Tags

Type your tag names separated by a space and hit enter

Development of a wheat single gene FISH map for analyzing homoeologous relationship and chromosomal rearrangements within the Triticeae.
Theor Appl Genet. 2014 Mar; 127(3):715-30.TA

Abstract

A cytogenetic map of wheat was constructed using FISH with cDNA probes. FISH markers detected homoeology and chromosomal rearrangements of wild relatives, an important source of genes for wheat improvement. To transfer agronomically important genes from wild relatives to bread wheat (Triticum aestivum L., 2n = 6 x = 42, AABBDD) by induced homoeologous recombination, it is important to know the chromosomal relationships of the species involved. Fluorescence in situ hybridization (FISH) can be used to study chromosome structure. The genomes of allohexaploid bread wheat and other species from the Triticeae tribe are colinear to some extent, i.e., composed of homoeoloci at similar positions along the chromosomes, and with genic regions being highly conserved. To develop cytogenetic markers specific for genic regions of wheat homoeologs, we selected more than 60 full-length wheat cDNAs using BLAST against mapped expressed sequence tags and used them as FISH probes. Most probes produced signals on all three homoeologous chromosomes at the expected positions. We developed a wheat physical map with several cDNA markers located on each of the 14 homoeologous chromosome arms. The FISH markers confirmed chromosome rearrangements within wheat genomes and were successfully used to study chromosome structure and homoeology in wild Triticeae species. FISH analysis detected 1 U-6 U chromosome translocation in the genome of Aegilops umbellulata, showed colinearity between chromosome A of Ae. caudata and group-1 wheat chromosomes, and between chromosome arm 7S#3 L of Thinopyrum intermedium and the long arm of the group-7 wheat chromosomes.

Authors+Show Affiliations

Department of Plant Pathology, Wheat Genetics Resource Center, Kansas State University, Manhattan, KS, 66506, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24408375

Citation

Danilova, Tatiana V., et al. "Development of a Wheat Single Gene FISH Map for Analyzing Homoeologous Relationship and Chromosomal Rearrangements Within the Triticeae." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 127, no. 3, 2014, pp. 715-30.
Danilova TV, Friebe B, Gill BS. Development of a wheat single gene FISH map for analyzing homoeologous relationship and chromosomal rearrangements within the Triticeae. Theor Appl Genet. 2014;127(3):715-30.
Danilova, T. V., Friebe, B., & Gill, B. S. (2014). Development of a wheat single gene FISH map for analyzing homoeologous relationship and chromosomal rearrangements within the Triticeae. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 127(3), 715-30. https://doi.org/10.1007/s00122-013-2253-z
Danilova TV, Friebe B, Gill BS. Development of a Wheat Single Gene FISH Map for Analyzing Homoeologous Relationship and Chromosomal Rearrangements Within the Triticeae. Theor Appl Genet. 2014;127(3):715-30. PubMed PMID: 24408375.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of a wheat single gene FISH map for analyzing homoeologous relationship and chromosomal rearrangements within the Triticeae. AU - Danilova,Tatiana V, AU - Friebe,Bernd, AU - Gill,Bikram S, Y1 - 2014/01/10/ PY - 2013/08/16/received PY - 2013/12/13/accepted PY - 2014/1/11/entrez PY - 2014/1/11/pubmed PY - 2014/10/17/medline SP - 715 EP - 30 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 127 IS - 3 N2 - A cytogenetic map of wheat was constructed using FISH with cDNA probes. FISH markers detected homoeology and chromosomal rearrangements of wild relatives, an important source of genes for wheat improvement. To transfer agronomically important genes from wild relatives to bread wheat (Triticum aestivum L., 2n = 6 x = 42, AABBDD) by induced homoeologous recombination, it is important to know the chromosomal relationships of the species involved. Fluorescence in situ hybridization (FISH) can be used to study chromosome structure. The genomes of allohexaploid bread wheat and other species from the Triticeae tribe are colinear to some extent, i.e., composed of homoeoloci at similar positions along the chromosomes, and with genic regions being highly conserved. To develop cytogenetic markers specific for genic regions of wheat homoeologs, we selected more than 60 full-length wheat cDNAs using BLAST against mapped expressed sequence tags and used them as FISH probes. Most probes produced signals on all three homoeologous chromosomes at the expected positions. We developed a wheat physical map with several cDNA markers located on each of the 14 homoeologous chromosome arms. The FISH markers confirmed chromosome rearrangements within wheat genomes and were successfully used to study chromosome structure and homoeology in wild Triticeae species. FISH analysis detected 1 U-6 U chromosome translocation in the genome of Aegilops umbellulata, showed colinearity between chromosome A of Ae. caudata and group-1 wheat chromosomes, and between chromosome arm 7S#3 L of Thinopyrum intermedium and the long arm of the group-7 wheat chromosomes. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/24408375/Development_of_a_wheat_single_gene_FISH_map_for_analyzing_homoeologous_relationship_and_chromosomal_rearrangements_within_the_Triticeae_ L2 - https://dx.doi.org/10.1007/s00122-013-2253-z DB - PRIME DP - Unbound Medicine ER -