silica onto Fe3O4 magnetic particles using a sol-gel method, a poly(vinylphosphonic acid) (PVPA) shell was then coated on the Fe3O4@SiO2 microspheres to form Fe3O4@SiO2@PVPA microspheres through a radical polymerization reaction, and finally Ce(IV) ions were robustly immobilized onto the Fe3O4@SiO2@PVPA microspheres through strong chelation between Ce(IV) ions and phosphate moieties in the PVPA. The applicability of the Fe3O4@SiO2@PVPA-Ce(IV) microspheres for selective enrichment and rapid separation of phosphopeptides from proteolytic digests of standard and real protein samples was investigated. The results demonstrated that the core-shell structured Fe3O4@SiO2@PVPA-Ce(IV) microspheres with abundant Ce(IV) affinity sites and excellent magnetic responsiveness can effectively purify phosphopeptides from complex biosamples for MS detection taking advantage of the rapid magnetic separation and the selective affinity between Ce(IV) ions and phosphate moieties of the phosphopeptides. Furthermore, they can be effectively recycled and show good reusability, and have better performance than commercial TiO2 beads and homemade Fe3O4@PMAA-Ce(IV) microspheres. Thus the Fe3O4@SiO2@PVPA-Ce(IV) microspheres can benefit greatly the mass spectrometric qualitative analysis of phosphopeptides in phosphoproteome research.

Authors+Show Affiliations

State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Changchun 130022, China; University of Chinese Academy of Sciences, Beijing 100049, China.State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Changchun 130022, China; University of Chinese Academy of Sciences, Beijing 100049, China.State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Changchun 130022, China; University of Chinese Academy of Sciences, Beijing 100049, China.State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Changchun 130022, China. Electronic address: zjl@ciac.ac.cn.Changchun Institute Technology, Changchun 130012, China.State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Changchun 130022, China; College of Life Science, Shenzhen University, Shenzhen 518060, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24407680

Citation

Wang, Zhi-Gang, et al. "Novel Core-shell cerium(IV)-immobilized Magnetic Polymeric Microspheres for Selective Enrichment and Rapid Separation of Phosphopeptides." Journal of Colloid and Interface Science, vol. 417, 2014, pp. 217-26.
Wang ZG, Cheng G, Liu YL, et al. Novel core-shell cerium(IV)-immobilized magnetic polymeric microspheres for selective enrichment and rapid separation of phosphopeptides. J Colloid Interface Sci. 2014;417:217-26.
Wang, Z. G., Cheng, G., Liu, Y. L., Zhang, J. L., Sun, D. H., & Ni, J. Z. (2014). Novel core-shell cerium(IV)-immobilized magnetic polymeric microspheres for selective enrichment and rapid separation of phosphopeptides. Journal of Colloid and Interface Science, 417, pp. 217-26. doi:10.1016/j.jcis.2013.11.004.
Wang ZG, et al. Novel Core-shell cerium(IV)-immobilized Magnetic Polymeric Microspheres for Selective Enrichment and Rapid Separation of Phosphopeptides. J Colloid Interface Sci. 2014 Mar 1;417:217-26. PubMed PMID: 24407680.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Novel core-shell cerium(IV)-immobilized magnetic polymeric microspheres for selective enrichment and rapid separation of phosphopeptides. AU - Wang,Zhi-Gang, AU - Cheng,Gong, AU - Liu,Yan-Lin, AU - Zhang,Ji-Lin, AU - Sun,De-Hui, AU - Ni,Jia-Zuan, Y1 - 2013/11/19/ PY - 2013/09/24/received PY - 2013/11/05/revised PY - 2013/11/07/accepted PY - 2014/1/11/entrez PY - 2014/1/11/pubmed PY - 2014/7/23/medline KW - Ce(IV) KW - Magnetic polymeric microspheres KW - Phosphopeptides KW - Rapid separation KW - Selective enrichment SP - 217 EP - 26 JF - Journal of colloid and interface science JO - J Colloid Interface Sci VL - 417 N2 - In this work, novel magnetic polymeric core-shell structured microspheres with immobilized Ce(IV), Fe3O4@SiO2@PVPA-Ce(IV), were designed rationally and synthesized successfully via a facile route for the first time. Magnetic Fe3O4@SiO2 microspheres were first prepared by directly coating a thin layer of silica onto Fe3O4 magnetic particles using a sol-gel method, a poly(vinylphosphonic acid) (PVPA) shell was then coated on the Fe3O4@SiO2 microspheres to form Fe3O4@SiO2@PVPA microspheres through a radical polymerization reaction, and finally Ce(IV) ions were robustly immobilized onto the Fe3O4@SiO2@PVPA microspheres through strong chelation between Ce(IV) ions and phosphate moieties in the PVPA. The applicability of the Fe3O4@SiO2@PVPA-Ce(IV) microspheres for selective enrichment and rapid separation of phosphopeptides from proteolytic digests of standard and real protein samples was investigated. The results demonstrated that the core-shell structured Fe3O4@SiO2@PVPA-Ce(IV) microspheres with abundant Ce(IV) affinity sites and excellent magnetic responsiveness can effectively purify phosphopeptides from complex biosamples for MS detection taking advantage of the rapid magnetic separation and the selective affinity between Ce(IV) ions and phosphate moieties of the phosphopeptides. Furthermore, they can be effectively recycled and show good reusability, and have better performance than commercial TiO2 beads and homemade Fe3O4@PMAA-Ce(IV) microspheres. Thus the Fe3O4@SiO2@PVPA-Ce(IV) microspheres can benefit greatly the mass spectrometric qualitative analysis of phosphopeptides in phosphoproteome research. SN - 1095-7103 UR - https://www.unboundmedicine.com/medline/citation/24407680/Novel_core_shell_cerium_IV__immobilized_magnetic_polymeric_microspheres_for_selective_enrichment_and_rapid_separation_of_phosphopeptides_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9797(13)00988-0 DB - PRIME DP - Unbound Medicine ER -