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Inhibitory effects of oxatomide on intracellular Ca mobilization, Ca uptake and histamine release, using rat peritoneal mast cells.
Int Arch Allergy Appl Immunol. 1987; 83(4):348-53.IA

Abstract

Oxatomide at concentrations of 0.01-10 microM inhibited not only an increase in 45Ca uptake but also the intracellular Ca2+ release induced by compound 48/80 in rat peritoneal mast cells. At higher concentrations, ketotifen or other calcium antagonists caused similar inhibitory effects. However, the inhibitory effect of oxatomide on the 45Ca uptake into rat neonatal heart cells was much weaker than that of verapamil. Through image processing of quin 2-stained mast cells, it was revealed that oxatomide inhibited Ca2+ release from the intracellular store. Although oxatomide alone did not affect cAMP and cGMP contents in sensitized guinea pig lung samples, the drug effectively prevented changes in the nucleotide contents evoked by antigen challenge. These results suggest that the inhibitory effect of oxatomide on histamine release may be caused by a combination of prevention of Ca uptake, which is highly selective toward mast cells; inhibition of Ca2+ release from the intracellular Ca store, and elevation of the cAMP content in mast cells.

Authors

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Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

2440814

Citation

Tasaka, K, et al. "Inhibitory Effects of Oxatomide On Intracellular Ca Mobilization, Ca Uptake and Histamine Release, Using Rat Peritoneal Mast Cells." International Archives of Allergy and Applied Immunology, vol. 83, no. 4, 1987, pp. 348-53.
Tasaka K, Akagi M, Mio M, et al. Inhibitory effects of oxatomide on intracellular Ca mobilization, Ca uptake and histamine release, using rat peritoneal mast cells. Int Arch Allergy Appl Immunol. 1987;83(4):348-53.
Tasaka, K., Akagi, M., Mio, M., Miyoshi, K., & Nakaya, N. (1987). Inhibitory effects of oxatomide on intracellular Ca mobilization, Ca uptake and histamine release, using rat peritoneal mast cells. International Archives of Allergy and Applied Immunology, 83(4), 348-53.
Tasaka K, et al. Inhibitory Effects of Oxatomide On Intracellular Ca Mobilization, Ca Uptake and Histamine Release, Using Rat Peritoneal Mast Cells. Int Arch Allergy Appl Immunol. 1987;83(4):348-53. PubMed PMID: 2440814.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibitory effects of oxatomide on intracellular Ca mobilization, Ca uptake and histamine release, using rat peritoneal mast cells. AU - Tasaka,K, AU - Akagi,M, AU - Mio,M, AU - Miyoshi,K, AU - Nakaya,N, PY - 1987/1/1/pubmed PY - 1987/1/1/medline PY - 1987/1/1/entrez SP - 348 EP - 53 JF - International archives of allergy and applied immunology JO - Int Arch Allergy Appl Immunol VL - 83 IS - 4 N2 - Oxatomide at concentrations of 0.01-10 microM inhibited not only an increase in 45Ca uptake but also the intracellular Ca2+ release induced by compound 48/80 in rat peritoneal mast cells. At higher concentrations, ketotifen or other calcium antagonists caused similar inhibitory effects. However, the inhibitory effect of oxatomide on the 45Ca uptake into rat neonatal heart cells was much weaker than that of verapamil. Through image processing of quin 2-stained mast cells, it was revealed that oxatomide inhibited Ca2+ release from the intracellular store. Although oxatomide alone did not affect cAMP and cGMP contents in sensitized guinea pig lung samples, the drug effectively prevented changes in the nucleotide contents evoked by antigen challenge. These results suggest that the inhibitory effect of oxatomide on histamine release may be caused by a combination of prevention of Ca uptake, which is highly selective toward mast cells; inhibition of Ca2+ release from the intracellular Ca store, and elevation of the cAMP content in mast cells. SN - 0020-5915 UR - https://www.unboundmedicine.com/medline/citation/2440814/Inhibitory_effects_of_oxatomide_on_intracellular_Ca_mobilization_Ca_uptake_and_histamine_release_using_rat_peritoneal_mast_cells_ DB - PRIME DP - Unbound Medicine ER -